Nanog In situ reporter (knock in) Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:284140 Frum T, et al., TEAD4, YAP1 and WWTR1 prevent the premature onset of pluripotency prior to the 16-cell stage. Development. 2019 Sep 6;146(17):dev179861
Assay type:	In situ reporter (knock in)
MGI Accession ID:	MGI:6403729
Gene symbol:	Nanog
Gene name:	Nanog homeobox

Reporter:	GFP
	Direct Detection

Results

1E Merge upper ©

1E Nanog-GFP upper ©

Specimen 1E +/GFP: embryonic day 3.75; Nanog^tm1Hoch/Nanog⁺ (more

)
Note: Double labeled: green - Nanog; red - Sox2 (assay MGI:6403731). This embryo was the result of a cross between double heterozygotes; it might also be heterozygous for Pou5f1 (Oct4).

Structure	Level	Pattern	Image	Note
TS5: inner cell mass	Present	Ubiquitous	1E Merge upper , 1E Nanog-GFP upper

1E Merge lower ©

1E Nanog-GFP lower ©

Specimen 1E Nanog null: embryonic day 3.75; Nanog^tm1Hoch/Nanog^tm1Hoch (more

)
Note: Double labeled: green - Nanog; red - Sox2 (assay MGI:6403731). This embryo was the result of a cross between double heterozygotes; it might also be heterozygous for Pou5f1 (Oct4).

Structure	Level	Pattern	Image	Note
TS5: inner cell mass	Present	Ubiquitous	1E Nanog-GFP lower , 1E Merge lower

1G Merge upper ©

1G Nanog-GFP upper ©

Specimen 1G +/GFP: embryonic day 4.25; Nanog^tm1Hoch/Nanog⁺ (more

)
Note: Double labeled: green - Nanog; red - Sox2 (assay MGI:6403731). This embryo was the result of a cross between double heterozygotes; it might also be heterozygous for Pou5f1 (Oct4).

Structure	Level	Pattern	Image	Note
TS6: blastocyst	Present		1G Nanog-GFP upper , 1G Merge upper

Specimen 1G Nanog null: embryonic day 4.25; Nanog^tm1Hoch/Nanog^tm1Hoch (more

)
Note: Double labeled: green - Nanog; red - Sox2 (assay MGI:6403731). This embryo was the result of a cross between double heterozygotes; it might also be heterozygous for Pou5f1 (Oct4).

Structure	Level	Pattern	Image	Note
TS6: blastocyst	Present		1G Merge lower , 1G Nanog-GFP lower

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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