Wt1 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:284147 Velecela V, et al., Epicardial cell shape and maturation are regulated by Wt1 via transcriptional control of Bmp4. Development. 2019 Oct 17;146(20):dev178723
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:6401402
Gene symbol:	Wt1
Gene name:	WT1 transcription factor

Antibody:	anti-WT1 [CAN-R9(IHC)-56-2; ab89901]
Detection system:	Secondary antibody coupled to Alexa Fluor 488

Results

S3B Control ©

Specimen S3B - Control: embryonic day 15.5; Wt1^tm1.1Ndha/? (more

)
Note: Littermate control; exact genotype not reported. The section is also stained for sarcomeric/cardiac myosin (red) and Hoechst (blue).

Structure	Level	Pattern	Image	Note
TS23: visceral pericardium	Present		S3B Control	Expression is seen in the epicardium.

S3B epiWt1KO ©

Specimen S3B - epiWt1KO: embryonic day 15.5; Wt1^tm1.1Ndha/Wt1^tm1Nhsn, Tg(GATA5-cre)1Krc/0 (more

)
Note: Conditional mutant. The section is also stained for sarcomeric/cardiac myosin (red) and Hoechst (blue).

Structure	Level	Pattern	Image	Note
TS23: visceral pericardium	Absent		S3B epiWt1KO	No expression is seen in the epicardium.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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