About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:284147 Velecela V, et al., Epicardial cell shape and maturation are regulated by Wt1 via transcriptional control of Bmp4. Development. 2019 Oct 17;146(20):dev178723
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6401390
Gene symbol: Pdpn
Gene name: podoplanin
Antibody: Anti-Podoplanin (clone 8.1.1)
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 4C Control: embryonic day 15.5; Wt1tm1.1Ndha/? (more )
Note: Littermate control; exact genotype not reported. The section is also stained for sarcomeric myosin (red) and Hoechst (blue).
Structure Level Pattern Image Note
TS23: visceral pericardium Present 4C Pdpn Control Epicardial cells have a flattened and elongated morphology in the controls.
Specimen 4C epiWt1KO: embryonic day 15.5; Wt1tm1.1Ndha/Wt1tm1Nhsn, Tg(GATA5-cre)1Krc/0 (more )
Note: Conditional mutant. The section is also stained for sarcomeric myosin (red) and Hoechst (blue).
Structure Level Pattern Image Note
TS23: visceral pericardium Present 4C Pdpn epiWT1KO Epicardial cells have a cuboidal shape in the mutants.
Specimen 4F E11.5: embryonic day 11.5 (more )
Note: The section is also stained for sarcomeric myosin (red) and Hoechst (blue).
Structure Level Pattern Image Note
TS19: visceral pericardium Present 4F Pdpn E11.5 Epicardial cells have a cuboidal shape at this stage of development.
Specimen 4F E13.5: embryonic day 13.5 (more )
Note: The section is also stained for sarcomeric myosin (red) and Hoechst (blue).
Structure Level Pattern Image Note
TS21: visceral pericardium Present 4F Pdpn E13.5 Epicardial cells have begun to elongate at this stage of development.
Specimen 4F E15.5: embryonic day 15.5 (more )
Note: The section is also stained for sarcomeric myosin (red) and Hoechst (blue).
Structure Level Pattern Image Note
TS23: visceral pericardium Present 4F Pdpn E15.5 Epicardial cells have a flattened shape at this stage of development.
Specimen 4F E18.5: embryonic day 18.5 (more )
Note: The section is also stained for sarcomeric myosin (red) and Hoechst (blue).
Structure Level Pattern Image Note
TS26: visceral pericardium Present 4F Pdpn E18.5 A squamous epicardium is present at this stage of development.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
03/25/2025
MGI 6.24 		The Jackson Laboratory