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Gene Expression Data
Assay Details
Assay
Reference: J:283695 Quesada-Lopez T, et al., GPR120 controls neonatal brown adipose tissue thermogenic induction. Am J Physiol Endocrinol Metab. 2019 Nov 1;317(5):E742-E750
Assay type: RT-PCR
MGI Accession ID: MGI:6400775
Gene symbol: Ffar4
Gene name: free fatty acid receptor 4
Probe: Mm00725193_m1
Assay notes: TaqMan gene expression assay. The mRNA level was normalized to that of the reference control, 18S rRNA.
Results Image: 1A
 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified (b) Amount Genetic BackgroundMutant Allele(s)Sex Note
E19 E19.0 (a) TS26: brown fat Weak 0.5 µg; total RNA Swiss Not Specified interscapular fat
P0 P0 TS27: brown fat Weak 0.5 µg; total RNA Swiss Not Specified interscapular fat
P0.25 P0.25 TS27: brown fat Strong 0.5 µg; total RNA Swiss Not Specified interscapular fat
P0.5 P0.5 TS27: brown fat Strong 0.5 µg; total RNA Swiss Not Specified interscapular fat
P1 P1 TS27: brown fat Strong 0.5 µg; total RNA Swiss Not Specified interscapular fat
P2 P2 TS27: brown fat Strong 0.5 µg; total RNA Swiss Not Specified interscapular fat
P7 P7 TS28: brown fat Strong 0.5 µg; total RNA Swiss Not Specified interscapular fat
P14 P14 TS28: brown fat Strong 0.5 µg; total RNA Swiss Not Specified interscapular fat
P21 P21 TS28: brown fat Present 0.5 µg; total RNA Swiss Not Specified interscapular fat
A P70 TS28: brown fat Present 0.5 µg; total RNA Swiss Not Specified interscapular fat
E19 E19.0 (a) TS26: liver Trace 0.5 µg; total RNA Swiss Not Specified
P0.5 P0.5 TS27: liver Trace 0.5 µg; total RNA Swiss Not Specified
P1 P1 TS27: liver Trace 0.5 µg; total RNA Swiss Not Specified
P2 P2 TS27: liver Trace 0.5 µg; total RNA Swiss Not Specified
P7 P7 TS28: liver Trace 0.5 µg; total RNA Swiss Not Specified
P14 P14 TS28: liver Trace 0.5 µg; total RNA Swiss Not Specified
P21 P21 TS28: liver Trace 0.5 µg; total RNA Swiss Not Specified
A P70 TS28: liver Trace 0.5 µg; total RNA Swiss Not Specified
E19 E19.0 (a) TS26: duodenum Trace 0.5 µg; total RNA Swiss Not Specified
P0.5 P0.5 TS27: duodenum Present 0.5 µg; total RNA Swiss Not Specified
P1 P1 TS27: duodenum Present 0.5 µg; total RNA Swiss Not Specified
P2 P2 TS27: duodenum Present 0.5 µg; total RNA Swiss Not Specified
P7 P7 TS28: duodenum Present 0.5 µg; total RNA Swiss Not Specified
P14 P14 TS28: duodenum Present 0.5 µg; total RNA Swiss Not Specified
P21 P21 TS28: duodenum Present 0.5 µg; total RNA Swiss Not Specified
A P70 TS28: duodenum Present 0.5 µg; total RNA Swiss Not Specified
E19 E19.0 (a) TS26: jejunum Trace 0.5 µg; total RNA Swiss Not Specified
P0.5 P0.5 TS27: jejunum Present 0.5 µg; total RNA Swiss Not Specified
P1 P1 TS27: jejunum Present 0.5 µg; total RNA Swiss Not Specified
P2 P2 TS27: jejunum Present 0.5 µg; total RNA Swiss Not Specified
P7 P7 TS28: jejunum Present 0.5 µg; total RNA Swiss Not Specified
P14 P14 TS28: jejunum Present 0.5 µg; total RNA Swiss Not Specified
P21 P21 TS28: jejunum Present 0.5 µg; total RNA Swiss Not Specified
A P70 TS28: jejunum Present 0.5 µg; total RNA Swiss Not Specified
E19 E19.0 (a) TS26: ileum Trace 0.5 µg; total RNA Swiss Not Specified
P0.5 P0.5 TS27: ileum Present 0.5 µg; total RNA Swiss Not Specified
P1 P1 TS27: ileum Present 0.5 µg; total RNA Swiss Not Specified
P2 P2 TS27: ileum Present 0.5 µg; total RNA Swiss Not Specified
P7 P7 TS28: ileum Present 0.5 µg; total RNA Swiss Not Specified
P14 P14 TS28: ileum Present 0.5 µg; total RNA Swiss Not Specified
P21 P21 TS28: ileum Present 0.5 µg; total RNA Swiss Not Specified
A P70 TS28: ileum Present 0.5 µg; total RNA Swiss Not Specified
E19 E19.0 (a) TS26: colon Trace 0.5 µg; total RNA Swiss Not Specified
P0.5 P0.5 TS27: colon Present 0.5 µg; total RNA Swiss Not Specified
P1 P1 TS27: colon Present 0.5 µg; total RNA Swiss Not Specified
P2 P2 TS27: colon Present 0.5 µg; total RNA Swiss Not Specified
P7 P7 TS28: colon Present 0.5 µg; total RNA Swiss Not Specified
P14 P14 TS28: colon Present 0.5 µg; total RNA Swiss Not Specified
P21 P21 TS28: colon Present 0.5 µg; total RNA Swiss Not Specified
A P70 TS28: colon Present 0.5 µg; total RNA Swiss Not Specified
Notes:
(a) Age of embryo at noon of plug day not specified in reference.
(b) Levels of expression in brown fat far exceed those seen elsewere; it is turned on strongly in the first days of life, progressively declining to adult levels (which is still higher than levels seen elsewhere). Expression in liver is only minimal. In intestine expression levels in the adult are higher than those seen in the neonate.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
MGI 6.24 		The Jackson Laboratory