Probe:	Daam1 cDNA3
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS17: olfactory epithelium	Present		3A
TS17: brain mantle layer	Present	Regionally restricted	3A	Expression is in a thin layer in the cerebral cortex.
TS17: forebrain	Present	Regionally restricted	3A	Expression is in the ganglionic eminence.

Structure	Level	Pattern	Image	Note
TS22: cortical plate	Present	Regionally restricted	3B , 3E	Expression is in the outer layer.
TS22: cerebral cortex intermediate zone	Present	Regionally restricted	3B , 3E	Expression is in the inner layer between the cortical plate and the ventricular zone.

Structure	Level	Pattern	Image	Note
TS23: cerebral cortex subventricular zone	Present		3F , 3C
TS23: cortical plate	Present		3F , 3C
TS23: cerebral cortex intermediate zone	Present	Regionally restricted	3C , 3F	Expression is absent in the outer intermediate zone.
TS23: cerebral cortex ventricular layer	Present	Regionally restricted	3C , 3F	Expression is absent at the inner ventricular zone next to the ependyma.

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex subventricular zone	Present		3H , 3D
TS24: cortical plate	Present		3H , 3D
TS24: cerebral cortex intermediate zone	Present	Regionally restricted	3H , 3D	Expression is absent in the outer intermediate zone.
TS24: cerebral cortex ventricular layer	Present	Regionally restricted	3D , 3H	Expression is absent at the inner ventricular zone next to the ependyma.

Structure	Level	Pattern	Image	Note
TS22: olfactory epithelium	Present	Regionally restricted	3I	Expression is in the middle layer and is absent in the apical layer.

Structure	Level	Pattern	Image	Note
TS22: neural retina	Present		3J

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present		3K

Structure	Level	Pattern	Image	Note
TS17: neural tube lateral wall	Present	Regionally restricted	3L	Expression is in the mantle layer.

Structure	Level	Pattern	Image	Note
TS19: neural tube mantle layer	Present	Graded	3M	Expression is stronger in the dorsal half.
TS19: dorsal root ganglion	Weak		3M
TS19: neural tube ventricular layer	Absent		3M

Structure	Level	Pattern	Image	Note
TS22: spinal cord	Present	Uniform	3N	Uniform expression but it is absent from the outer layer of the spinal cord.
TS22: spinal cord mantle layer	Present	Regionally restricted	3N	Expression is absent in the outer layer of the mantle zone.
TS22: dorsal root ganglion	Present	Uniform	3N