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Gene Expression Data
Assay Details
Assay
Reference: J:187714 Zheng X, et al., Dynamic regulation of the cerebral cavernous malformation pathway controls vascular stability and growth. Dev Cell. 2012 Aug 14;23(2):342-55
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:6394089
Gene symbol: Ccm2l
Gene name: cerebral cavernous malformation 2-like
Reporter: GFP
Antibody: anti-GFP (Abcam)
Results
Specimen not shown: embryonic day 9.5; Ccm2ltm1Mlkn/Ccm2l+ (more )
Structure Level Pattern Image Note
TS15: endocardial lining Strong
Specimen 2G: embryonic day 10.5; Ccm2ltm1Mlkn/Ccm2l+ (more )
Note: Double labeled: green - Ccm2l; red - Pecam1 (assay MGI:6394094).
Structure Level Pattern Image Note
TS17: heart endothelium Present 2G
TS17: endocardial lining Strong Regionally restricted 2G Expression was detected in a small subset of endothelial cells.
TS17: cardiac muscle tissue Absent 2G
Specimen 2H: embryonic day 11.5; Ccm2ltm1Mlkn/Ccm2l+ (more )
Note: Double labeled: green - Ccm2l; red - Pecam1 (assay MGI:6394094).
Structure Level Pattern Image Note
TS19: heart endothelium Present 2H
TS19: endocardial lining Strong 2H Expression level in endocardium peaked at E11.5 (20% of nuclei were GFP+).
TS19: cardiac muscle tissue Absent 2H
Specimen 2I: embryonic day 12.5; Ccm2ltm1Mlkn/Ccm2l+ (more )
Note: Double labeled: green - Ccm2l; red - Pecam1 (assay MGI:6394094).
Structure Level Pattern Image Note
TS20: heart endothelium Present 2I
TS20: endocardial lining Present 2I Expression was detected in a small subset of endothelial cells.
TS20: cardiac muscle tissue Absent 2I
Specimen 2J: embryonic day 15.5; Ccm2ltm1Mlkn/Ccm2l+ (more )
Note: Double labeled: green - Ccm2l; red - Pecam1 (assay MGI:6394094).
Structure Level Pattern Image Note
TS23: heart endothelium Present 2J
TS23: endocardial lining Present 2J Expression was detected in a small subset of endothelial cells.
TS23: cardiac muscle tissue Absent 2J
Specimen 2L: embryonic day 10.5; Ccm2ltm1Mlkn/Ccm2l+ (more )
Note: Double labeled: green - Ccm2l; red - Pecam1 (assay MGI:6394094).
Structure Level Pattern Image Note
TS17: dorsal aorta Strong 2L Strong expression was detected in the endothelial cells.
Specimen 2M: embryonic day 10.5; Ccm2ltm1Mlkn/Ccm2l+ (more )
Note: Double labeled: green - Ccm2l; red - Pecam1 (assay MGI:6394094).
Structure Level Pattern Image Note
TS17: dorsal aorta Strong 2M Strong expression was detected in the endothelial cells.
Specimen 2N: embryonic day 10.5; Ccm2ltm1Mlkn/Ccm2l+ (more )
Note: Double labeled: green - Ccm2l; red - Pecam1 (assay MGI:6394094).
Structure Level Pattern Image Note
TS17: cardinal vein Strong 2N Strong expression was detected in the endothelial cells.
Specimen 2P: embryonic day 10.5; Ccm2ltm1Mlkn/Ccm2l+ (more )
Note: Double labeled: green - Ccm2l; red - Pecam1 (assay MGI:6394094).
Structure Level Pattern Image Note
TS17: future spinal cord vascular element Present Regionally restricted 2P Expression was detected in endothelial cells invading the neural tube.
Specimen 2Q: embryonic day 10.5; Ccm2ltm1Mlkn/Ccm2l+ (more )
Note: Double labeled: green - Ccm2l; red - Pecam1 (assay MGI:6394094).
Structure Level Pattern Image Note
TS17: future spinal cord vascular element Present Regionally restricted 2Q Expression was detected in endothelial cells invading the neural tube.
Specimen 2S: postnatal day 6; Ccm2ltm1Mlkn/Ccm2l+ (more )
Note: Double labeled: green - Ccm2l; red - Pecam1 (assay MGI:6394094).
Structure Level Pattern Image Note
TS28: retina blood vessel Present 2T , 2S Expression was detected in the endothelial cells of most retina vessels.
Specimen S1E left: embryonic day 10.5; Ccm2ltm1Mlkn/Ccm2l+ (more )
Note: Double labeled: green - Ccm2l; red - BrdU.
Structure Level Pattern Image Note
TS17: blood vessel Present S1E Overlay left , S1E GFP left GFP+ endothelial cells did not exhibit an increase in BrdU uptake.
Specimen S1E center: embryonic day 10.5; Ccm2ltm1Mlkn/Ccm2l+ (more )
Note: Double labeled: green - Ccm2l; red - BrdU.
Structure Level Pattern Image Note
TS17: blood vessel Present S1E Overlay center , S1E GFP center GFP+ endothelial cells did not exhibit an increase in BrdU uptake.
Specimen S1E right: embryonic day 10.5; Ccm2ltm1Mlkn/Ccm2l+ (more )
Note: Double labeled: green - Ccm2l; red - BrdU.
Structure Level Pattern Image Note
TS17: blood vessel Present S1E Overlay right , S1E GFP right GFP+ endothelial cells did not exhibit an increase in BrdU uptake.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/30/2024
MGI 6.23 		The Jackson Laboratory