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Gene Expression Data
Assay Details
Assay
Reference: J:266963 Saito T, et al., A tandem repeat array in IG-DMR is essential for imprinting of paternal allele at the Dlk1-Dio3 domain during embryonic development. Hum Mol Genet. 2018 Sep 15;27(18):3283-3292
Assay type: RT-PCR
MGI Accession ID: MGI:6393494
Gene symbol: Rtl1
Gene name: retrotransposon Gaglike 1
Probe: Rtl1-ex1c-F2, Rtl1-RT-R
Probe preparation: labelled with SYBR green
Assay notes: Quantitative RT-PCR. Relative expression is shown normalized to Actb expression. Rtl1 is an imprinted gene; it is expressed only from the paternally inherited allele.
Results Image: 4A Rtl1
 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified Amount Genetic BackgroundMutant Allele(s)Sex Note
WT E14.5 TS22: embryo Present Not Specified; total RNA involves: C57BL/6 * DBA/2 Not Specified
IG-DMRdeltaRep/+ E14.5 TS22: embryo Present (a) Not Specified; total RNA involves: C57BL/6 * DBA/2 Igdmrem1Takas/Igdmr+Not Specified Maternally inherited IG-DMRdeltaRep.
IG-DMR+/deltaRep E14.5 TS22: embryo Weak (b) Not Specified; total RNA involves: C57BL/6 * DBA/2 Igdmrem1Takas/Igdmr+Not Specified Paternally inherited IG-DMRdeltaRep.
- Other lane(s) in blot assay different gene(s)
Notes:
(a) Expression levels were similar to WT.
(b) Expression was 0.09-fold of WT levels.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/30/2024
MGI 6.23 		The Jackson Laboratory