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Gene Expression Data
Assay Details
Assay
Reference: J:267832 McInturff S, et al., Characterization of spatial and temporal development of Type I and Type II hair cells in the mouse utricle using new cell-type-specific markers. Biol Open. 2018 Nov 19;7(11):bio038083
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6393312
Gene symbol: Calb2
Gene name: calbindin 2
Antibody: anti-Calretinin (MAB1568; clone 6B8.2)
Detection system: Secondary antibody coupled to Alexa Fluor
Assay notes: Two antibodies were described: MGI:5447969 and MGI:6209703; the one used in this assay was not detailed.
Results
Specimen 4C: postnatal day 64 (more )
Note: Double labeled: magenta - Calb2; green - Anxa4 (assay MGI:6393292).
Structure Level Pattern Image Note
TS28: utricle Present 4C , 4C' There was broad overlap of Anxa4 expression with the Type II hair cell marker, Calb2.
Specimen 4D: postnatal day 64 (more )
Note: Double labeled: magenta - Calb2; green - Mapt (assay MGI:6393311).
Structure Level Pattern Image Note
TS28: utricle Present 4D' , 4D There was broad overlap of Mapt expression with the Type II hair cell marker, Calb2.
Specimen 5C: postnatal day 70 (more )
Note: Double labeled: magenta - Calb2; green - Spp1 (assay MGI:6393438).
Structure Level Pattern Image Note
TS28: utricle Present 5C There was no overlap of Spp1 expression with the Type II hair cell marker, Calb2.
Specimen 7 P0: postnatal day 0 (more )
Note: Double labeled: green - Calb2; magenta - Myo7a (assay MGI:6393446).
Structure Level Pattern Image Note
TS27: utricle Present 7_Calb2 P0 , 7_Calb2 Myo7a P0 At P0, Calb2 is broadly expressed in nearly all hair cells, which were labeled by the pan-hair cell marker Myo7a.
Specimen 7 P0 orthogonal: postnatal day 0 (more )
Note: Double labeled: green - Calb2; magenta - Myo7a (assay MGI:6393446).
Structure Level Pattern Image Note
TS27: utricle Present 7_Calb2 Orthogonal P0 At P0, Calb2 is broadly expressed in nearly all hair cells, which were labeled by the pan-hair cell marker Myo7a.
Specimen 7 P12: postnatal day 12 (more )
Note: Double labeled: green - Calb2; magenta - Myo7a (assay MGI:6393446).
Structure Level Pattern Image Note
TS28: utricle Present 7_Calb2 P12 , 7_Calb2 Myo7a P12 At P12, Calb2 was expressed in over 80% of Type II hair cells and 30% Type I hair cells.
Specimen 7 P12 orthogonal: postnatal day 12 (more )
Note: Double labeled: green - Calb2; magenta - Myo7a (assay MGI:6393446).
Structure Level Pattern Image Note
TS28: utricle Present 7_Calb2 Orthogonal P12 At P12, Calb2 was expressed in over 80% of Type II hair cells and 30% Type I hair cells.
Specimen 7 P64: postnatal day 64 (more )
Note: Double labeled: green - Calb2; magenta - Myo7a (assay MGI:6393446).
Structure Level Pattern Image Note
TS28: utricle Present 7_Calb2 P64 , 7_Calb2 Myo7a P64 At P64, Calb2 was expressed in 80% of Type II hair cells but only 3.5% of Type I hair cells.
Specimen 7 P64 orthogonal: postnatal day 64 (more )
Note: Double labeled: green - Calb2; magenta - Myo7a (assay MGI:6393446).
Structure Level Pattern Image Note
TS28: utricle Present 7_Calb2 Orthogonal P64 At P64, Calb2 was expressed in 80% of Type II hair cells but only 3.5% of Type I hair cells.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/07/2024
MGI 6.23 		The Jackson Laboratory