Antibody:	Anti-Annexin A4 (AF4146)
Detection system:	Secondary antibody coupled to Alexa Fluor

Structure	Level	Pattern	Image	Note
TS28: utricle	Present		4A , 4A'	Expression was detected in a subset of hair cells, co-localized with the pan-hair cell marker Myo7a. Expression was localized to cell and nuclear membranes.

Structure	Level	Pattern	Image	Note
TS28: utricle	Present		4A"	Expression was detected in a subset of hair cells, co-localized with the pan-hair cell marker Myo7a. Expression was localized to cell and nuclear membranes. Based on cell morphology, the cell type was consistent with Type II hair cells.

Structure	Level	Pattern	Image	Note
TS28: utricle	Present		4C' , 4C	There was broad overlap of Anxa4 expression with the Type II hair cell marker, Calb2.

Structure	Level	Pattern	Image	Note
TS27: utricle	Present		7_Anxa P0 , 7_Anxa Myo7a P0	At P0, Anxa4 is broadly expressed in nearly all hair cells, which were labeled by the pan-hair cell marker Myo7a.

Structure	Level	Pattern	Image	Note
TS27: utricle	Present		7_Anxa Orthogonal P0	At P0, Anxa4 is broadly expressed in nearly all hair cells, which were labeled by the pan-hair cell marker Myo7a.

Structure	Level	Pattern	Image	Note
TS28: utricle	Present		7_Anxa Myo7a P12 , 7_Anxa P12	At P12, Anxa4 was expressed in fewer hair cells, which were labeled by the pan-hair cell marker Myo7a. Based on hair cell morphology, greater than 65% of Type II hair cells were Anxa4-immunopositive, with a decreasing expression in Type I hair cells.

Structure	Level	Pattern	Image	Note
TS28: utricle	Present		7_Anxa Orthogonal P12	At P12, Anxa4 was expressed in fewer hair cells, which were labeled by the pan-hair cell marker Myo7a. Based on hair cell morphology, greater than 65% of Type II hair cells were Anxa4-immunopositive, with a decreasing expression in Type I hair cells.

Structure	Level	Pattern	Image	Note
TS28: utricle	Present		7_Anxa Myo7a P64 , 7_Anxa P64	At P64, Anxa4 was expressed in fewer hair cells, which were labeled by the pan-hair cell marker Myo7a. Based on hair cell morphology, greater than 65% of Type II hair cells were Anxa4-immunopositive, with a decreasing expression in Type I hair cells.

Structure	Level	Pattern	Image	Note
TS28: utricle	Present		7_Anxa Orthogonal P64	At P64, Anxa4 was expressed in fewer hair cells, which were labeled by the pan-hair cell marker Myo7a. Based on hair cell morphology, greater than 65% of Type II hair cells were Anxa4-immunopositive, with a decreasing expression in Type I hair cells.