Gene Expression Data
Assay Details
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Assay
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Reference:
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J:32935
Collignon J, et al., Relationship between asymmetric nodal expression and the direction of embryonic turning. Nature. 1996 May 9;381(6578):155-8
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:6392914
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Gene symbol:
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Nodal
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Gene name:
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nodal growth differentiation factor
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Reporter:
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lacZ
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Direct Detection
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Results
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Specimen
1b1:
embryonic day 7.5; Nodaltm1Rob/Nodal +
(more  )
Note: younger embryo
Specimen
1b1:
(close  )
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Genetic Background:
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involves: 129S/SvEv
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Mutant Allele(s):
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Nodaltm1Rob/Nodal+
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Age:
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embryonic day 7.5
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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younger embryo
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Specimen
1b2:
embryonic day 7.5; Nodaltm1Rob/Nodal +
(more )
Specimen
1b2:
(close )
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Genetic Background:
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involves: 129S/SvEv
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Mutant Allele(s):
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Nodaltm1Rob/Nodal+
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Age:
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embryonic day 7.5
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Sex:
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Not Specified
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Type:
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whole mount
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Specimen
1b3:
embryonic day 7.5; Nodaltm1Rob/Nodal +
(more )
Specimen
1b3:
(close )
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Genetic Background:
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involves: 129S/SvEv
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Mutant Allele(s):
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Nodaltm1Rob/Nodal+
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Age:
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embryonic day 7.5
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Sex:
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Not Specified
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Type:
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whole mount
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Specimen
1b4:
embryonic day 7.5; Nodaltm1Rob/Nodal +
(more )
Specimen
1b4:
(close )
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Genetic Background:
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involves: 129S/SvEv
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Mutant Allele(s):
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Nodaltm1Rob/Nodal+
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Age:
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embryonic day 7.5
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Sex:
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Not Specified
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Type:
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whole mount
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Specimen
1b5:
embryonic day 7.5; Nodaltm1Rob/Nodal +
(more )
Note: headfold stage
Specimen
1b5:
(close )
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Genetic Background:
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involves: 129S/SvEv
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Mutant Allele(s):
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Nodaltm1Rob/Nodal+
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Age:
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embryonic day 7.5
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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headfold stage
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| Structure |
Level |
Pattern |
Image |
Note |
| TS11: node |
Present |
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1b5
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Specimen
1d:
embryonic day 7.5; Nodaltm1Rob/Nodal +
(more )
Note: headfold stage
Specimen
1d:
(close )
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Genetic Background:
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involves: 129S/SvEv
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Mutant Allele(s):
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Nodaltm1Rob/Nodal+
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Age:
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embryonic day 7.5
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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headfold stage
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| Structure |
Level |
Pattern |
Image |
Note |
| TS11: node |
Present |
Regionally restricted |
1d
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Expression extends more posteriorly, forming a continuous boundary around the notochordal plate.
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Specimen
1f:
embryonic day 8.5; Nodaltm1Rob/Nodal +
(more )
Note: 8 somites
Specimen
1f:
(close )
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Genetic Background:
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involves: 129S/SvEv
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Mutant Allele(s):
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Nodaltm1Rob/Nodal+
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Age:
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embryonic day 8.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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8 somites
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: node |
Present |
Regionally restricted |
1f
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Pronounced asymmetrical localization. More intense staining on the left side (approximately twice the number of positive cells against the right).
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Specimen
2a:
embryonic day 8.0; Nodaltm1Rob/Nodal +
(more )
Note: 4 somites
Specimen
2a:
(close )
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Genetic Background:
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involves: 129S/SvEv
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Mutant Allele(s):
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Nodaltm1Rob/Nodal+
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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4 somites
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: node |
Present |
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2a
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| TS12: lateral plate mesoderm |
Present |
Regionally restricted |
2a
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Expressed on the left side of the embryo, with a rostral limit at the position of the forming heart.
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Specimen
2b:
embryonic day 8.0; Nodaltm1Rob/Nodal +
(more )
Note: 4 somites
Specimen
2b:
(close )
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Genetic Background:
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involves: 129S/SvEv
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Mutant Allele(s):
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Nodaltm1Rob/Nodal+
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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4 somites
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: node |
Present |
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2b
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| TS12: lateral plate mesoderm |
Present |
Regionally restricted |
2b
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Expressed on the left side of the embryo, with a rostral limit at the position of the forming heart.
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Specimen
2c:
embryonic day 8.0; Nodaltm1Rob/Nodal +
(more )
Note: 5-6 somites
Specimen
2c:
(close )
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Genetic Background:
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involves: 129S/SvEv
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Mutant Allele(s):
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Nodaltm1Rob/Nodal+
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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5-6 somites
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: node |
Present |
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2c
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| TS12: somatopleure |
Present |
Regionally restricted |
2c
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Expressed on the left side of the embryo.
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| TS12: splanchnopleure |
Present |
Regionally restricted |
2c
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Expressed on the left side of the embryo.
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Specimen
2d:
embryonic day 8.0; Nodaltm1Rob/Nodal +
(more )
Note: 5-6 somites
Specimen
2d:
(close )
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Genetic Background:
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involves: 129S/SvEv
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Mutant Allele(s):
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Nodaltm1Rob/Nodal+
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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5-6 somites
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: lateral plate mesoderm |
Present |
Regionally restricted |
2d
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Confined to the prospective left side of the embryo, extending rostrally from the primitive streak to a position coincident with the caudal region of the developing heart tube.
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Specimen
2e:
embryonic day 8.5; Nodaltm1Rob/Nodal +
(more )
Note: 7-8 somites
Specimen
2e:
(close )
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Genetic Background:
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involves: 129S/SvEv
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Mutant Allele(s):
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Nodaltm1Rob/Nodal+
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Age:
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embryonic day 8.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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7-8 somites
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: node |
Present |
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2e
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| TS13: lateral plate mesoderm |
Present |
Regionally restricted |
2e
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Expressed on the left side of the embryo, extending into the dorsal wall of the pericardiac cavity adjacent to the myocardial layer.
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| TS13: pericardium |
Present |
Regionally restricted |
2e
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Expressed on the left side of the embryo, extending into the dorsal wall of the pericardiac cavity adjacent to the myocardial layer.
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Specimen
3a left:
(close )
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Genetic Background:
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involves: 129S/SvEv * FVB/N
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Mutant Allele(s):
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Invsinv/Invsinv
Nodaltm1Rob/Nodal+
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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8-12 somites
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: lateral plate mesoderm |
Present |
Regionally restricted |
3a left
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Expressed in the prospective right-hand side of the embryo. Showed a rostral truncation of the expression domain below the level of the pericardia! cavity (5 out of 7 mutants).
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Specimen
3a right:
embryonic day 8.5; Nodaltm1Rob/Nodal +
(more )
Note: 8-12 somites
Specimen
3a right:
(close )
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Genetic Background:
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involves: 129S/SvEv * FVB/N
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Mutant Allele(s):
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Nodaltm1Rob/Nodal+
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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8-12 somites
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: lateral plate mesoderm |
Present |
Regionally restricted |
3a right
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Expressed on the left side of the embryo, extending into the dorsal wall of the pericardiac cavity adjacent to the myocardial layer.
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Specimen
3b:
(close )
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Genetic Background:
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involves: 129S/SvEv * FVB/N
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Mutant Allele(s):
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Invsinv/Invsinv
Nodaltm1Rob/Nodal+
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: lateral plate mesoderm |
Present |
Regionally restricted |
3b
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Expressed on the right side of the embryo, extending into the dorsal wall of the pericardiac cavity adjacent to the myocardial layer (2 out of 7 mutants).
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Specimen
3c:
(close )
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Genetic Background:
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involves: 129S/SvEv * FVB/N
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Mutant Allele(s):
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Invsinv/Invsinv
Nodaltm1Rob/Nodal+
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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whole mount
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: lateral plate mesoderm |
Present |
Regionally restricted |
3c
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Expressed in the prospective right-hand side of the embryo. Showed a rostral truncation of the expression domain below the level of the pericardia! cavity (5 out of 7 mutants).
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Specimen
3d:
(close )
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Genetic Background:
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involves: 129S/SvEv * FVB/N
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Mutant Allele(s):
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Invsinv/Invsinv
Nodaltm1Rob/Nodal+
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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whole mount
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: lateral plate mesoderm |
Present |
Regionally restricted |
3d
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Expressed in the prospective right-hand side of the embryo. Showed a rostral truncation of the expression domain below the level of the pericardia! cavity (5 out of 7 mutants).
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Specimen
4a left:
embryonic day 8.5; Nodaltm1Rob/Nodal +
(more )
Specimen
4a left:
(close )
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Genetic Background:
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involves: 129S/SvEv * 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Nodaltm1Rob/Nodal+
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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whole mount
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: lateral plate mesoderm |
Present |
Regionally restricted |
4a left
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Expressed on the left side of the embryo.
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Specimen
4a middle:
(close )
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Genetic Background:
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involves: 129S/SvEv * 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Foxa2tm1Jrt/Foxa2+
Nodaltm1Rob/Nodal+
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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whole mount
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: lateral plate mesoderm |
Present |
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4a middle
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Abnornal bilateral expression, although stronger staining on the prospective left side.
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Specimen
4a right:
(close )
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Genetic Background:
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involves: 129S/SvEv * 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Foxa2tm1Jrt/Foxa2+
Nodaltm1Rob/Nodal+
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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whole mount
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: lateral plate mesoderm |
Present |
|
4a right
|
Abnornal bilateral expression, although stronger staining on the prospective left side.
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Specimen
4b:
(close )
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Genetic Background:
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involves: 129S/SvEv * 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Foxa2tm1Jrt/Foxa2+
Nodaltm1Rob/Nodal+
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Age:
|
embryonic day 8.5
|
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Sex:
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Not Specified
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Type:
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section from whole mount
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|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
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Paraffin
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Specimen
4c:
embryonic day 8.5; Nodaltm1Rob/Nodal +
(more )
Specimen
4c:
(close )
|
Genetic Background:
|
involves: 129S/SvEv * 129S1/Sv * 129X1/SvJ
|
|
Mutant Allele(s):
|
Nodaltm1Rob/Nodal+
|
|
Age:
|
embryonic day 8.5
|
|
Sex:
|
Not Specified
|
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Type:
|
whole mount
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS13: lateral plate mesoderm |
Present |
Regionally restricted |
4c
|
Expressed on the left side of the embryo, extending into the dorsal wall of the pericardiac cavity adjacent to the myocardial layer.
|
Specimen
4d:
(close )
|
Genetic Background:
|
involves: 129S/SvEv * 129S1/Sv * 129X1/SvJ
|
|
Mutant Allele(s):
|
Foxa2tm1Jrt/Foxa2+
Nodaltm1Rob/Nodal+
|
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Age:
|
embryonic day 8.5
|
|
Sex:
|
Not Specified
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Type:
|
whole mount
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS13: lateral plate mesoderm |
Present |
Regionally restricted |
4d
|
Expression domains failed to extend into the pericardia! cavity.
|
Specimen
4e:
(close )
|
Genetic Background:
|
involves: 129S/SvEv * 129S1/Sv * 129X1/SvJ
|
|
Mutant Allele(s):
|
Foxa2tm1Jrt/Foxa2+
Nodaltm1Rob/Nodal+
|
|
Age:
|
embryonic day 8.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS13: lateral plate mesoderm |
Present |
Regionally restricted |
4e
|
Expression domains failed to extend into the pericardia! cavity.
|
Specimen
not shown:
(close )
|
Genetic Background:
|
involves: 129S/SvEv * 129S1/Sv * 129X1/SvJ
|
|
Mutant Allele(s):
|
Foxa2tm1Jrt/Foxa2tm1Jrt
Nodaltm1Rob/Nodal+
|
|
Age:
|
embryonic day 8.5
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|
Sex:
|
Not Specified
|
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Type:
|
whole mount
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Analysis Tools