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Gene Expression Data
Assay Details
Assay
Reference: J:234927 Orquera DP, et al., Essential function of the transcription factor Rax in the early patterning of the mammalian hypothalamus. Dev Biol. 2016 Aug 1;416(1):212-24
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6392059
Gene symbol: Shh
Gene name: sonic hedgehog
Antibody: 5E1
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 7Q/S: embryonic day 8.5 (more )
Note: Triple labeled: green - Shh; red - Rax (assay MGI:6392060); blue - DAPI.
Structure Level Pattern Image Note
TS13: future prosencephalon Present Regionally restricted 7Q , 7S Expression domain at midline of rostral forebrain, corresponding to future ventral hypothalamus.
Specimen S3B/D/E: embryonic day 9.5 (more )
Note: Triple labeled: green - Shh; red - Rax (assay MGI:6392060); blue - DAPI.
Structure Level Pattern Image Note
TS15: diencephalon Present Regionally restricted S3E , S3B , S3D In dorsomedial terminal hypothalamus, co-expression with Rax at hypothalamic midline.
Specimen S3G/I/J: embryonic day 9.5 (more )
Note: Triple labeled: green - Shh; red - Rax (assay MGI:6392060); blue - DAPI.
Structure Level Pattern Image Note
TS15: diencephalon Present Regionally restricted S3J , S3I , S3G In dorsomedial terminal hypothalamus, co-expression with Rax at hypothalamic midline.
Specimen S3L/N/O: embryonic day 9.5 (more )
Note: Triple labeled: green - Shh; red - Rax (assay MGI:6392060); blue - DAPI.
Structure Level Pattern Image Note
TS15: diencephalon Weak Regionally restricted S3O , S3N , S3L In ventromedial terminal hypothalus, expression reduced at hypothalamic midline.
Specimen S3Q/S/T: embryonic day 9.5 (more )
Note: Triple labeled: green - Shh; red - Rax (assay MGI:6392060); blue - DAPI.
Structure Level Pattern Image Note
TS15: diencephalon Weak Regionally restricted S3Q , S3T , S3S In ventromedial terminal hypothalus, expression reduced at hypothalamic midline.
Specimen S4B/D/E: embryonic day 10.5 (more )
Note: Triple labeled: green - Shh; red - Rax (assay MGI:6392060); blue - DAPI.
Structure Level Pattern Image Note
TS17: diencephalon Present Regionally restricted S4E , S4B , S4D In dorsomedial terminal hypothalamus, co-expression with Rax at hypothalamic midline.
Specimen S4G/I/J: embryonic day 10.5 (more )
Note: Triple labeled: green - Shh; red - Rax (assay MGI:6392060); blue - DAPI.
Structure Level Pattern Image Note
TS17: diencephalon Present Regionally restricted S4J , S4G , S4I In dorsomedial terminal hypothalamus, co-expression with Rax at hypothalamic midline.
Specimen S4L/N/O: embryonic day 10.5 (more )
Note: Triple labeled: green - Shh; red - Rax (assay MGI:6392060); blue - DAPI.
Structure Level Pattern Image Note
TS17: diencephalon Present Regionally restricted S4N , S4O , S4L In ventromedial terminal hypothalus, expression absent from hypothalamic midline.
Specimen S4Q/S/T: embryonic day 10.5 (more )
Note: Triple labeled: green - Shh; red - Rax (assay MGI:6392060); blue - DAPI.
Structure Level Pattern Image Note
TS17: diencephalon Present Regionally restricted S4Q , S4T , S4S In ventromedial terminal hypothalus, expression absent from hypothalamic midline.
Specimen S4V/X/Y: embryonic day 10.5 (more )
Note: Triple labeled: green - Shh; red - Rax (assay MGI:6392060); blue - DAPI.
Structure Level Pattern Image Note
TS17: diencephalon Present Regionally restricted S4V , S4X , S4Y In ventromedial terminal hypothalus, expression absent from hypothalamic midline.
Specimen S5B/D/E: embryonic day 11.5 (more )
Note: Triple labeled: green - Shh; red - Rax (assay MGI:6392060); blue - DAPI.
Structure Level Pattern Image Note
TS19: diencephalon Present Regionally restricted S5D , S5E , S5B No longer co-expressed with Rax at dorsal levels.
Specimen S5G/I/J: embryonic day 11.5 (more )
Note: Triple labeled: green - Shh; red - Rax (assay MGI:6392060); blue - DAPI.
Structure Level Pattern Image Note
TS19: diencephalon Present Regionally restricted S5G , S5J , S5I No longer co-expressed with Rax at dorsal levels.
Specimen S5L/N/O: embryonic day 11.5 (more )
Note: Triple labeled: green - Shh; red - Rax (assay MGI:6392060); blue - DAPI.
Structure Level Pattern Image Note
TS19: diencephalon Present Regionally restricted S5O , S5N , S5L Co-expression with Rax limited to bilateral stripes in lateral wall of hypothalamus.
Specimen S5Q/S/T: embryonic day 11.5 (more )
Note: Triple labeled: green - Shh; red - Rax (assay MGI:6392060); blue - DAPI.
Structure Level Pattern Image Note
TS19: diencephalon Present Regionally restricted S5Q , S5T , S5S Co-expression with Rax limited to bilateral stripes in lateral wall of hypothalamus.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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