Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:282727
Li C, et al., Characterizing a novel vGlut3-P2A-iCreER knockin mouse strain in cochlea. Hear Res. 2018 Jul;364:12-24
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:6391229
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Gene symbol:
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Slc17a8
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Gene name:
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solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 8
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Probe:
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vGlut3 cDNA1
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
|
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Results
|
Specimen
not shown:
embryonic day 15.5
(more )
Specimen
not shown:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 15.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
1C:
embryonic day 18.0
(more )
Specimen
1C:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 18.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: utricle epithelium |
Strong |
Regionally restricted |
1D
,
1C
|
Expression is seen in hair cells.
|
TS26: saccule epithelium |
Strong |
Regionally restricted |
1C
|
Expression is seen in hair cells.
|
TS26: organ of Corti |
Weak |
Regionally restricted |
1F
,
1C
|
Expression is seen in basal and middle turn inner hair cells. It was never observed in outer hair cells at this age.
|
Specimen
2A:
postnatal day 3
(more )
Specimen
2A:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
postnatal day 3
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
2B:
postnatal day 3
(more )
Specimen
2B:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
postnatal day 3
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: organ of Corti |
Weak |
Regionally restricted |
2B
|
Expression is seen in the inner and outer hair cells.
|
Specimen
3A:
postnatal day 7
(more )
Specimen
3A:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
postnatal day 7
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: organ of Corti |
Strong |
Regionally restricted |
3A
|
Expression is seen in the inner hair cells in the basal turn. Expression is weak or undetectable in the outer hair cells.
|
Specimen
3B:
postnatal day 7
(more )
Specimen
3B:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
postnatal day 7
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: organ of Corti |
Strong |
Regionally restricted |
3B
|
Expression is seen in the inner hair cells in the middle turn. Expression is weak or undetectable in the outer hair cells.
|
Specimen
3C:
postnatal day 7
(more )
Specimen
3C:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
postnatal day 7
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: organ of Corti |
Strong |
Regionally restricted |
3C
|
Expression is seen in the inner hair cells in the apex. Expression is weak or undetectable in the outer hair cells.
|
Specimen
3D:
postnatal day 7
(more )
Specimen
3D:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
postnatal day 7
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: utricle epithelium |
Strong |
Regionally restricted |
3D
|
Expression is seen in hair cells.
|
Specimen
7A:
postnatal day 3
(more )
Specimen
7A:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
postnatal day 3
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
|