Gene Expression Data
Assay Details
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Assay
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Reference:
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J:261765
Knoll JG, et al., Developmental profile and sexually dimorphic expression of kiss1 and kiss1r in the fetal mouse brain. Front Endocrinol (Lausanne). 2013;4:140
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:6391014
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Gene symbol:
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Kiss1
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Gene name:
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KiSS-1 metastasis-suppressor
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Probe:
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Kiss1 cDNA1
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
1 E13:
embryonic day 13.5
(more  )
Specimen
1 E13:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 13.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Female
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Agarose
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: diencephalon |
Present |
Regionally restricted |
1_Kiss1 E13
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Expression was detected in a few cells near the median eminence.
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Specimen
1 E15:
embryonic day 15.5
(more )
Specimen
1 E15:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 15.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Female
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Agarose
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: diencephalon |
Present |
Regionally restricted |
1_Kiss1 E15
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The total number of expressing cells had greatly increased (from 30 cells/half brain to ~400 cells/half brain), although the distribution remained similar.
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Specimen
3A:
embryonic day 17.5
(more )
Specimen
3A:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 17.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Female
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Agarose
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Specimen
4 E13 Female:
embryonic day 13.5
(more )
Specimen
4 E13 Female:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 13.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Female
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Agarose
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: diencephalon |
Present |
Regionally restricted |
4_E13 Female
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Expression was detected in a few cells near the median eminence. There was no sex difference in the number of expressing cells.
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Specimen
4 E13 Male:
embryonic day 13.5
(more )
Specimen
4 E13 Male:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 13.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Agarose
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: diencephalon |
Present |
Regionally restricted |
4_E13 Male
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Expression was detected in a few cells near the median eminence. There was no sex difference in the number of expressing cells.
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Specimen
4 E17 Female:
embryonic day 17.5
(more )
Specimen
4 E17 Female:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 17.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Female
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Agarose
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Specimen
4 E17 Male:
embryonic day 17.5
(more )
Specimen
4 E17 Male:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 17.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Agarose
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Specimen
4 P12 Female:
postnatal day 12
(more )
Specimen
4 P12 Female:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal day 12
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Sex:
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Female
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Agarose
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: arcuate nucleus |
Present |
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4_P12 Female
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A transient sex difference in the number of labeled cells was evident at P12. More cells were labeled in the female compared with the male.
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Specimen
4 P12 Male:
postnatal day 12
(more )
Specimen
4 P12 Male:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal day 12
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Agarose
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: arcuate nucleus |
Present |
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4_P12 Male
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A transient sex difference in the number of labeled cells was evident at P12. More cells were labeled in the female compared with the male.
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Specimen
4 P35 Female:
postnatal day 35
(more )
Specimen
4 P35 Female:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal day 35
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Sex:
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Female
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Agarose
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Specimen
4 P35 Male:
postnatal day 35
(more )
Specimen
4 P35 Male:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal day 35
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Agarose
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Specimen
5 P12 Female:
postnatal day 12
(more )
Specimen
5 P12 Female:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal day 12
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Sex:
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Female
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Agarose
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: preoptic area |
Present |
Regionally restricted |
5_P12 Female
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Expression was detected in the rostral periventricular portion of the pre-optic area. There was not a statistically significant sex difference in the number of labeled cells at P12.
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Specimen
5 P12 Male:
postnatal day 12
(more )
Specimen
5 P12 Male:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal day 12
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Agarose
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: preoptic area |
Present |
Regionally restricted |
5_P12 Male
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Expression was detected in the rostral periventricular portion of the pre-optic area. There was not a statistically significant sex difference in the number of labeled cells at P12.
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Specimen
5 P35 Female:
postnatal day 35
(more )
Specimen
5 P35 Female:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal day 35
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Sex:
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Female
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Agarose
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: preoptic area |
Present |
Regionally restricted |
5_P35 Female
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Expression was detected in the rostral periventricular portion of the pre-optic area. At P35 there were dramatically more labeled cells compared to P12. In addition, there was a significant increase in the number of labeled cells in females compared to males.
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Specimen
5 P35 Male:
postnatal day 35
(more )
Specimen
5 P35 Male:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal day 35
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Agarose
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: preoptic area |
Present |
Regionally restricted |
5_P35 Male
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Expression was detected in the rostral periventricular portion of the pre-optic area. At P35 there were dramatically more labeled cells compared to P12. In addition, there was a significant increase in the number of labeled cells in females compared to males.
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Specimen
7A XX WT:
embryonic day 17.5
(more )
Specimen
7A XX WT:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Age:
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embryonic day 17.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Female
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Agarose
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Specimen
7A XY Sf1 KO:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Mutant Allele(s):
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Nr5a1tm1Klp/Nr5a1tm1Klp
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Age:
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embryonic day 17.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Agarose
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: hypothalamus periventricular zone |
Present |
Regionally restricted |
7A XY Sf1 KO
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Expression was present in the arcuate nucleus. Labeling was most comparable to sections from wild type XX mice by qualitative visual inspection of the optical density of reaction product.
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Specimen
7A XY WT:
embryonic day 17.5
(more )
Specimen
7A XY WT:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Age:
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embryonic day 17.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Agarose
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: hypothalamus periventricular zone |
Present |
Regionally restricted |
7A XY WT
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Expression was present in the arcuate nucleus. The labeled cells in XY wild type animals were consistently lighter in color than in either XX wild type or XY Sf1 KO mice.
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Analysis Tools