About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:272619 Aydogdu N, et al., TBX2 and TBX3 act downstream of canonical WNT signaling in patterning and differentiation of the mouse ureteric mesenchyme. Development. 2018 Nov 26;145(23):dev171827
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6385551
Gene symbol: Trp63
Gene name: transformation related protein 63
Antibody: Anti-deltaNp63 (619001)
Detection system: Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
Assay notes: The signal was amplified using the Tyramide Signal Amplification system, but the fluorophore used was not specified.
Results
Specimen 2D control left: embryonic day 18.5; Tbx2tm2.2Vmc/?, Tbx3tm3.2Moon/?, Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/Gt(ROSA)26Sor+ (more )
Note: Double labeled: red - Trp63; green - Krt5 (assay MGI:6385550).
Structure Level Pattern Image Note
TS26: basal cell layer of ureter Present 2D control left Expression was detected in urothelial basal cells, which were also stained with Krt5.
TS26: intermediate cell layer of ureter Present 2D control left
Specimen 2D Tbx18cre/+;Tbx2fl/fl;Tbx3fl/fl left: embryonic day 18.5; Tbx2tm2.2Vmc/Tbx2tm2.2Vmc, Tbx3tm3.2Moon/Tbx3tm3.2Moon, Tbx18tm4(cre)Akis/Tbx18+, Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Double labeled: red - Trp63; green - Krt5 (assay MGI:6385550).
Structure Level Pattern Image Note
TS26: basal cell layer of ureter Present 2D Tbx18cre/+;Tbx2fl/fl;Tbx3fl/fl left Normal expression was detected in urothelial basal cells of the conditional mutant. The layer was also stained with Krt5.
TS26: intermediate cell layer of ureter Present 2D Tbx18cre/+;Tbx2fl/fl;Tbx3fl/fl left Normal expression was detected in urothelial intermediate cells of the conditional mutant.
Specimen 2D control right: embryonic day 18.5; Tbx2tm2.2Vmc/?, Tbx3tm3.2Moon/?, Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/Gt(ROSA)26Sor+ (more )
Note: Double labeled: green - Trp63; red - Upk1b (assay MGI:6385555).
Structure Level Pattern Image Note
TS26: intermediate cell layer of ureter Present 2D control right Expression was detected in urothelial intermediate cells, which were also stained with Upk1b.
TS26: basal cell layer of ureter Present 2D control right
Specimen 2D Tbx18cre/+;Tbx2fl/fl;Tbx3fl/fl right: embryonic day 18.5; Tbx2tm2.2Vmc/Tbx2tm2.2Vmc, Tbx3tm3.2Moon/Tbx3tm3.2Moon, Tbx18tm4(cre)Akis/Tbx18+, Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Double labeled: green - Trp63; red - Upk1b (assay MGI:6385555).
Structure Level Pattern Image Note
TS26: intermediate cell layer of ureter Present 2D Tbx18cre/+;Tbx2fl/fl;Tbx3fl/fl right Normal expression was detected in urothelial intermediate cells of the conditional mutant. The layer was also stained with Upk1b.
TS26: basal cell layer of ureter Present 2D Tbx18cre/+;Tbx2fl/fl;Tbx3fl/fl right Normal expression was detected in urothelial basal cells of the conditional mutant.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
05/07/2024
MGI 6.23 		The Jackson Laboratory