Probe:	6494175
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS15: bulbus cordis	Strong		2A Lateral magnified , 2A Lateral
TS15: future forebrain	Strong		2A Lateral magnified , 2A Lateral
TS15: future hindbrain	Strong		2A Lateral magnified , 2A Lateral
TS15: future midbrain	Strong		2A Lateral magnified , 2A Lateral
TS15: midbrain-hindbrain junction	Strong		2A Lateral magnified , 2A Lateral
TS15: 1st branchial arch mandibular component	Present		2A Lateral magnified , 2A Lateral
TS15: fronto-nasal process	Present		2A Lateral magnified , 2A Lateral
TS15: trigeminal preganglion	Present		2A Lateral magnified , 2A Lateral
TS15: 1st branchial arch maxillary component	Present	Regionally restricted	2A Lateral , 2A Lateral magnified
TS15: forelimb bud	Present		2A Lateral
TS15: dorsal aorta	Strong		2A Lateral
TS15: nose	Present	Regionally restricted	2A Lateral , 2A Lateral magnified	Expression is in the nasal pit.
TS15: otocyst	Weak	Regionally restricted	2A Lateral magnified , 2A Lateral	Expression is in the rostral domain.
TS15: 2nd branchial arch	Present	Regionally restricted	2A Lateral , 2A Lateral magnified	Expression is in the proximal regions.
TS15: 3rd branchial arch	Present	Regionally restricted	2A Lateral , 2A Lateral magnified	Expression is in the proximal regions.
TS15: somite	Weak		2A Lateral
TS15: optic cup	Weak		2A Lateral , 2A Lateral magnified
TS15: aortic sac	Absent		2A Lateral magnified , 2A Lateral

Structure	Level	Pattern	Image	Note
TS15: future forebrain	Strong		2A Frontal
TS15: future hindbrain	Strong		2A Frontal
TS15: future midbrain	Strong		2A Frontal
TS15: midbrain-hindbrain junction	Strong		2A Frontal
TS15: forelimb bud	Present		2A Frontal
TS15: fronto-nasal process	Present		2A Frontal
TS15: heart	Present		2A Frontal

Structure	Level	Pattern	Image	Note
TS15: future forebrain	Strong		2A Dorsal
TS15: future hindbrain	Strong		2A Dorsal
TS15: future midbrain	Strong		2A Dorsal
TS15: midbrain-hindbrain junction	Strong		2A Dorsal
TS15: forelimb bud	Present		2A Dorsal

Structure	Level	Pattern	Image	Note
TS17: aortic sac	Strong		2B Lateral , 2B Lateral magnified
TS17: forebrain	Strong		2B Lateral , 2B Lateral magnified
TS17: heart	Strong		2B Lateral , 2B Lateral magnified
TS17: hindbrain	Strong		2B Lateral , 2B Lateral magnified
TS17: midbrain	Strong		2B Lateral , 2B Lateral magnified
TS17: neural tube	Strong		2B Lateral , 2B Lateral magnified
TS17: fronto-nasal process	Present		2B Lateral magnified , 2B Lateral
TS17: latero-nasal process	Present		2B Lateral magnified , 2B Lateral
TS17: nasal pit	Present		2B Lateral magnified , 2B Lateral
TS17: trigeminal ganglion	Present		2B Lateral magnified , 2B Lateral
TS17: 1st branchial arch	Present	Regionally restricted	2B Lateral , 2B Lateral magnified	Expression is in the proximal regions.
TS17: 2nd branchial arch	Present	Regionally restricted	2B Lateral , 2B Lateral magnified	Expression is in the proximal regions.
TS17: 3rd branchial arch	Present	Regionally restricted	2B Lateral , 2B Lateral magnified	Expression is in the proximal regions.
TS17: 4th branchial arch	Present	Regionally restricted	2B Lateral , 2B Lateral magnified	Expression is in the proximal regions.
TS17: dorsal aorta	Present		2B Lateral
TS17: forelimb bud	Present		2B Lateral
TS17: midbrain-hindbrain junction	Present		2B Lateral
TS17: otocyst	Present	Regionally restricted	2B Lateral , 2B Lateral magnified	Expression is in the rostral domain.

Structure	Level	Pattern	Image	Note
TS17: forebrain	Present		2B Frontal
TS17: heart	Present		2B Frontal
TS17: midbrain	Present		2B Frontal
TS17: midbrain-hindbrain junction	Present		2B Frontal
TS17: neural tube	Present		2B Frontal

Structure	Level	Pattern	Image	Note
TS17: forelimb bud	Present		2B Dorsal
TS17: midbrain	Present		2B Dorsal
TS17: neural tube	Present		2B Dorsal

Structure	Level	Pattern	Image	Note
TS20: diencephalon	Strong		3A
TS20: brain	Present		3A
TS20: facial ganglion	Present		3A

Structure	Level	Pattern	Image	Note
TS20: diencephalon	Strong		3B
TS20: trigeminal ganglion	Strong		3B
TS20: brain	Present		3B
TS20: facial ganglion	Present		3B

Structure	Level	Pattern	Image	Note
TS20: diencephalon	Strong		3C
TS20: trigeminal ganglion	Strong		3C
TS20: brain	Present		3C
TS20: facial ganglion	Present		3C

Structure	Level	Pattern	Image	Note
TS20: oral epithelium	Present		3D
TS20: palatal shelf epithelium	Present		3D
TS20: tongue epithelium	Present		3D

Structure	Level	Pattern	Image	Note
TS20: oral epithelium	Present		3E
TS20: dental placode	Present	Regionally restricted	3E	Expression is in the early dental lamina of the upper jaw.
TS20: tooth bud	Present	Regionally restricted	3E	Expression is in the early dental lamina of the upper jaw.

Structure	Level	Pattern	Image	Note
TS20: ventricle cardiac muscle	Present		3F
TS20: ventricle endocardial lining	Present		3F

Structure	Level	Pattern	Image	Note
TS21: brain	Strong		3G

Structure	Level	Pattern	Image	Note
TS21: brain	Strong		3H

Structure	Level	Pattern	Image	Note
TS21: brain	Strong		3I
TS21: trigeminal ganglion	Strong		3I

Structure	Level	Pattern	Image	Note
TS21: oral epithelium	Present		3J
TS21: palatal shelf epithelium	Present		3J
TS21: salivary gland epithelium	Present		3J
TS21: tongue epithelium	Present		3J

Structure	Level	Pattern	Image	Note
TS21: dental lamina	Present		3K
TS21: tooth bud	Present		3K
TS21: tooth mesenchyme	Present	Regionally restricted	3K	Expression is in the ectomesenchymal dental papilla.

Structure	Level	Pattern	Image	Note
TS21: ventricle cardiac muscle	Present		3L
TS21: ventricle endocardial lining	Present		3L

Structure	Level	Pattern	Image	Note
TS22: brain	Present		4A
TS22: cerebral cortex	Present		4A
TS22: diencephalon	Present		4A
TS22: trigeminal ganglion	Present		4A

Structure	Level	Pattern	Image	Note
TS22: brain	Present		4B
TS22: cerebral cortex	Present		4B
TS22: diencephalon	Present		4B
TS22: trigeminal ganglion	Present		4B

Structure	Level	Pattern	Image	Note
TS22: brain	Present		4C
TS22: cerebral cortex	Present		4C
TS22: diencephalon	Present		4C

Structure	Level	Pattern	Image	Note
TS22: palatal shelf epithelium	Present	Regionally restricted	4D	Expression is in the medial edge epithelium.
TS22: tongue epithelium	Present		4D
TS22: oral epithelium	Present	Regionally restricted	4D	Expression is in the floor of the oral cavity.

Structure	Level	Pattern	Image	Note
TS22: trigeminal ganglion	Present		4E

Structure	Level	Pattern	Image	Note
TS22: cap stage tooth	Present	Diffuse	4F
TS22: dental lamina	Present	Diffuse	4F
TS22: dental papilla	Present	Diffuse	4F
TS22: tooth epithelium	Present	Diffuse	4F

Structure	Level	Pattern	Image	Note
TS22: salivary gland epithelium	Present	Regionally restricted	4G	Expression is in the branching epithelium.

Structure	Level	Pattern	Image	Note
TS22: endocardial lining	Strong		4H
TS22: atrium cardiac muscle	Present		4H
TS22: interventricular septum muscular part	Present		4H
TS22: ventricle cardiac muscle	Present		4H

Structure	Level	Pattern	Image	Note
TS23: cerebral cortex	Strong		4I
TS23: diencephalon	Strong		4I
TS23: brain	Present		4I

Structure	Level	Pattern	Image	Note
TS23: cerebral cortex	Strong		4J
TS23: diencephalon	Strong		4J
TS23: brain	Present		4J

Structure	Level	Pattern	Image	Note
TS23: cerebral cortex	Strong		4K
TS23: diencephalon	Strong		4K
TS23: brain	Present		4K

Structure	Level	Pattern	Image	Note
TS23: tongue mesenchyme	Present		4L
TS23: oral epithelium	Present	Regionally restricted	4L	Expression is in the floor of the oral cavity.

Structure	Level	Pattern	Image	Note
TS23: lower jaw incisor	Strong		4M

Structure	Level	Pattern	Image	Note
TS23: cap stage tooth	Present	Diffuse	4N
TS23: dental lamina	Present	Diffuse	4N
TS23: dental papilla	Present	Diffuse	4N
TS23: tooth epithelium	Present	Diffuse	4N

Structure	Level	Pattern	Image	Note
TS23: salivary gland	Present	Regionally restricted	4O	Expression is in the basal cytoplasm and secretory vesicles of the serous acini and in the interlobular excretory ducts.

Structure	Level	Pattern	Image	Note
TS23: endocardial lining	Strong		4P
TS23: atrium cardiac muscle	Present		4P
TS23: interventricular septum muscular part	Present		4P
TS23: ventricle cardiac muscle	Present		4P

Structure	Level	Pattern	Image	Note
TS24: main olfactory bulb	Present	Regionally restricted	4Q	Expression is in cells lining the walls of the olfactory bulbs bilaterally.
TS24: tongue epithelium	Present		4Q
TS24: oral epithelium	Present	Regionally restricted	4Q	Expression is in the floor of the oral cavity.

Structure	Level	Pattern	Image	Note
TS24: bell stage tooth	Present		4R
TS24: dental lamina	Present		4R
TS24: dental papilla	Present		4R
TS24: enamel knot	Present		4R
TS24: inner enamel epithelium	Present		4R
TS24: outer enamel epithelium	Present		4R

Structure	Level	Pattern	Image	Note
TS24: salivary gland	Present	Regionally restricted	4S	Expression is in the basal cytoplasm and secretory vesicles of the serous acini and in the interlobular excretory ducts.

Structure	Level	Pattern	Image	Note
TS22: lens anterior epithelium	Present		5A
TS22: neural retina	Present		5A
TS22: cornea epithelium	Present	Regionally restricted	5A	Expression is in the anterior and posterior corneal epithelium.

Structure	Level	Pattern	Image	Note
TS24: lens fiber	Present	Regionally restricted	5B	Expression is in the medial primary fiber cells and proximal secondary fiber cells.
TS24: pigmented retinal epithelium	Present		5B
TS24: retina inner nuclear layer	Present		5B
TS24: retina outer nuclear layer	Present		5B
TS24: neural retina	Present	Regionally restricted	5B	Expression is in the inner plexiform layer.

Structure	Level	Pattern	Image	Note
TS21: adenohypophysis	Present		5C
TS21: adenohypophysis pars intermedia	Present		5C
TS21: neurohypophysis	Present		5C

Structure	Level	Pattern	Image	Note
TS23: adenohypophysis	Present		5D
TS23: adenohypophysis pars intermedia	Present		5D
TS23: neurohypophysis	Present		5D

Structure	Level	Pattern	Image	Note
TS23: facial muscle	Present	Regionally restricted	5E	Expression is in the connective tissue of the lateral pterygoid muscle.

Structure	Level	Pattern	Image	Note
TS23: pericardium	Present		5F

Structure	Level	Pattern	Image	Note
TS23: genioglossus	Present		5G
TS23: hyoglossus	Present		5G
TS23: mylohyoid	Present		5G
TS23: tongue extrinsic muscle	Present		5G
TS23: tongue intrinsic muscle	Present		5G
TS23: pharynx skeletal muscle	Present	Regionally restricted	5G	Expression is in the geniohyoid muscle.

Structure	Level	Pattern	Image	Note
TS24: genioglossus	Present		5H
TS24: hyoglossus	Present		5H
TS24: mylohyoid	Present		5H
TS24: tongue extrinsic muscle	Present		5H
TS24: tongue intrinsic muscle	Present		5H
TS24: pharynx skeletal muscle	Present	Regionally restricted	5H	Expression is in the geniohyoid muscle.

Structure	Level	Pattern	Image	Note
TS20: spinal cord mantle layer	Present	Regionally restricted	5I	Expression is in the middle of the caudal neural tube.
TS20: dorsal root ganglion	Present		5I
TS20: spinal cord marginal layer	Absent		5I

Structure	Level	Pattern	Image	Note
TS21: dorsal root ganglion	Present		5J
TS21: spinal cord mantle layer	Present		5J
TS21: spinal cord ventricular layer	Present		5J
TS21: spinal cord marginal layer	Absent		5J

Structure	Level	Pattern	Image	Note
TS22: alar columns	Present		5K
TS22: basal columns	Present		5K
TS22: dorsal root ganglion	Present		5K
TS22: spinal cord mantle layer	Present		5K
TS22: spinal cord ventricular layer	Present		5K
TS22: spinal cord marginal layer	Absent		5K

Structure	Level	Pattern	Image	Note
TS23: alar columns	Present	Regionally restricted	5L	Expression is in part of the middle mantle zone.
TS23: basal columns	Present	Regionally restricted	5L	Expression is in part of the middle mantle zone.
TS23: spinal cord mantle layer	Present	Regionally restricted	5L	Expression is in part of the middle mantle zone.
TS23: dorsal root ganglion	Present		5L
TS23: spinal cord marginal layer	Absent		5L

Structure	Level	Pattern	Image	Note
TS20: pretubular aggregate	Present		5M , 5M'
TS20: mesenchyme of metanephros	Present	Regionally restricted	5M , 5M'	Expression is in the condensing mesenchyme.
TS20: early nephron	Present	Regionally restricted	5M' , 5M	Expression is in the metanephric glomerulus in the uretric bud.
TS20: sympathetic ganglion	Strong	Regionally restricted	5M	Expression is in the preaortic abdominal sympathetic ganglia.

Structure	Level	Pattern	Image	Note
TS21: pretubular aggregate	Present		5N' , 5N
TS21: mesenchyme of metanephros	Present	Regionally restricted	5N' , 5N	Expression is in the condensing mesenchyme.
TS21: early nephron	Present	Regionally restricted	5N , 5N'	Expression is in the metanephric glomerulus in the uretric bud.

Structure	Level	Pattern	Image	Note
TS22: pretubular aggregate	Present		5O , 5O'
TS22: mesenchyme of metanephros	Present	Regionally restricted	5O , 5O'	Expression is in the condensing mesenchyme.
TS22: maturing glomerular tuft	Present	Regionally restricted	5O , 5O'	Expression is in the metanephric glomerulus in the uretric bud.
TS22: sympathetic ganglion	Strong	Regionally restricted	5O	Expression is in the preaortic abdominal sympathetic ganglia.

Structure	Level	Pattern	Image	Note
TS23: pretubular aggregate	Strong		5P , 5P'
TS23: mesenchyme of metanephros	Strong	Regionally restricted	5P , 5P'	Expression is in the condensing mesenchyme.
TS23: maturing glomerular tuft	Strong	Regionally restricted	5P , 5P'	Expression is in the metanephric glomerulus in the uretric bud.