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Gene Expression Data
Assay Details
Assay
Reference: J:236376 Zheng D, et al., Pou4f2-GFP knock-in mouse line: A model for studying retinal ganglion cell development. Genesis. 2016 Oct;54(10):534-541
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:6384915
Gene symbol: Pou4f2
Gene name: POU domain, class 4, transcription factor 2
Reporter: GFP
Antibody: anti-GFP (ab13970)
Results
Specimen 2a: embryonic day 13.5; Pou4f2tm1Ygwg/Pou4f2+ (more )
Note: Double labeled: green - Pou4f2 reporter; red - Pou4f2 protein (assay MGI:6384905).
Structure Level Pattern Image Note
TS21: neural retina Present Regionally restricted 2c , 2a Expression is in the retinal ganglion cells.
Specimen 2a': postnatal day 0; Pou4f2tm1Ygwg/Pou4f2+ (more )
Note: Double labeled: green - Pou4f2 reporter; red - Pou4f2 protein (assay MGI:6384905).
Structure Level Pattern Image Note
TS27: retina ganglion cell layer Present 2c' , 2a'
Specimen 3a': embryonic day 11.5; Pou4f2tm1Ygwg/Pou4f2tm1Ygwg (more )
Structure Level Pattern Image Note
TS19: neural retina Present Regionally restricted 3a' Expression is in a few migrating cells at the center.
Specimen 3b': embryonic day 12.5; Pou4f2tm1Ygwg/Pou4f2tm1Ygwg (more )
Structure Level Pattern Image Note
TS20: neural retina Present Regionally restricted 3b' Expression is in the retinal ganglion cells.
Specimen 3c': embryonic day 13.5; Pou4f2tm1Ygwg/Pou4f2tm1Ygwg (more )
Structure Level Pattern Image Note
TS21: neural retina Present Regionally restricted 3c' Expression is in the retinal ganglion cells.
Specimen 3d': embryonic day 15.5; Pou4f2tm1Ygwg/Pou4f2tm1Ygwg (more )
Structure Level Pattern Image Note
TS23: retina layer Present Regionally restricted 3d' Expression is in the retinal ganglion cells.
Specimen 3e': embryonic day 17.5; Pou4f2tm1Ygwg/Pou4f2tm1Ygwg (more )
Structure Level Pattern Image Note
TS25: retina layer Present Regionally restricted 3e' Expression is in the retinal ganglion cells.
Specimen 3f': postnatal day 0; Pou4f2tm1Ygwg/Pou4f2tm1Ygwg (more )
Structure Level Pattern Image Note
TS27: retina ganglion cell layer Present 3f'
Specimen 3g': postnatal day 28; Pou4f2tm1Ygwg/Pou4f2tm1Ygwg (more )
Structure Level Pattern Image Note
TS28: retina ganglion cell layer Weak 3g'
Specimen 5a': embryonic day 12.5; Pou4f2tm1Ygwg/Pou4f2tm1Ygwg (more )
Note: Double labeled: green - Pou4f2 reporter; red - Isl1 (assay MGI:6384912).
Structure Level Pattern Image Note
TS20: neural retina Present Regionally restricted 5a' Expression is in the retinal ganglion cells.
Specimen 5b': embryonic day 13.5; Pou4f2tm1Ygwg/Pou4f2tm1Ygwg (more )
Note: Double labeled: green - Pou4f2 reporter; red - Isl1 (assay MGI:6384912).
Structure Level Pattern Image Note
TS21: neural retina Present Regionally restricted 5b' Expression is in the retinal ganglion cells.
Specimen 6a: embryonic day 14.5; Pou4f2tm1Ygwg/Pou4f2tm1Ygwg (more )
Structure Level Pattern Image Note
TS22: neural retina Present Regionally restricted 6a Expression is in the retinal ganglion cells.
Specimen 6b: embryonic day 16.5; Pou4f2tm1Ygwg/Pou4f2tm1Ygwg (more )
Structure Level Pattern Image Note
TS24: retina layer Present Regionally restricted 6b Expression is in the retinal ganglion cells.
Specimen S1a: embryonic day 11.5; Pou4f2tm1Ygwg/Pou4f2+ (more )
Note: Double labeled: green - Pou4f2 reporter; red - Pou4f2 protein (assay MGI:6384905).
Structure Level Pattern Image Note
TS19: brain Present S1a , S1c
Specimen S1a': embryonic day 13.5; Pou4f2tm1Ygwg/Pou4f2+ (more )
Note: Double labeled: green - Pou4f2 reporter; red - Pou4f2 protein (assay MGI:6384905).
Structure Level Pattern Image Note
TS21: brain Present S1a' , S1c'
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory