Gene Expression Data
Assay Details
Assay
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Reference:
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J:236376
Zheng D, et al., Pou4f2-GFP knock-in mouse line: A model for studying retinal ganglion cell development. Genesis. 2016 Oct;54(10):534-541
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6384905
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Gene symbol:
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Pou4f2
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Gene name:
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POU domain, class 4, transcription factor 2
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Results
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Specimen
2b:
embryonic day 13.5; Pou4f2tm1Ygwg/Pou4f2 +
(more )
Note: Double labeled: red - Pou4f2 protein; green - Pou4f2 reporter (assay MGI:6384915).
Specimen
2b:
(close )
Genetic Background:
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involves: 129S6/SvEvTac * C57BL/6J
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Mutant Allele(s):
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Pou4f2tm1Ygwg/Pou4f2+
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Pou4f2 protein; green - Pou4f2 reporter (assay MGI:6384915).
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: neural retina |
Present |
Regionally restricted |
2c
,
2b
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Expression is in the retinal ganglion cells.
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Specimen
2b':
postnatal day 0; Pou4f2tm1Ygwg/Pou4f2 +
(more )
Note: Double labeled: red - Pou4f2 protein; green - Pou4f2 reporter (assay MGI:6384915).
Specimen
2b':
(close )
Genetic Background:
|
involves: 129S6/SvEvTac * C57BL/6J
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Mutant Allele(s):
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Pou4f2tm1Ygwg/Pou4f2+
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Age:
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postnatal day 0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Pou4f2 protein; green - Pou4f2 reporter (assay MGI:6384915).
|
Specimen
3a:
embryonic day 11.5
(more )
Specimen
3a:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
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Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: neural retina |
Present |
Regionally restricted |
3a
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Expression is in a few migrating cells in the center.
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Specimen
3b:
embryonic day 12.5
(more )
Specimen
3b:
(close )
Genetic Background:
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Not Specified
|
Age:
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embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: neural retina |
Present |
Regionally restricted |
3b
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Expression is in the retinal ganglion cells.
|
Specimen
3c:
embryonic day 13.5
(more )
Specimen
3c:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
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section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: neural retina |
Present |
Regionally restricted |
3c
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Expression is in the retinal ganglion cells.
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Specimen
3d:
embryonic day 15.5
(more )
Specimen
3d:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS23: retina layer |
Present |
Regionally restricted |
3d
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Expression is in the retinal ganglion cells.
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Specimen
3e:
embryonic day 17.5
(more )
Specimen
3e:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 17.5
|
Sex:
|
Not Specified
|
Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS25: retina layer |
Present |
Regionally restricted |
3e
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Expression is in the retinal ganglion cells.
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Specimen
3f:
postnatal day 0
(more )
Specimen
3f:
(close )
Genetic Background:
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Not Specified
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Age:
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postnatal day 0
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Sex:
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Not Specified
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Type:
|
section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
3g:
postnatal day 28
(more )
Specimen
3g:
(close )
Genetic Background:
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Not Specified
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Age:
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postnatal day 28
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
5a:
embryonic day 12.5
(more )
Note: Double labeled: green - Pou4f2; red - Isl1 (assay MGI:6384912).
Specimen
5a:
(close )
Genetic Background:
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Not Specified
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Pou4f2; red - Isl1 (assay MGI:6384912).
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: neural retina |
Present |
Regionally restricted |
5a
|
Expression is in the retinal ganglion cells.
|
Specimen
5b:
embryonic day 13.5
(more )
Note: Double labeled: green - Pou4f2; red - Isl1 (assay MGI:6384912).
Specimen
5b:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Pou4f2; red - Isl1 (assay MGI:6384912).
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: neural retina |
Present |
Regionally restricted |
5b
|
Expression is in the retinal ganglion cells.
|
Specimen
S1b:
embryonic day 11.5; Pou4f2tm1Ygwg/Pou4f2 +
(more )
Note: Double labeled: red - Pou4f2 protein; green - Pou4f2 reporter (assay MGI:6384915).
Specimen
S1b:
(close )
Genetic Background:
|
involves: 129S6/SvEvTac * C57BL/6J
|
Mutant Allele(s):
|
Pou4f2tm1Ygwg/Pou4f2+
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Pou4f2 protein; green - Pou4f2 reporter (assay MGI:6384915).
|
Specimen
S1b':
embryonic day 13.5; Pou4f2tm1Ygwg/Pou4f2 +
(more )
Note: Double labeled: red - Pou4f2 protein; green - Pou4f2 reporter (assay MGI:6384915).
Specimen
S1b':
(close )
Genetic Background:
|
involves: 129S6/SvEvTac * C57BL/6J
|
Mutant Allele(s):
|
Pou4f2tm1Ygwg/Pou4f2+
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Pou4f2 protein; green - Pou4f2 reporter (assay MGI:6384915).
|
|