About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:272586 Suzuki T, et al., Postnatal liver functional maturation requires Cnot complex-mediated decay of mRNAs encoding cell cycle and immature liver genes. Development. 2019 Feb 15;146(4):dev168146
Assay type: RT-PCR
MGI Accession ID: MGI:6383204
Gene symbol: Pcca
Gene name: propionyl-Coenzyme A carboxylase, alpha polypeptide
Probe: Pcca-pA, Pcca-pB
Assay notes: Quantitative RT-PCR. Gapdh mRNA levels were used for normalization. Expression levels of genes in 1 week control mice were set to 1.
Results
Image: 6B Pcca ©

 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified (a) Amount Genetic BackgroundMutant Allele(s)Sex Note
1w Control P w 1 TS28: liver Present 1 µg; total RNA involves: 129P2/OlaHsd Not Specified The following genotypes could have been used as control samples: (flox/+), (flox/flox), Alb-cre;(+/+) or Alb-cre;(flox/+).
1w Cnot3LKO P w 1 TS28: liver Present (b) 1 µg; total RNA involves: 129P2/OlaHsd * C57BL/6 * DBA Cnot3tm1.2Tya/Cnot3tm1.2Tya, Speer6-ps1Tg(Alb-cre)21Mgn/0Not Specified Conditional mutant.
4w Control P w 4 TS28: liver Present 1 µg; total RNA involves: 129P2/OlaHsd Not Specified The following genotypes could have been used as control samples: (flox/+), (flox/flox), Alb-cre;(+/+) or Alb-cre;(flox/+).
4w Cnot3LKO P w 4 TS28: liver Present (b) 1 µg; total RNA involves: 129P2/OlaHsd * C57BL/6 * DBA Cnot3tm1.2Tya/Cnot3tm1.2Tya, Speer6-ps1Tg(Alb-cre)21Mgn/0Not Specified Conditional mutant.
Notes:
(a) Expression increased from postnatal week 1 to postnatal week 4, even in the absence of Cnot3.
(b) Transcript level was significantly less in the mutant liver compared to control.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
04/16/2024
MGI 6.23 		The Jackson Laboratory