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Gene Expression Data
Assay Details
Assay
Reference: J:272586 Suzuki T, et al., Postnatal liver functional maturation requires Cnot complex-mediated decay of mRNAs encoding cell cycle and immature liver genes. Development. 2019 Feb 15;146(4):dev168146
Assay type: RT-PCR
MGI Accession ID: MGI:6383140
Gene symbol: Cdc25a
Gene name: cell division cycle 25A
Probe: Cdc25a-pA, Cdc25a-pB
Assay notes: Quantitative RT-PCR. Gapdh mRNA levels were used for normalization. Expression levels of genes in postnatal week 4 control mice were set to 1.
Results
 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified (a) Amount Genetic BackgroundMutant Allele(s)Sex Note
1w Control P w 1 TS28: liver Present 1 µg; total RNA involves: 129P2/OlaHsd Not Specified The following genotypes could have been used as control samples: (flox/+), (flox/flox), Alb-cre;(+/+) or Alb-cre;(flox/+).
1w Cnot3LKO P w 1 TS28: liver Present 1 µg; total RNA involves: 129P2/OlaHsd * C57BL/6 * DBA Cnot3tm1.2Tya/Cnot3tm1.2Tya, Speer6-ps1Tg(Alb-cre)21Mgn/0Not Specified Conditional mutant.
4w Control P w 4 TS28: liver Present 1 µg; total RNA involves: 129P2/OlaHsd Not Specified The following genotypes could have been used as control samples: (flox/+), (flox/flox), Alb-cre;(+/+) or Alb-cre;(flox/+).
4w Cnot3LKO P w 4 TS28: liver Present 1 µg; total RNA involves: 129P2/OlaHsd * C57BL/6 * DBA Cnot3tm1.2Tya/Cnot3tm1.2Tya, Speer6-ps1Tg(Alb-cre)21Mgn/0Not Specified Conditional mutant.
Notes:
(a) Transcripts were expressed more highly in the conditional mutant liver compared with control, mainly at 4 weeks of age. The results also showed that transcript level decreased less efficiently from postnatal week 1 to postnatal week 4 in the conditional mutant liver compared to control.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/16/2024
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