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Gene Expression Data
Assay Details
Assay
Reference: J:134435 Anselme I, et al., Defects in brain patterning and head morphogenesis in the mouse mutant Fused toes. Dev Biol. 2007 Apr 1;304(1):208-20
Assay type: RNA in situ
MGI Accession ID: MGI:6382583
Gene symbol: En1
Gene name: engrailed 1
Probe: En1 probe4
Probe preparation: Antisense labelled with digoxigenin RNA
Visualized with: Alkaline phosphatase
Results
Specimen 2D: embryonic day 8.0 (more )
Note: 6 somites. Also stained for Six3 (assay MGI:6382582) and Hnf1b (Vhnf1; assay MGI:6382584); all purple.
Structure Level Pattern Image Note
TS12: future brain Present Regionally restricted 2D Expression in broad domain centered on MHB.
TS12: midbrain-hindbrain junction Strong 2D High levels of expression at midbrain-hindbrain boundary (MHB).
TS12: future hindbrain Present Graded 2D Decreasing gradient anterior and posterior to MHB. Expression extends more caudally than that of Wnt1.
Specimen not shown: embryonic day 9.5 (more )
Note: 22 somites.
Structure Level Pattern Image Note
TS15: midbrain-hindbrain junction Present Regionally restricted Expression restricted to a ring at the midbrain-hindbrain boundary.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory