Gene Expression Data
Assay Details
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Assay
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Reference:
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J:194130
Appelbe OK, et al., Conditional deletions refine the embryonic requirement for Dlk1. Mech Dev. 2013 Feb;130(2-3):143-59
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6377442
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Gene symbol:
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Dlk1
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Gene name:
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delta like non-canonical Notch ligand 1
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Antibody:
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Anti-DLK (H-118; sc-25437)
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Detection system:
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Secondary antibody coupled to Alexa Fluor 647
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Assay notes:
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Dlk1 is an imprinted gene; it is expressed only from the paternally inherited allele.
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Results
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Specimen
6F/H:
postnatal adult; Tg(Ins2-cre)25Mgn/0
(more  )
Note: Control (rIP-Cre/+)
Specimen
6F/H:
(close  )
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Genetic Background:
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B6.Cg-Tg(Ins2-cre)25Mgn/J
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Mutant Allele(s):
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Tg(Ins2-cre)25Mgn/0
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Control (rIP-Cre/+)
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: pancreas |
Present |
Regionally restricted |
6H
,
6F
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Low, but detectable expression was observed in islet cells. High levels of expression were seen in the acinar cells surrounding the islets.
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Specimen
6G/I:
postnatal adult; Dlk1tm1.1Jvs/Dlk1 +, Tg(Ins2-cre)25Mgn/0
(more )
Note: Conditional mutant. Paternally inherited Dlk1Ins-pat allele.
Specimen
6G/I:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6 * DBA * FVB/N
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Mutant Allele(s):
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Dlk1tm1.1Jvs/Dlk1+
Tg(Ins2-cre)25Mgn/0
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Conditional mutant. Paternally inherited Dlk1Ins-pat allele.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: pancreas |
Weak |
Regionally restricted |
6G
,
6I
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The Dlk1<Ins-pat> specimen showed a virtually complete loss of expression in the islets, and a significant decrease in acinar cell expression.
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Specimen
7F/H:
postnatal adult; Tg(Gh1-cre)bKnmn/0
(more )
Note: Control (rGh-Cre/+)
Specimen
7F/H:
(close )
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Genetic Background:
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involves: C57BL/6 * FVB
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Mutant Allele(s):
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Tg(Gh1-cre)bKnmn/0
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Control (rGh-Cre/+)
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: adenohypophysis |
Present |
Regionally restricted |
7F
,
7H
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Expression was detected in GH-producing somatotrophs of the mature pituitary.
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Specimen
7G/I:
postnatal adult; Dlk1tm1.1Jvs/Dlk1 +, Tg(Gh1-cre)bKnmn/0
(more )
Note: Conditional mutant. Paternally inherited Dlk1Gh-pat allele.
Specimen
7G/I:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6 * FVB/N
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Mutant Allele(s):
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Dlk1tm1.1Jvs/Dlk1+
Tg(Gh1-cre)bKnmn/0
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Conditional mutant. Paternally inherited Dlk1Gh-pat allele.
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Specimen
8C/E:
embryonic day 12.5; Tg(Tie1-cre)9Ref/0
(more )
Note: Control (Tie1-Cre/+)
Specimen
8C/E:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Tg(Tie1-cre)9Ref/0
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Control (Tie1-Cre/+)
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: placenta |
Present |
Regionally restricted |
8E
,
8C
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Expression was detected in the placental endothelium.
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Specimen
8D/F:
embryonic day 12.5; Dlk1tm1.1Jvs/Dlk1 +, Tg(Tie1-cre)9Ref/0
(more )
Note: Conditional mutant. Paternally inherited Dlk1Tie1-pat allele.
Specimen
8D/F:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * 129S1/Sv * 129X1/SvJ * C57BL/6 * FVB/N
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Mutant Allele(s):
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Dlk1tm1.1Jvs/Dlk1+
Tg(Tie1-cre)9Ref/0
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Conditional mutant. Paternally inherited Dlk1Tie1-pat allele.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: placenta |
Trace |
Regionally restricted |
8F
,
8D
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Expression was significantly reduced in placental endothelial cells.
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