Probe:	Uncx4.1 probe14
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS17: somite	Present	Regionally restricted	6B	Expression in posterior compartment of somite,

Structure	Level	Pattern	Image	Note
TS17: somite	Present	Regionally restricted	6C	Expression in posterior compartment of somite, frequently irregular along the rostro-caudal axis of embryo.

Structure	Level	Pattern	Image	Note
TS19: somite	Present	Regionally restricted	6D	Expression in posterior compartment of somite,

Structure	Level	Pattern	Image	Note
TS19: somite	Present	Regionally restricted	6E	Expression in posterior compartment of somite,

Structure	Level	Pattern	Image	Note
TS19: somite	Present	Regionally restricted	6F	Authors report abnormal intrusion of expressing cells into anterior compartment, especially in dorsal somites, and stripes of expression partially close to each other in thoracic region.

Structure	Level	Pattern	Image	Note
TS19: somite	Present	Regionally restricted	6G	Authors report abnormal intrusion of expressing cells into anterior compartment, especially in dorsal somites.
TS19: tail somite	Present	Regionally restricted	6G	Stripes of expression continuous in dorsal region in curved parts of tail region.

Structure	Level	Pattern	Image	Note
TS19: tail somite	Present	Regionally restricted	S2A	Expression in posterior compartment of somite,

Structure	Level	Pattern	Image	Note
TS19: somite	Present	Regionally restricted	S2C	Authors report abnormal intrusion of expressing cells into anterior compartment, especially in dorsal somites.
TS19: tail somite	Present	Regionally restricted	S2C	Stripes of expression continuous in dorsal region in curved parts of tail region.

Structure	Level	Pattern	Image	Note
TS20: somite	Present	Regionally restricted	6H	Expression in posterior compartment of somite.

Structure	Level	Pattern	Image	Note
TS20: somite	Present	Regionally restricted	6I	Authors report abnormal intrusion of expressing cells into anterior compartment, especially in dorsal somites.
TS20: tail somite	Present	Regionally restricted	6I	Stripes of expression continuous in dorsal region in curved parts of tail region.

Structure	Level	Pattern	Image	Note
TS16: somite	Present		6L wt

Structure	Level	Pattern	Image	Note
TS16: somite	Present	Regionally restricted	6L Bn/y	Expressing and non-expressing stripes were thinner in 6 of 14 embryos Bn/Y or Bn/Bn embryos.

Structure	Level	Pattern	Image	Note
TS16: somite	Present	Regionally restricted	6L Bn/Bn	Expressing and non-expressing stripes were thinner in 6 of 14 embryos Bn/Y or Bn/Bn embryos.

Structure	Level	Pattern	Image	Note
TS16: somite	Present		6M

Structure	Level	Pattern	Image	Note
TS16: somite	Present	Regionally restricted	6N	Expressing and non-expressing stripes were thinner in 6 of 14 embryos Bn/Y or Bn/Bn embryos.

Structure	Level	Pattern	Image	Note
TS16: somite	Present	Regionally restricted	6O	Expressing and non-expressing stripes were thinner in 6 of 14 embryos Bn/Y or Bn/Bn embryos.

Structure	Level	Pattern	Image	Note
TS19: somite	Present		6P

Structure	Level	Pattern	Image	Note
TS19: somite	Present	Regionally restricted	6Q	Irregular stripe pattern was rare but seen in 1/6 Bn/Y embryos.

Structure	Level	Pattern	Image	Note
TS17: somite	Present		6R

Structure	Level	Pattern	Image	Note
TS17: somite	Present		6S

Structure	Level	Pattern	Image	Note
TS19: tail somite	Present		S3C

Structure	Level	Pattern	Image	Note
TS19: tail somite	Present	Regionally restricted	S3F	Authors report correct stripe of expression in newly formed somites, indicating anterior-posterior somite polarity normally specified.

Structure	Level	Pattern	Image	Note
TS13: somite	Present		7A

Structure	Level	Pattern	Image	Note
TS13: somite	Present		7B

Structure	Level	Pattern	Image	Note
TS13: somite	Present		7C

Structure	Level	Pattern	Image	Note
TS15: somite	Present		7D

Structure	Level	Pattern	Image	Note
TS15: somite	Present		7E

Structure	Level	Pattern	Image	Note
TS15: somite	Present	Regionally restricted	7F	Authors report expression irregularly distributed. Expressing somites reduced in number and stripes weaker and thinner than in Bn/Y embryos. Expression was asymmetric.

Structure	Level	Pattern	Image	Note
TS15: somite	Present	Regionally restricted	7G	Authors report expression irregularly distributed. Expressing somites reduced in number and stripes weaker and thinner than in Bn/Y embryos. Expression was asymmetric.