Antibody:	anti-HNF-4
Detection system:	Secondary antibody coupled to Cy3

Structure	Level	Pattern	Image	Note
TS28: lung epithelium	Present	Regionally restricted	2E	Expression was restricted to ciliated cells as indicated by co-staining with the cilia-specific marker Tubb4.

Structure	Level	Pattern	Image	Note
TS28: nasal epithelium	Present	Regionally restricted	3C	Expression was detected in ciliated cells colocalized with Tubb4.
TS28: olfactory epithelium	Absent		3C

Structure	Level	Pattern	Image	Note
TS28: epithelial layer of oviduct	Present	Regionally restricted	4C	Expression was detected in ciliated cells colocalized with Tubb4.

Structure	Level	Pattern	Image	Note
TS28: brain ependyma	Present	Regionally restricted	4F	Expression was detected in ciliated cells colocalized with Tubb4.

Structure	Level	Pattern	Image	Note
TS23: lung epithelium	Present	Regionally restricted	5D	Expression was detected in approximately half of the proximal airway epithelial cells at E15.5. The ciliated cell marker Tubb4 was first detected at E15.5 and was restricted to a small number of airway epithelial cells that express Foxj1.

Structure	Level	Pattern	Image	Note
TS25: lung epithelium	Present	Regionally restricted		Tracking expression in the airway epithelial cells revealed that the number of cells co-expressing Tubb4 and Foxj1 gradually increased at E17.5.

Structure	Level	Pattern	Image	Note
TS26: lung epithelium	Present	Regionally restricted		Tracking expression in the airway epithelial cells revealed that the number of cells co-expressing Tubb4 and Foxj1 gradually increased at E18.5.

Structure	Level	Pattern	Image	Note
TS27: lung epithelium	Present	Regionally restricted	5F	At birth, expression was detected throughout the proximal airway in a pattern similar to the adult lung. Almost every cell co-expressed Tubb4.

Structure	Level	Pattern	Image	Note
TS28: spermatid	Present		6F	Expression was detected in the nucleus of early spermatids (stage III) and was associated with cytoplasmic expression of Tubb4.

Structure	Level	Pattern	Image	Note
TS28: spermatid	Absent		6G	Expression was absent in spermatozoa and spermatids at stages VII to VIII.
TS28: spermatozoon	Absent		6G	Expression was absent in spermatozoa and spermatids at stages VII to VIII.

Structure	Level	Pattern	Image	Note
TS28: spermatid	Absent		6H	Expression was absent in maturing spermatids.
TS28: spermatocyte	Present		6H	Expression was detected in the cytoplams of spermatocytes at stages XI through XIII.