Antibody:	Anti-Beta-Tubulin IV ONS1A6
Detection system:	Secondary antibody coupled to fluorescein
Assay notes:	The specificity of the antibody used was not detailed; both family members have been annotated.

Structure	Level	Pattern	Image	Note
TS28: lung epithelium	Present	Regionally restricted	2E	Expression was detected in ciliated cells.

Structure	Level	Pattern	Image	Note
TS28: nasal epithelium	Present	Regionally restricted	3C	Expression was detected in ciliated cells colocalized with Foxj1.
TS28: olfactory epithelium	Absent		3C

Structure	Level	Pattern	Image	Note
TS27: nasal epithelium	Present	Regionally restricted		At birth, the epithelial cells of the nose were ciliated.

Structure	Level	Pattern	Image	Note
TS28: epithelial layer of oviduct	Present	Regionally restricted	4C	Expression was detected in ciliated cells.

Structure	Level	Pattern	Image	Note
TS28: brain ependyma	Present	Regionally restricted	4F	Expression was detected in ciliated cells.

Structure	Level	Pattern	Image	Note
TS22: lung epithelium	Absent

Structure	Level	Pattern	Image	Note
TS23: lung epithelium	Present	Regionally restricted	5D	Expression was first detected at E15.5 and was restricted to a small number of airway epithelial cells that express Foxj1.

Structure	Level	Pattern	Image	Note
TS25: lung epithelium	Present	Regionally restricted		Tracking expression in the airway epithelial cells revealed that the number of cells co-expressing Tubb4 and Foxj1 gradually increased at E17.5.

Structure	Level	Pattern	Image	Note
TS26: lung epithelium	Present	Regionally restricted		Tracking expression in the airway epithelial cells revealed that the number of cells co-expressing Tubb4 and Foxj1 gradually increased at E18.5.

Structure	Level	Pattern	Image	Note
TS27: lung epithelium	Present	Regionally restricted	5F	At birth, expression was detected throughout the proximal airway. Almost every cell co-expressed Foxj1.

Structure	Level	Pattern	Image	Note
TS28: spermatid	Present		6F	Expression was detected in the cytoplasm of spermatids and was associated with Foxj1 staining. Expression was detected in the manchette in the innermost layer of more mature spermatids.

Structure	Level	Pattern	Image	Note
TS28: spermatozoon	Present	Regionally restricted	6G	Expression was detected in flagella of mature spermatozoa.

Structure	Level	Pattern	Image	Note
TS28: spermatid	Present	Regionally restricted	6H	Expression was detected in the manchette in the innermost layer of more mature spermatids.