Antibody:	anti-HNF-4
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS28: lung epithelium	Present	Regionally restricted	2A	Expression was detected in a subpopulation of proximal airway epithelial cells.

Structure	Level	Pattern	Image	Note
TS28: bronchus epithelium	Present		2D
TS28: alveolus epithelium	Absent		2D
TS28: respiratory system blood vessel endothelium	Absent		2D

Structure	Level	Pattern	Image	Note
TS28: lung epithelium	Present	Regionally restricted	2F	Double immunostaining revealed that expression of Foxj1 and Scgb1a1 in the airway are in two mutually exclusive cell populations.

Structure	Level	Pattern	Image	Note
TS28: nasal epithelium	Present	Regionally restricted	3A	Expression was detected in ciliated cells in a predominant nuclear pattern.
TS28: paranasal sinus	Present	Regionally restricted	3A	Expression was detected in ciliated cells in a predominant nuclear pattern.

Structure	Level	Pattern	Image	Note
TS28: nasal epithelium	Present	Regionally restricted	3B	Expression was detected in ciliated cells in a predominant nuclear pattern.

Structure	Level	Pattern	Image	Note
TS23: nasal epithelium	Present	Regionally restricted	3E	Expression was detected in nonciliated cells.

Structure	Level	Pattern	Image	Note
TS28: epithelial layer of oviduct	Strong	Regionally restricted	4A , 4B	High expression was detected in proximal oviduct within almost every ciliated epithelial cell lining the lumen. There are fewer ciliated cells in distal oviduct, and in these regions expression is found in fewer cells. Secretory, nonciliated cells do not show expression.

Structure	Level	Pattern	Image	Note
TS23: choroid plexus	Present		4D

Structure	Level	Pattern	Image	Note
TS28: brain ependyma	Present	Regionally restricted	4E	Expression was detected in the ependymal cells lining the ventricles of the brain in a predominantly nuclear pattern.

Structure	Level	Pattern	Image	Note
TS28: bladder	Absent
TS28: heart	Absent
TS28: large intestine	Absent
TS28: liver	Absent
TS28: metanephros	Absent
TS28: skeletal muscle	Absent
TS28: skin	Absent
TS28: small intestine	Absent
TS28: spleen	Absent
TS28: stomach	Absent

Structure	Level	Pattern	Image	Note
TS22: lung epithelium	Absent		5A

Structure	Level	Pattern	Image	Note
TS23: lung epithelium	Present	Regionally restricted	5C	Expression was detected in approximately half of the proximal airway epithelial cells at E15.5.

Structure	Level	Pattern	Image	Note
TS27: lung epithelium	Present	Regionally restricted	5E	At birth, expression was detected throughout the proximal airway in a pattern similar to the adult lung.

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Weak		6A	Weak expression was detected in the cytoplasm of secondary spermatocytes.
TS28: spermatid	Absent		6A	No expression was detected in spermatids (stage I) during early maturation.

Structure	Level	Pattern	Image	Note
TS28: spermatid	Strong		6B	Abundant expression was detected in the nucleus in spermatids at stages III to IV.

Structure	Level	Pattern	Image	Note
TS28: spermatid	Strong		6C	Abundant expression was detected in the nucleus in spermatids at stages V to VI.

Structure	Level	Pattern	Image	Note
TS28: spermatid	Absent		6D	After stage VII, expression was extinguished in spermatids while development of the flagella continued.
TS28: spermatocyte	Present		6D	Expression was detected in the cytoplasm of spermatocytes at stages VII to VIII.

Structure	Level	Pattern	Image	Note
TS28: spermatid	Absent		6E	After stage VII, expression was extinguished in spermatids while development of the flagella continued.
TS28: spermatocyte	Weak		6E	Weak expression was detected in spermatocytes at stages XI through XIII.