Summary | Mutation origin | Mutation description | Find Mice (IMSR) | Notes | References

Symbol:	Col1a1tm1(tetO-GFP/RNAi:rluc)Begg
Name:	collagen, type I, alpha 1; targeted mutation 1, Alan Beggs
MGI ID:	MGI:6368177
Synonyms:	shRNA Ren
Gene:	Col1a1  Location: Chr11:94827050-94843868 bp, + strand  Genetic Position: Chr11, 59.01 cM, cytoband D
Alliance:	Col1a1tm1(tetO-GFP/RNAi:rluc)Begg page

Germline Transmission:	Earliest citation of germline transmission: J:341566
Parent Cell Line:	KH2 (ES Cell)
Strain of Origin:	(C57BL/6 x 129S4/SvJae)F1

Allele Type:    Targeted (Inducible, Knockdown, Reporter) Inducer:    doxycycline/tetracycline Mutation:    Insertion   Mutation details: RMCE (recombinase-mediated cassette exchange) was utilized to insert tetO-GFP/RNAi:Renilla luciferase (rluc) into the Col1a1 locus. The vector contains a tetracycline responsive promoter (TRE;tetO) controlling expression of a GFP reporter and short-hairpin RNA sequence targeting luciferase embedded in the MIR30 structure. The vector was then co-electroporated with a FLPe recombinase-expressing vector into the RMCE-ready KH2 ES cells. (J:341566)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Col1a1 Mutation:	160 strains or lines available

Original:	J:341566 Kazerounian S, et al., Rpl5-Inducible Mouse Model for Studying Diamond-Blackfan Anemia. Discoveries (Craiova). 2019 Sep 30;7(3):e96
All:	1 reference(s)