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Gene Expression Data
Assay Details
Assay
Reference: J:226694 Matsuzaki H, et al., De novo DNA methylation through the 5'-segment of the H19 ICR maintains its imprint during early embryogenesis. Development. 2015 Nov 15;142(22):3833-44
Assay type: RT-PCR
MGI Accession ID: MGI:6368115
Gene symbol: Dnmt3a
Gene name: DNA methyltransferase 3A
Probe: 3a-ex17F, 3a-ex18R
Probe preparation: labelled with SYBR green
Assay notes: Quantitative RT-PCR. Relative expression is shown normalized to H2afz expression.
Results
Image: S2B ©

 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified Amount Genetic BackgroundMutant Allele(s)Sex Note
MII oocyte P adult TS28: secondary oocyte Present Not Specified; total RNA involves: 129S4/SvJae * C57BL/6J Tg(Zp3-cre)93Knw/0Female
1-cell E0.5 TS1: 1-cell stage conceptus Present Not Specified; total RNA involves: 129S4/SvJae * C57BL/6J Tg(Zp3-cre)93Knw/0Not Specified
2-cell E1.0 TS2: 2-cell stage conceptus Present Not Specified; total RNA involves: 129S4/SvJae * C57BL/6J Tg(Zp3-cre)93Knw/0Not Specified
Blastocyst E3.5 TS4: blastocyst-stage conceptus Present Not Specified; total RNA involves: 129S4/SvJae * C57BL/6J Tg(Zp3-cre)93Knw/0Not Specified
MII oocyte P adult TS28: secondary oocyte Absent Not Specified; total RNA involves: 129S4/SvJae * C57BL/6J Dnmt3atm3.1Enl/Dnmt3atm3.1Enl, Tg(Zp3-cre)93Knw/0Female Conditional mutant.
1-cell E0.5 TS1: 1-cell stage conceptus Absent Not Specified; total RNA involves: 129S4/SvJae * C57BL/6J Dnmt3atm3.1Enl/Dnmt3atm3.1Enl, Tg(Zp3-cre)93Knw/0Not Specified Conditional mutant.
2-cell E1.0 TS2: 2-cell stage conceptus Absent Not Specified; total RNA involves: 129S4/SvJae * C57BL/6J Dnmt3atm3.1Enl/Dnmt3atm3.1Enl, Tg(Zp3-cre)93Knw/0Not Specified Conditional mutant.
Blastocyst E3.5 TS4: blastocyst-stage conceptus Present Not Specified; total RNA involves: 129S4/SvJae * C57BL/6J Dnmt3atm3.1Enl/Dnmt3atm3.1Enl, Tg(Zp3-cre)93Knw/0Not Specified Conditional mutant.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory