Gene Expression Data
Assay Details
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Assay
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Reference:
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J:262633
Shukunami C, et al., Scleraxis is a transcriptional activator that regulates the expression of Tenomodulin, a marker of mature tenocytes and ligamentocytes. Sci Rep. 2018 Feb 16;8(1):3155
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:6362639
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Gene symbol:
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Tnmd
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Gene name:
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tenomodulin
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Probe:
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TeM cDNA1
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
1a:
embryonic day 12.5
(more  )
Specimen
1a:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: back connective tissue |
Present |
Regionally restricted |
1a
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Expression was detected in the developing axial tendons along the cervical and thoracic spine.
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| TS20: limb |
Weak |
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1a
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Specimen
1b:
embryonic day 12.5
(more )
Specimen
1b:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: forelimb tendon primordium |
Present |
Regionally restricted |
1b
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Expression was detected in the primordia of the triceps brachii tendon and extensor digitorum communis tendon.
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Specimen
1d:
embryonic day 13.5
(more )
Specimen
1d:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: back connective tissue |
Present |
Regionally restricted |
1d
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Expression was detected in the developing axial tendons along the cervical and thoracic spine.
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Specimen
1e:
embryonic day 13.5
(more )
Specimen
1e:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: forelimb tendon primordium |
Present |
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1e
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Expression was detected in developing forelimb tendons including the developing triceps branchii tendon, the extensor digitorum communis tendon, the developing extensor carpi radialis brevis/longus tendon, and the extensor carpi radialis tendon.
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Specimen
3e:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Scxem13Shuk/Scx+
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Age:
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postnatal day 1
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
3h:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Scxem13Shuk/Scxem13Shuk
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Age:
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postnatal day 1
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
3k:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Scxem13Shuk/Scx+
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Age:
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postnatal day 1
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
3n:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Scxem13Shuk/Scxem13Shuk
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Age:
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postnatal day 1
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: tendo calcaneus |
Weak |
Regionally restricted |
3n
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Expression was faint in the proximal portion and undetectable in the distal region of the Achilles tendon in the mutant.
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Analysis Tools