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Gene Expression Data
Assay Details
Assay
Reference: J:106405 Mackay DR, et al., The mouse Ovol2 gene is required for cranial neural tube development. Dev Biol. 2006 Mar 1;291(1):38-52
Assay type: RNA in situ
MGI Accession ID: MGI:6360623
Gene symbol: Shh
Gene name: sonic hedgehog
Probe: Shh cDNA3
Probe preparation: Antisense labelled with digoxigenin RNA
Visualized with: Alkaline phosphatase
Results
Specimen 5D +/-: embryonic day 8.5; Ovol2tm1Xdai/Ovol2+ (more )
Structure Level Pattern Image Note
TS13: notochord Present 5D +/-
TS13: axial mesoderm Present Regionally restricted 5D +/- Expression in axial mesoderm of prechordal plate.
Specimen 5D -/-: embryonic day 8.5; Ovol2tm1Xdai/Ovol2tm1Xdai (more )
Structure Level Pattern Image Note
TS13: notochord Present 5D -/- Authors state expression in notochord appears normal in mutant.
TS13: axial mesoderm Present Regionally restricted 5D -/- Expression in prechordal plate region was not as rostrally extended as in wildtype, and lacked characteristic hook-shaped morphology.
Specimen 5E +/-: embryonic day 8.75; Ovol2tm1Xdai/Ovol2+ (more )
Structure Level Pattern Image Note
TS14: axial mesoderm Present Regionally restricted 5E +/- Expression in axial mesoderm of prechordal plate.
TS14: future brain Present Regionally restricted 5E +/- Expression in ventral aspect of brain.
Specimen 5E -/-: embryonic day 8.75; Ovol2tm1Xdai/Ovol2tm1Xdai (more )
Structure Level Pattern Image Note
TS14: axial mesoderm Present Regionally restricted 5E -/- Expression in axial mesoderm of prechordal plate.
TS14: future brain Weak Regionally restricted 5E -/- Weaker expression in ventral forebrain.
Specimen 5F +/-: embryonic day 8.75; Ovol2tm1Xdai/Ovol2+ (more )
Structure Level Pattern Image Note
TS14: axial mesoderm Present Regionally restricted 5F +/- Expression in prechordal plate.
Specimen 5F -/-: embryonic day 8.75; Ovol2tm1Xdai/Ovol2tm1Xdai (more )
Structure Level Pattern Image Note
TS14: axial mesoderm Present Regionally restricted 5F -/- Expression was more diffuse in truncated prechordal plate.
Specimen 5G +/-: embryonic day 9.5; Ovol2tm1Xdai/Ovol2+ (more )
Structure Level Pattern Image Note
TS15: axial mesoderm Present Regionally restricted 5G +/- Expression in prechordal plate.
TS15: future brain Present Regionally restricted 5G +/- Expression in ventral aspect of brain.
Specimen 5G -/-: embryonic day 9.5; Ovol2tm1Xdai/Ovol2tm1Xdai (more )
Structure Level Pattern Image Note
TS15: axial mesoderm Weak Regionally restricted 5G -/- Reduced expression in mutant prechordal plate.
TS15: future brain Present Regionally restricted 5G -/- Gap within expressing domain in ventral forebrain.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory