Gene Expression Data
Assay Details
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Assay
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Reference:
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J:104974
MacDonald JL, et al., Stage-specific induction of DNA methyltransferases in olfactory receptor neuron development. Dev Biol. 2005 Dec 15;288(2):461-73
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6360288
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Gene symbol:
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Hdac2
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Gene name:
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histone deacetylase 2
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Antibody:
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anti-Hdac2
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Detection system:
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Secondary antibody coupled to Alexa Fluor 488
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Results
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Specimen
6A:
embryonic day 17.0
(more  )
Note: Double labeled: green - Hdac2; red - Pcna (assay MGI:6360276).
Specimen
6A:
(close  )
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Genetic Background:
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CD-1
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Age:
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embryonic day 17.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Hdac2; red - Pcna (assay MGI:6360276).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: olfactory epithelium |
Present |
Regionally restricted |
6A
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Co-expression in small subpopulation of Pcna-expressing cells in both basal and middle layers.
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Specimen
6B:
postnatal day 5
(more )
Note: Double labeled: green - Hdac2; red - Pcna (assay MGI:6360276).
Specimen
6B:
(close )
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Genetic Background:
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CD-1
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Age:
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postnatal day 5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Hdac2; red - Pcna (assay MGI:6360276).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: olfactory epithelium |
Present |
Regionally restricted |
6B
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Co-expression in small subpopulation of Pcna-expressing cells in both basal and middle layers.
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Specimen
6C:
postnatal adult
(more )
Note: Double labeled: green - Hdac2; red - Omp (assay MGI:6360279).
Specimen
6C:
(close )
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Genetic Background:
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CD-1
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Hdac2; red - Omp (assay MGI:6360279).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: olfactory epithelium |
Present |
Regionally restricted |
6C
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Expression only in a small population of Omp-expressing cells.
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Specimen
6D:
embryonic day 17.0
(more )
Note: Double labeled: green - Hdac2; red - Dnmt3b (assay MGI:6360281).
Specimen
6D:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 17.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Hdac2; red - Dnmt3b (assay MGI:6360281).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: olfactory epithelium |
Present |
Regionally restricted |
6D
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Co-expression in subpopulation of basally situated Dnmt3b-expressing cells.
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Specimen
6E:
postnatal day 5
(more )
Note: Double labeled: green - Hdac2; red - Dnmt3b (assay MGI:6360281).
Specimen
6E:
(close )
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Genetic Background:
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CD-1
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Age:
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postnatal day 5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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|
Note:
|
Double labeled: green - Hdac2; red - Dnmt3b (assay MGI:6360281).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: olfactory epithelium |
Present |
Regionally restricted |
6E
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Co-expression in subpopulation of basally situated Dnmt3b-expressing cells.
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Specimen
6F:
postnatal adult
(more )
Note: Double labeled: green - Hdac2; red - Dnmt3b (assay MGI:6360281).
Specimen
6F:
(close )
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Genetic Background:
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CD-1
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
|
|
Note:
|
Double labeled: green - Hdac2; red - Dnmt3b (assay MGI:6360281).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: olfactory epithelium |
Present |
Regionally restricted |
6F
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Co-expression in subpopulation of basally situated Dnmt3b-expressing cells.
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Specimen
6G:
embryonic day 17.0
(more )
Note: Double labeled: green - Hdac2; red - Dnmt3a (assay MGI:6360286).
Specimen
6G:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 17.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
|
Double labeled: green - Hdac2; red - Dnmt3a (assay MGI:6360286).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: olfactory epithelium |
Present |
Regionally restricted |
6G
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Co-expression in many Dnmt3a-expressing cells. Most basal cells express Hdac2 exclusively.
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Specimen
6H:
postnatal day 5
(more )
Note: Double labeled: green - Hdac2; red - Dnmt3a (assay MGI:6360286).
Specimen
6H:
(close )
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Genetic Background:
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CD-1
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Age:
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postnatal day 5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
|
|
Note:
|
Double labeled: green - Hdac2; red - Dnmt3a (assay MGI:6360286).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: olfactory epithelium |
Present |
Regionally restricted |
6H
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Co-expression in many Dnmt3a-expressing cells. Most basal cells express Hdac2 exclusively.
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Specimen
6I:
postnatal adult
(more )
Note: Double labeled: green - Hdac2; red - Dnmt3a (assay MGI:6360286).
Specimen
6I:
(close )
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Genetic Background:
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CD-1
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
|
|
Note:
|
Double labeled: green - Hdac2; red - Dnmt3a (assay MGI:6360286).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: olfactory epithelium |
Present |
Regionally restricted |
6I
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Co-expression in many Dnmt3a-expressing cells.
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Specimen
7E:
postnatal adult
(more )
Note: Double labeled: green - Hdac2; red - Pcna (assay MGI:6360276).
Specimen
7E:
(close )
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Genetic Background:
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CD-1
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: green - Hdac2; red - Pcna (assay MGI:6360276).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: olfactory epithelium |
Present |
Regionally restricted |
7E
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Expression in only a subset of Pcna-expressing cells.
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Specimen
7F:
postnatal adult
(more )
Note: Double labeled: green - Hdac2; red - Tubb3 (assay MGI:6360285).
Specimen
7F:
(close )
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Genetic Background:
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CD-1
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
|
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Note:
|
Double labeled: green - Hdac2; red - Tubb3 (assay MGI:6360285).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: olfactory epithelium |
Present |
Regionally restricted |
7F
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Expression mostly coinciding with Tubb3 expression.
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Specimen
7G:
postnatal adult
(more )
Note: Double labeled: green - Hdac2; red - Omp (assay MGI:6360279).
Specimen
7G:
(close )
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Genetic Background:
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CD-1
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: green - Hdac2; red - Omp (assay MGI:6360279).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: olfactory epithelium |
Present |
Regionally restricted |
7G
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Expression in a subset of Omp-expressing cells.
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Specimen
7H:
postnatal adult
(more )
Note: Double labeled: green - Hdac2; red - Dnmt3b (assay MGI:6360281).
Specimen
7H:
(close )
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Genetic Background:
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CD-1
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Age:
|
postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: green - Hdac2; red - Dnmt3b (assay MGI:6360281).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: olfactory epithelium |
Present |
Regionally restricted |
7H
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Expression in a subset of Dnmt3b-expressing cells.
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Analysis Tools