Cux1 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:258043 Wang Y, et al., N(6)-methyladenosine RNA modification regulates embryonic neural stem cell self-renewal through histone modifications. Nat Neurosci. 2018 Feb;21(2):195-206
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:6358399
Gene symbol:	Cux1
Gene name:	cut-like homeobox 1

Antibody:	anti-Cux1 (CDP, M-222) (sc-13024)
Detection system:	Secondary antibody coupled to Alexa Fluor 488

Results

Specimen 4a Mettl14f/f: postnatal day 0; Mettl14^tm1.1Czhao/Mettl14^tm1.1Czhao (more

)

Structure	Level	Image	Note
TS27: cortical layer II	Present	4a Cux1 Mettl14f/f , 4a Cux1 merge Mettl14f/f	Expression is in late-born neurons.
TS27: cortical layer III	Present	4a Cux1 Mettl14f/f , 4a Cux1 merge Mettl14f/f	Expression is in late-born neurons.
TS27: cortical layer IV	Present	4a Cux1 Mettl14f/f , 4a Cux1 merge Mettl14f/f	Expression is in late-born neurons.

Specimen 4a Mettl14f/f;Nestin-cre: postnatal day 0; Mettl14^tm1.1Czhao/Mettl14^tm1.1Czhao, Tg(Nes-cre)1Kln/0 (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS27: cortical layer II	Present	Regionally restricted	4a Cux1 merge Mettl14f/f;Nestin-cre , 4a Cux1 Mettl14f/f;Nestin-cre	This mutant has reduced thickness (70%) of the Cux1-positive layers (II-IV).
TS27: cortical layer III	Present	Regionally restricted	4a Cux1 merge Mettl14f/f;Nestin-cre , 4a Cux1 Mettl14f/f;Nestin-cre	This mutant has reduced thickness (70%) of the Cux1-positive layers (II-IV).
TS27: cortical layer IV	Present	Regionally restricted	4a Cux1 merge Mettl14f/f;Nestin-cre , 4a Cux1 Mettl14f/f;Nestin-cre	This mutant has reduced thickness (70%) of the Cux1-positive layers (II-IV).

Specimen 4a Mettl14f/+;Nestin-cre: postnatal day 0; Mettl14^tm1.1Czhao/Mettl14⁺, Tg(Nes-cre)1Kln/0 (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS27: cortical layer II	Present	Regionally restricted	4a Cux1 Mettl14f/+;Nestin-cre , 4a Cux1 merge Mettl14f/+;Nestin-cre	Expression is in late-born neurons.
TS27: cortical layer III	Present	Regionally restricted	4a Cux1 Mettl14f/+;Nestin-cre , 4a Cux1 merge Mettl14f/+;Nestin-cre	Expression is in late-born neurons.
TS27: cortical layer IV	Present	Regionally restricted	4a Cux1 Mettl14f/+;Nestin-cre , 4a Cux1 merge Mettl14f/+;Nestin-cre	Expression is in late-born neurons.

Specimen 4e Mettl14f/f: embryonic day 17.5; Mettl14^tm1.1Czhao/Mettl14^tm1.1Czhao (more

)

Structure	Level	Pattern	Image	Note
TS25: cerebral cortex ventricular zone	Present		4e Mettl14f/f , 4e merge Mettl14f/f

Specimen 4e Mettl14f/f;Nestin-cre: embryonic day 17.5; Mettl14^tm1.1Czhao/Mettl14^tm1.1Czhao, Tg(Nes-cre)1Kln/0 (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS25: cerebral cortex ventricular zone	Present	Regionally restricted	4e Mettl14f/f;Nestin-cre , 4e merge Mettl14f/f;Nestin-cre	There is a a 22% reduction in the thickness of Cux1-expressing layers and a 50% reduction in the number of newly generated expressing cells in this mutant versus controls.

Specimen 4e Mettl14f/f;Nestin-cre: embryonic day 17.5; Mettl14^tm1.1Czhao/Mettl14⁺, Tg(Nes-cre)1Kln/0 (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS25: cerebral cortex ventricular zone	Present		4e Mettl14f/+;Nestin-cre , 4e merge Mettl14f/+;Nestin-cre

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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