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Gene Expression Data
Assay Details
Assay
Reference: J:170258 Mavromatakis YE, et al., Foxa1 and Foxa2 positively and negatively regulate Shh signalling to specify ventral midbrain progenitor identity. Mech Dev. 2011 Jan-Feb;128(1-2):90-103
Assay type: RNA in situ
MGI Accession ID: MGI:6355445
Gene symbol: Gli2
Gene name: GLI-Kruppel family member GLI2
Probe: pGli-2
Results
Specimen 6I: embryonic day 9.5; Foxa2tm1Khk/Foxa2tm1Khk (more )
Note: Control.
Structure Level Pattern Image Note
TS15: future midbrain Present Regionally restricted 6I The ventral limit of expression was restricted to the alar/basal boundary.
Specimen 6I': embryonic day 9.5; Foxa2tm1Khk/Foxa2tm1Khk, H2az2Tg(Wnt1-cre)11Rth/H2az2+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS15: future midbrain Present Regionally restricted 6I' In the conditional knockout the expression was shifted ventrally so that basal progenitors maintained expression.
Specimen 6J: embryonic day 10.5; Foxa2tm1Khk/Foxa2tm1Khk (more )
Note: Control. Posterior Midbrain.
Structure Level Pattern Image Note
TS17: midbrain Present Regionally restricted 6J The ventral limit of expression was restricted to the alar/basal boundary.
Specimen 6J': embryonic day 10.5; Foxa2tm1Khk/Foxa2tm1Khk, H2az2Tg(Wnt1-cre)11Rth/H2az2+ (more )
Note: Conditional mutant. Posterior Midbrain.
Structure Level Pattern Image Note
TS17: midbrain Present Regionally restricted 6J' In the posterior midbrain, conditional knockout expression was shifted ventrally so that basal progenitors maintained expression.
Specimen 6K: embryonic day 10.5; Foxa2tm1Khk/Foxa2tm1Khk (more )
Note: Control. Anterior Midbrain.
Structure Level Pattern Image Note
TS17: midbrain Present Regionally restricted 6K The ventral limit of expression was restricted to the alar/basal boundary.
Specimen 6K': embryonic day 10.5; Foxa2tm1Khk/Foxa2tm1Khk, H2az2Tg(Wnt1-cre)11Rth/H2az2+ (more )
Note: Conditional mutant. Anterior Midbrain.
Structure Level Pattern Image Note
TS17: midbrain Present Regionally restricted 6K' The normal pattern of expression was recovered in the anterior midbrain of the E10.5 conditional knockout.
Specimen 6L: embryonic day 12.5; Foxa1tm1Gsc/Foxa1tm1Gsc, Foxa2tm1Khk/Foxa2+ (more )
Note: Control; genotype either Foxa fl/fl; Foxa1 +/+ or Foxa2 fl/fl; Foxa1 +/-
Structure Level Pattern Image Note
TS20: midbrain Present Regionally restricted 6L The ventral limit of expression was restricted to the alar/basal boundary.
Specimen 6L': embryonic day 12.5; Foxa2tm1Khk/Foxa2tm1Khk, H2az2Tg(Wnt1-cre)11Rth/H2az2+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS20: midbrain Present Regionally restricted 6L' The normal pattern of expression was detected in the midbrain of the E12.5 conditional knockout.
Specimen 6L'': embryonic day 12.5; Foxa1tm1Gsc/Foxa1tm1Gsc, Foxa2tm1Khk/Foxa2tm1Khk (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS20: midbrain Present Regionally restricted 6L'' In the double mutant conditional knockout, expression was restricted to the ventral midline of the midbrain.
Specimen S1 E9.25: embryonic day 9.25 (more )
Structure Level Pattern Image Note
TS15: future midbrain Present Regionally restricted S1_Gli2 E9.25 Compared to an adjacent section, Gli2 expression overlaps with Foxa2 at E9.25.
Specimen S1 E9.5: embryonic day 9.5 (more )
Structure Level Pattern Image Note
TS15: future midbrain Present Regionally restricted S1_Gli2 E9.5 Expression becomes progressively restricted to the alar/basal boundary.
Specimen S1 E12.5: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: midbrain Present Regionally restricted S1_Gli2 E12.5 Compared to adjacent sections, the ventral limit of Gli2 expression abuts the dorsal limit of Foxa2 expression at the alar/basal boundary, and there is a gap between Shh and Gli2 expression in the ventral midbrain.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory