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Gene Expression Data
Assay Details
Assay
Reference: J:170258 Mavromatakis YE, et al., Foxa1 and Foxa2 positively and negatively regulate Shh signalling to specify ventral midbrain progenitor identity. Mech Dev. 2011 Jan-Feb;128(1-2):90-103
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6343553
Gene symbol: Foxa2
Gene name: forkhead box A2
Antibody: anti-HNF3b
Assay notes: Two antibodies were described, but the one used in this assay was not specified. Either MGI:1338477 or MGI:5562562 could have been used.
Results
Specimen 1B E10.5 Control: embryonic day 10.5; Foxa2tm1Khk/Foxa2tm1Khk (more )
Structure Level Pattern Image Note
TS17: midbrain Present Regionally restricted 1B E10.5 Control Expression was detected in the ventral midbrain.
Specimen 1B E10.5 Foxa2-Wnt1 cko: embryonic day 10.5; Foxa2tm1Khk/Foxa2tm1Khk, H2az2Tg(Wnt1-cre)11Rth/H2az2+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS17: midbrain floor plate Present Regionally restricted 1B E10.5 Foxa2-Wnt1 cko Expression in the conditional mutant is deleted from the ventral midbrain except in the medial floor plate.
Specimen 1B E12.5 Control: embryonic day 12.5; Foxa2tm1Khk/Foxa2tm1Khk (more )
Structure Level Pattern Image Note
TS20: midbrain Present Regionally restricted 1B E12.5 Control Expression was detected in the ventral midbrain.
Specimen 1B E12.5 Foxa2-Wnt1 cko: embryonic day 12.5; Foxa2tm1Khk/Foxa2tm1Khk, H2az2Tg(Wnt1-cre)11Rth/H2az2+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS20: midbrain floor plate Present Regionally restricted 1B E12.5 Foxa2-Wnt1 cko Expression in the conditional mutant is deleted from the ventral midbrain except in the medial floor plate.
Specimen 5A: embryonic day 9.5; Foxa2tm1Khk/Foxa2tm1Khk (more )
Note: Control. Double labeled: red - Foxa2; green - Nkx2-2 (assay MGI:6343569).
Structure Level Pattern Image Note
TS15: future midbrain Present Regionally restricted 5A Expression was detected in the ventral midbrain and overlaps with Nkx2-2.
Specimen 5B: embryonic day 9.5; Foxa2tm1Khk/Foxa2tm1Khk, H2az2Tg(Wnt1-cre)11Rth/H2az2+ (more )
Note: Conditional mutant. Double labeled: red - Foxa2; green - Nkx2-2 (assay MGI:6343569).
Structure Level Pattern Image Note
TS15: future midbrain floor plate Present Regionally restricted 5B
Specimen 5C: embryonic day 10.5; Foxa2tm1Khk/Foxa2tm1Khk (more )
Note: Control. Double labeled: red - Foxa2; green - Nkx2-2 (assay MGI:6343569).
Structure Level Pattern Image Note
TS17: midbrain Present Regionally restricted 5C Expression was detected in the ventral midbrain. Co-expression with Nkx2-2 was progressively becoming restricted to the alar/basal plate boundary.
Specimen 5D: embryonic day 10.5; Foxa2tm1Khk/Foxa2tm1Khk, H2az2Tg(Wnt1-cre)11Rth/H2az2+ (more )
Note: Conditional mutant. Double labeled: red - Foxa2; green - Nkx2-2 (assay MGI:6343569).
Structure Level Pattern Image Note
TS17: midbrain floor plate Present Regionally restricted 5D
Specimen 5E: embryonic day 11.5; Foxa2tm1Khk/Foxa2tm1Khk (more )
Note: Control. Double labeled: red - Foxa2; green - Nkx2-2 (assay MGI:6343569).
Structure Level Pattern Image Note
TS19: midbrain Present Regionally restricted 5E Expression was detected in the ventral midbrain. Co-expression with Nkx2-2 was progressively becoming restricted to the alar/basal plate boundary.
Specimen 5F: embryonic day 11.5; Foxa2tm1Khk/Foxa2tm1Khk, H2az2Tg(Wnt1-cre)11Rth/H2az2+ (more )
Note: Conditional mutant. Double labeled: red - Foxa2; green - Nkx2-2 (assay MGI:6343569).
Structure Level Pattern Image Note
TS19: midbrain floor plate Present Regionally restricted 5F
Specimen 5G: embryonic day 12.5; Foxa2tm1Khk/Foxa2tm1Khk (more )
Note: Control. Double labeled: red - Foxa2; green - Nkx2-2 (assay MGI:6343569).
Structure Level Pattern Image Note
TS20: midbrain Present Regionally restricted 5G Expression was detected in the ventral midbrain. Co-expression with Nkx2-2 was progressively becoming restricted to the alar/basal plate boundary.
Specimen 5H: embryonic day 12.5; Foxa2tm1Khk/Foxa2tm1Khk, H2az2Tg(Wnt1-cre)11Rth/H2az2+ (more )
Note: Conditional mutant. Double labeled: red - Foxa2; green - Nkx2-2 (assay MGI:6343569).
Structure Level Pattern Image Note
TS20: midbrain floor plate Present Regionally restricted 5H
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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