Antibody:	anti-MMP-13
Detection system:	fluorescein
Assay notes:	Fixation with acid alcohol.

Structure	Level	Pattern	Image	Note
TS20: palatal shelf mesenchyme	Strong	Regionally restricted	2A	Relative expression increased within nasal and maxillary regions of mesenchyme. Some expression within medial and central regions. No expression in mid-oral, lateral and tooth germ mesenchyme.
TS20: palatal shelf epithelium	Trace	Regionally restricted	2A	Slight expression in palatal epithelial basement membrane.

Structure	Level	Pattern	Image	Note
TS21: palatal shelf mesenchyme	Strong	Regionally restricted	2B	Expression enhanced in nasal, central and dorsal medial mesenchymal regions. Expression absent from ventral medial, mid-oral and lateral mesenchyme.
TS21: palatal shelf epithelium	Strong	Regionally restricted	2B	Intense expression in palatal basement membrane, particularly in regions subjacent to nasal palatal epithelium and lateral portion of oral palatal epithelium.

Structure	Level	Pattern	Image	Note
TS22: palatal shelf mesenchyme	Strong	Regionally restricted	2C	Expression persists in dorsal medial mesenchyme extending into dorsal regions of central and maxillary mesenchyme. Relative expression decreased within ventral medial, mid-oral or lateral mesenchyme. Within nasal mesenchymal region, expression associated with forming bone. Expression surrounding regressing palatal seam.
TS22: palatal shelf epithelium	Strong	Regionally restricted	2C	Strong expression in all regions of palatal epithelial basememnt membrane, especially notable expression within regressing palatal seam.

Structure	Level	Pattern	Image	Note
TS22: palatal shelf epithelium	Weak	Regionally restricted		Reduced expression, detected only within a band of mesenchyme including ventral medial and portions of mid-oral, central and lateral mesenchyme.