Antibody:

anti-pSmad1/5/8

Structure	Level	Pattern	Image	Note
TS13: neural fold	Strong	Regionally restricted	4C	Predominant expression in dorsal rim of neural folds.
TS13: lateral plate mesoderm	Strong		4C	Predominant expression in lateral plate mesoderm.
TS13: intermediate mesenchyme	Present	Scattered	4C	Expression in scattered cells in intermediate mesoderm.
TS13: paraxial mesenchyme	Present	Regionally restricted		Expression in scattered cells in lateral paraxial mesoderm.

Structure	Level	Pattern	Image	Note
TS13: neural fold	Strong	Regionally restricted	4D	Predominant expression in dorsal rim of neural folds.
TS13: lateral plate mesoderm	Strong		4D	Predominant expression in lateral plate mesoderm.
TS13: intermediate mesenchyme	Present	Scattered	4D	Expression in scattered cells in intermediate mesoderm.
TS13: paraxial mesenchyme	Present	Regionally restricted	4D	Expression in scattered cells in lateral paraxial mesoderm. No significant medial expansion.

Structure	Level	Pattern	Image	Note
TS13: neural fold	Strong	Regionally restricted	4F	Predominant expression in dorsal rim of neural folds.
TS13: lateral plate mesoderm	Strong		4F	Predominant expression in lateral plate mesoderm.
TS13: intermediate mesenchyme	Present	Scattered	4F	Expression in scattered cells in intermediate mesoderm.
TS13: paraxial mesenchyme	Present	Regionally restricted	4F	Expression in scattered cells in lateral paraxial mesoderm. No significant medial expansion.

Structure	Level	Pattern	Image	Note
TS13: intermediate mesenchyme	Present	Scattered	4G	Expression in scattered cells in intermediate mesoderm.
TS13: paraxial mesenchyme	Present	Regionally restricted	4G	Expression in scattered cells in lateral paraxial mesoderm. No significant medial expansion.

Structure	Level	Pattern	Image	Note
TS13: neural fold	Strong	Regionally restricted	4H	Predominant expression in dorsal rim of neural folds.
TS13: lateral plate mesoderm	Strong		4H	Predominant expression in lateral plate mesoderm.
TS13: intermediate mesenchyme	Present	Scattered	4H	Expression in scattered cells in intermediate mesoderm.
TS13: paraxial mesenchyme	Present	Regionally restricted	4H	Expression in scattered cells in lateral paraxial mesoderm. No significant medial expansion.

Structure	Level	Pattern	Image	Note
TS13: neural fold	Strong	Regionally restricted	4I	Predominant expression in dorsal rim of neural folds.
TS13: lateral plate mesoderm	Strong		4I	Predominant expression in lateral plate mesoderm.
TS13: intermediate mesenchyme	Present	Scattered	4I	Expression in scattered cells in intermediate mesoderm.
TS13: paraxial mesenchyme	Present	Regionally restricted	4I	Expression in scattered cells in lateral paraxial mesoderm. No significant medial expansion.

Structure	Level	Pattern	Image	Note
TS13: neural fold	Strong	Regionally restricted	4J	Predominant expression in dorsal rim of neural folds.
TS13: lateral plate mesoderm	Strong		4J	Predominant expression in lateral plate mesoderm.
TS13: intermediate mesenchyme	Present	Scattered	4J	Expression in scattered cells in intermediate mesoderm.
TS13: paraxial mesenchyme	Present	Regionally restricted	4J	Expression in scattered cells in lateral paraxial mesoderm. No significant medial expansion.

Structure	Level	Pattern	Image	Note
TS13: neural fold	Strong	Regionally restricted	4K	Predominant expression in dorsal rim of neural folds.
TS13: lateral plate mesoderm	Strong		4K	Predominant expression in lateral plate mesoderm.
TS13: intermediate mesenchyme	Present	Scattered	4K	Expression in scattered cells in intermediate mesoderm.
TS13: paraxial mesenchyme	Present	Regionally restricted	4K	Expression in scattered cells in lateral paraxial mesoderm. No significant medial expansion.