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Gene Expression Data
Assay Details
Assay
Reference: J:74063 Joronen K, et al., Temporospatial expression of tissue inhibitors of matrix metalloproteinases-1, -2 and -3 during development, growth and aging of the mouse skeleton. Histochem Cell Biol. 2000 Aug;114(2):157-65
Assay type: Northern blot
MGI Accession ID: MGI:6323277
Gene symbol: Timp2
Gene name: tissue inhibitor of metalloproteinase 2
Probe: pMTIMP-2-1
Probe preparation: Double stranded labelled with P32
Visualized with: Autoradiography
Assay notes: Data was summarised in Table 2. Small amount of a 1.7kb transcript were seen in some tissues (not specified).
Results Image: 1B
 Sample Information Bands Other Sample Information
Lane AgeStructure 3.8 kb (a) 1.2 kb (a) Amount Genetic BackgroundMutant Allele(s)Sex
brain P5 TS28: brain Moderate Moderate 10 µg; total RNA Not Specified Not Specified
heart P5 TS28: heart Moderate Weak 10 µg; total RNA Not Specified Not Specified
liver P5 TS28: liver Ambiguous Ambiguous 10 µg; total RNA Not Specified Not Specified
kidney P5 TS28: metanephros Weak Weak 10 µg; total RNA Not Specified Not Specified
lung P5 TS28: lung Present Present 10 µg; total RNA Not Specified Not Specified
stomach P5 TS28: stomach Weak Strong 10 µg; total RNA Not Specified Not Specified
intestine P5 TS28: intestine Moderate Weak 10 µg; total RNA Not Specified Not Specified
thyreoidea P5 TS28: thyroid gland Ambiguous Ambiguous 10 µg; total RNA Not Specified Not Specified
spleen P5 TS28: spleen Weak Weak 10 µg; total RNA Not Specified Not Specified
ovary P5 TS28: ovary Ambiguous Present 10 µg; total RNA Not Specified Female
eye P5 TS28: eye Weak Strong 10 µg; total RNA Not Specified Not Specified
skin P5 TS28: skin Moderate Strong 10 µg; total RNA Not Specified Not Specified
muscle P5 TS28: muscle tissue Moderate Weak 10 µg; total RNA Not Specified Not Specified
calvarial bone P5 TS28: chondrocranium Present Moderate 10 µg; total RNA Not Specified Not Specified
costal cartilage P5 TS28: costal cartilage Weak Weak 10 µg; total RNA Not Specified Not Specified
epiphysis P5 TS28: long bone epiphysis Present Weak 10 µg; total RNA Not Specified Not Specified
metaphysis P5 TS28: long bone metaphysis Weak Weak 10 µg; total RNA Not Specified Not Specified
diaphysis P5 TS28: long bone diaphysis Weak Weak 10 µg; total RNA Not Specified Not Specified
Notes:
(a) Ambiguous, <10%; weak, 10-24%; moderate, 25-49%; present, 50-74%; strong, 75-100% of maximal levels.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/21/2024
MGI 6.23 		The Jackson Laboratory