Antibody:	clone PG-21
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS26: mesenchyme of penile body	Strong		1
TS26: penile urethra	Strong		1
TS26: corpus cavernosum of glans penis	Present		1
TS26: pelvic girdle skeleton	Present		1
TS26: rectum	Present		1
TS26: reproductive system	Present		1
TS26: tail mesenchyme	Present		1
TS26: bladder	Present	Regionally restricted	1	Expression is caudal, absent in the subepithelial mesenchyme.
TS26: urethra	Present	Regionally restricted	1	Expression is strongest in the dense subepithelial mesenchyme at the junction of the Wolffian and Mullerian ducts with the urethra.
TS26: urinary system mesenchyme	Present	Regionally restricted	1	Expression is strongest in the dense subepithelial mesenchyme at the junction of the Wolffian and Mullerian ducts with the urethra.
TS26: embryo mesenchyme	Present	Regionally restricted	1	Expression is in the loose pelvic mesenchyme surrounding symphysis and rectum, extending into the ventral body wall, caudally into the perineum, and behind the rectum into the tail forming a loop around the rectum.
TS26: male reproductive system mesenchyme	Present	Regionally restricted	1	Expression is in the scrotal swelling mesenchyme and is strong covering the bud of the vesicular gland.
TS26: body wall mesenchyme	Present	Regionally restricted	1	Expression is in the ventral body wall.
TS26: abdominal segment of trunk	Present	Regionally restricted	1	Expression is in the perineum.
TS26: male reproductive system	Present	Regionally restricted	1	Expression is strong in the epithelium of the Wolffian ducts and is moderate in the fusion zone of the Wolffian and Mullerian ducts with the sinus ridges.
TS26: urothelium of bladder	Present	Regionally restricted	1	Expression is in the trigone close to the urethral outlet.
TS26: urothelium of bladder trigone	Present	Regionally restricted	1	Expression is in the trigone close to the urethral outlet.
TS26: rectum epithelium	Moderate	Regionally restricted	1	Expression is in the junctional zone between mucosa and ectodermal lining of the proctodeum.
TS26: detrusor muscle of bladder	Absent		1
TS26: paramesonephric duct	Absent		1
TS26: rectum mesenchyme	Absent		1
TS26: rectum muscularis mucosa	Absent		1
TS26: surface ectoderm of genital tubercle	Absent		1

Structure	Cell Type	Level	Pattern	Image	Note
TS25: embryo mesenchyme		Present	Regionally restricted	2	Expression is in a loop around the rectum.
TS25: genitourinary system		Present	Regionally restricted	2	Expression is in the loose mesenchyme of the Wolffian body and in the epithelium of the Wolffian ducts.
TS25: epididymis		Strong	Regionally restricted	2	Expression is in the tubules of the initial segment.
TS25: testis mesenchyme		Present		2
TS25: peritoneum		Present	Regionally restricted	2	Expression is in some epithelial cells corresponding to Douglas pouch epithelium and covering the umbilical artery and the Wolffian body.
TS25: germ cell of testis		Absent		2
TS25: Leydig cell	Leydig cell	Absent		2
TS25: Sertoli cell	Sertoli cell	Absent		2

Structure	Level	Pattern	Image	Note
TS25: embryo mesenchyme	Present	Regionally restricted	3	Expression is in a loop around the rectum.
TS25: epithelium of epididymis	Strong		3
TS25: epididymis	Strong	Regionally restricted	3	Expression is in the epithelium and is nearly absent in the wall layers.
TS25: male reproductive system	Present	Regionally restricted	3	Expression is in the mesenchyme surrounding the Wolffian and Mullerian ducts, is strong in the sinus epithelium, is weak in the Wolffian epithelium, and is absent in the Mullerian epithelium.
TS25: male reproductive system mesenchyme	Strong	Regionally restricted	3	Expression is in the mesenchyme surrounding the fusion zone of the Mullerian and Wolffian ducts.
TS25: ureter	Present	Regionally restricted	3	Expression is in a number of subepithelial mesenchymal cells inserted between the ureteral epithelium and the muscle layer.
TS25: ureteral smooth muscle layer	Absent		3
TS25: urothelium of ureter	Absent		3

Structure	Level	Pattern	Image	Note
TS25: male reproductive system mesenchyme	Strong	Regionally restricted	4	Expression is in the mesenchyme surrounding the fusion zone of the Mullerian and Wolffian ducts.
TS25: ureter	Present	Regionally restricted	4	Expression is in a number of subepithelial mesenchymal cells inserted between the ureteral epithelium and the muscle layer.
TS25: ureteral smooth muscle layer	Absent		4
TS25: urothelium of ureter	Absent		4

Structure	Level	Pattern	Image	Note
TS25: urethra of male	Strong	Regionally restricted	5	Expression is strong in the loose mesenchyme and is moderate in the muscle layer.
TS25: epithelium of dorsal prostate bud	Present		5
TS25: epithelium of lateral prostate bud	Present		5
TS25: epithelium of ventral prostate bud	Present		5
TS25: prostate bud	Strong	Regionally restricted	5	Expression is in the subepithelial mesenchyme.

Structure	Level	Pattern	Image	Note
TS26: epithelium of dorsal pelvic urethra of male	Strong	Regionally restricted	6	Expression is strong in the epithelium of the Wolffian ducts and is moderate in the fusion zone of the Wolffian and Mullerian ducts with the sinus ridges.
TS26: male reproductive system	Strong	Regionally restricted	6	Expression is strong in the epithelium of the Wolffian ducts and is moderate in the fusion zone of the Wolffian and Mullerian ducts with the sinus ridges.
TS26: paramesonephric duct	Absent		6

Structure	Level	Pattern	Image	Note
TS26: male reproductive system mesenchyme	Present	Regionally restricted	7	Expression is in the fusion zone of the Wolffian and Mullerian ducts with the sinus ridges.

Structure	Level	Pattern	Image	Note
TS26: epithelium of seminal vesicle	Present	Graded	8	Expression is at the base and became gradually negative towards the apex and its dorsal wall.

Structure	Level	Pattern	Image	Note
TS26: urethra epithelium	Present	Uniform	9
TS26: corpus cavernosum of glans penis	Present		9
TS26: epithelium of penile urethra	Present		9
TS26: urothelium of bladder trigone	Present		9
TS26: bulbourethral gland of male	Moderate		9
TS26: urethra of male	Strong	Regionally restricted	9	Expression is in the subepithelial mesenchyme and is stronger in the condensed plug of mesenchyme between the sinus ridges where the genital ducts fused with the sinus ridges.
TS26: mesenchyme of penile body	Strong		9

Structure	Level	Pattern	Image	Note
TS25: genitourinary system	Present	Regionally restricted	10	Expression is in the loose mesenchyme of the upper Wolffian bodies.
TS25: peritoneum	Present		10
TS25: ovary	Absent		10
TS25: paramesonephric duct	Absent		10

Structure	Level	Pattern	Image	Note
TS25: reproductive system mesenchyme	Present	Regionally restricted	11	Expression is surrounduing the fusion zone where the vaginal anlage develops between the urethra and rectum, genital ducts and urethra.
TS25: female reproductive system	Present	Regionally restricted	11	Expression is weak in the Wolffian ducts and is absent in the Muellerian ducts.
TS25: epithelium of urogenital sinus	Present	Regionally restricted	11	Expression is in the epithelium of the sinus ridges.
TS25: urethra of female	Present	Regionally restricted	11	Expression is in the epithelium of the sinus ridges.
TS25: muscle layer of pelvic urethra of female	Present		11

Structure	Level	Pattern	Image	Note
TS25: muscle layer of pelvic urethra of female	Present		12
TS25: urethra of female	Present		12

Structure	Level	Pattern	Image	Note
TS25: urethra of female	Strong	Regionally restricted	13	Expression is strong in the loose mesenchyme and is moderate in the muscle layer.
TS25: epithelium of urogenital sinus	Present	Regionally restricted	13	Expression is in the epithelium of the sinus ridges.
TS25: muscle layer of pelvic urethra of female	Present		13

Structure	Level	Pattern	Image	Note
TS25: mammary mesenchyme	Present		14

Structure	Level	Pattern	Image	Note
TS26: mammary mesenchyme	Present	Regionally restricted	15	Expression formed a contsriction around the neck of the epithelial bud.

Structure	Level	Pattern	Image	Note
TS26: cortical early proximal tubule	Present		16
TS26: parietal epithelium of mature renal corpuscle	Present		16
TS26: renal capsule	Present		16
TS26: mesenchyme of metanephros	Absent		16

Structure	Level	Pattern	Image	Note
TS25: trunk mesenchyme	Present	Regionally restricted	17	Expression is in the retroperitoneal mesenchyme surrounding the ureter.

Structure	Level	Pattern	Image	Note
TS25: ureter	Present	Regionally restricted	18	Expression is in a number of subepithelial mesenchymal cells inserted between the ureteral epithelium and the muscle layer.
TS25: ureteral smooth muscle layer	Absent		18
TS25: urothelium of ureter	Absent		18

Structure	Level	Pattern	Image	Note
TS25: urothelium of bladder trigone	Present		19
TS25: urothelium of ureter	Absent		19

Structure	Level	Pattern	Image	Note
TS25: gut dorsal mesentery	Present		20
TS25: trunk mesenchyme	Present	Regionally restricted	20	Expression is in loose adventitial mesenchyme surrounding organs such as rectum, bladder, autonomic ganglia, umbilical and vesical arteries, skeletal elements of the pelvic girdle and surrounding the ureters and ascending into the retroperitoneal space into the levels of the kidneys.

Structure	Level	Pattern	Image	Note
TS26: gut dorsal mesentery	Present		21
TS26: trunk mesenchyme	Present	Regionally restricted	21	Expression is in loose adventitial mesenchyme surrounding organs such as rectum, bladder, autonomic ganglia, umbilical and vesical arteries, skeletal elements of the pelvic girdle and surrounding the ureters and ascending into the retroperitoneal space into the levels of the kidneys.

Structure	Level	Pattern	Image	Note
TS26: gubernaculum of male	Present		22
TS26: peritoneum	Present	Regionally restricted	22	Expression is in some epithelial cells corresponding to Douglas pouch epithelium and covering the umbilical artery and the Wolffian body.
TS26: trunk mesenchyme	Present	Regionally restricted	22	Expression is in loose adventitial mesenchyme surrounding organs such as rectum, bladder, autonomic ganglia, umbilical and vesical arteries, skeletal elements of the pelvic girdle and surrounding the ureters and ascending into the retroperitoneal space into the levels of the kidneys. Expression is around the medial and lower mesenteric arteries extending into the root of the dorsal enteric mesenteries.
TS26: artery tunica adventitia	Absent		22