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Gene Expression Data
Assay Details
Assay
Reference: J:269344 Teng CS, et al., Altered bone growth dynamics prefigure craniosynostosis in a zebrafish model of Saethre-Chotzen syndrome. Elife. 2018 Oct 25;7:e37024
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6315209
Gene symbol: Sp7
Gene name: Sp7 transcription factor 7
Antibody: OSX Antibody (A-13): sc-22536-R
Detection system: Alexa Fluor 488
Results
Specimen 6C left WT: embryonic day 14.5 (more )
Note: Double labeled: green - Sp7; red - BrdU.
Structure Level Pattern Image Note
TS22: frontal bone primordium Present 6C left WT
TS22: parietal bone primordium Present 6C left WT
Specimen 6C left Mut: embryonic day 14.5; Tcf12tm3.1Zhu/Tcf12+, Twist1tm1Bhr/Twist1+ (more )
Note: Double labeled: green - Sp7; red - BrdU.
Structure Level Pattern Image Note
TS22: frontal bone primordium Present 6C left Mut Marked increase in the number of positive osteoblasts at the bones fronts predestined for the suture. Data was quantified in 6D, E, F.
TS22: parietal bone primordium Present 6C left Mut Marked increase in the number of positive osteoblasts at the bones fronts predestined for the suture. Data was quantified in 6D, E, F.
Specimen 6C right WT: embryonic day 16.5 (more )
Note: Double labeled: green - Sp7; red - BrdU.
Structure Level Pattern Image Note
TS24: parietal bone Present 6C right WT
Specimen 6C right Mut: embryonic day 16.5; Tcf12tm3.1Zhu/Tcf12+, Twist1tm1Bhr/Twist1+ (more )
Note: Double labeled: green - Sp7; red - BrdU.
Structure Level Pattern Image Note
TS24: parietal bone Present 6C right Mut Marked increase in the number of positive osteoblasts at the bones fronts predestined for the suture. Data was quantified in 6D, E, F.
Specimen 6C-S1A left upper: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS22: frontal bone primordium Present 6 S1A WT upper
TS22: parietal bone primordium Present 6 S1A WT upper
Specimen 6C-S1A right upper: embryonic day 14.5; Tcf12tm3.1Zhu/Tcf12+, Twist1tm1Bhr/Twist1+ (more )
Structure Level Pattern Image Note
TS22: frontal bone primordium Present 6 S1A Mut upper Thicker bone fronts, as marked by positive cells in the mutant. Data was quantified in S1B.
TS22: parietal bone primordium Present 6 S1A Mut upper Thicker bone fronts, as marked by positive cells in the mutant. Data was quantified in S1B.
Specimen 6C-S1A left lower: embryonic day 16.5 (more )
Structure Level Pattern Image Note
TS24: parietal bone Present 6 S1A WT lower
Specimen 6C-S1A right lower: embryonic day 16.5; Tcf12tm3.1Zhu/Tcf12+, Twist1tm1Bhr/Twist1+ (more )
Structure Level Pattern Image Note
TS24: parietal bone Present 6 S1A Mut lower Thicker bone fronts, as marked by positive cells in the mutant. Data was quantified in S1B.
Specimen 7C upper left: embryonic day 14.5 (more )
Note: Double labeled: green - Sp7; red - Gli1 (assay MGI:6315226).
Structure Level Pattern Image Note
TS22: frontal bone primordium Present 7C left upper
TS22: parietal bone primordium Present 7C left upper
Specimen 7C lower left: embryonic day 14.5; Tcf12tm3.1Zhu/Tcf12+, Twist1tm1Bhr/Twist1+ (more )
Note: Double labeled: green - Sp7; red - Gli1 (assay MGI:6315226).
Structure Level Pattern Image Note
TS22: frontal bone primordium Present 7C left lower
TS22: parietal bone primordium Present 7C left lower
Specimen 7C upper right: embryonic day 14.5 (more )
Note: Double labeled: green - Sp7; red - Grem1 (assay MGI:6315227).
Structure Level Pattern Image Note
TS22: frontal bone primordium Present 7C right upper
TS22: parietal bone primordium Present 7C right upper
Specimen 7C lower right: embryonic day 14.5; Tcf12tm3.1Zhu/Tcf12+, Twist1tm1Bhr/Twist1+ (more )
Note: Double labeled: green - Sp7; red - Grem1 (assay MGI:6315227).
Structure Level Pattern Image Note
TS22: frontal bone primordium Present 7C right lower
TS22: parietal bone primordium Present 7C right lower
Specimen 7D upper left: embryonic day 16.5 (more )
Note: Double labeled: green - Sp7; red - Gli1 (assay MGI:6315226).
Structure Level Pattern Image Note
TS24: parietal bone Present 7D left upper
Specimen 7D lower left: embryonic day 16.5; Tcf12tm3.1Zhu/Tcf12+, Twist1tm1Bhr/Twist1+ (more )
Note: Double labeled: green - Sp7; red - Gli1 (assay MGI:6315226).
Structure Level Pattern Image Note
TS24: parietal bone Present 7D left lower
Specimen 7D upper right: embryonic day 16.5 (more )
Note: Double labeled: green - Sp7; red - Grem1 (assay MGI:6315227).
Structure Level Pattern Image Note
TS24: parietal bone Present 7D right upper
Specimen 7D lower right: embryonic day 16.5; Tcf12tm3.1Zhu/Tcf12+, Twist1tm1Bhr/Twist1+ (more )
Note: Double labeled: green - Sp7; red - Grem1 (assay MGI:6315227).
Structure Level Pattern Image Note
TS24: parietal bone Present 7D right lower
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory