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Gene Expression Data
Assay Details
Assay
Reference: J:226501 Uchida Y, et al., Class 3 semaphorins negatively regulate dermal lymphatic network formation. Biol Open. 2015;4(9):1194-205
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6313605
Gene symbol: Prox1
Gene name: prospero homeobox 1
Results
Specimen 2C: embryonic day 17.5; Nrp2tm1.2Mom/Nrp2+ (more )
Note: Triple labeled: red - Prox1; green - Nrp2 (assay MGI:6313608); blue - Lyve1 (assay MGI:6313606).
Structure Level Pattern Image Note
TS25: limb skin Present 2C Expression was detected in lymphatic endothelial cells. Co-expression was noted with Nrp2 and Lyve1.
TS25: lymphatic vessel endothelium Present 2C Expression was detected in lymphatic endothelial cells. Co-expression was noted with Nrp2 and Lyve1.
Specimen 3A: embryonic day 15.5 (more )
Note: Triple labeled: red - Prox1; blue - Pecam1 (assay MGI:6313607); green - Lyve1 (assay MGI:6313606).
Structure Level Pattern Image Note
TS23: limb skin Present 3A Expression was detected in lymphatic endothelial cells.
TS23: lymphatic vessel Present 3A Expression was detected in lymphatic endothelial cells.
Specimen 3B: embryonic day 15.5 (more )
Note: Double labeled: red - Prox1; green - Lyve1 (assay MGI:6313606).
Structure Level Pattern Image Note
TS23: limb skin Present 3B , 3B' Expression was detected in lymphatic endothelial cells.
TS23: lymphatic vessel Present 3B , 3B' Expression was detected in lymphatic endothelial cells.
Specimen 3C: embryonic day 15.5; Nrp2tm1.2Mom/Nrp2tm1.2Mom (more )
Note: Double labeled: red - Prox1; green - Lyve1 (assay MGI:6313606).
Structure Level Pattern Image Note
TS23: limb skin Present 3C , 3C' Expression was detected in lymphatic endothelial cells.
TS23: lymphatic vessel Present 3C , 3C' Expression was detected in lymphatic endothelial cells.
Specimen 3D: embryonic day 15.5; Sema3ftm1.2Ddg/Sema3ftm1.2Ddg (more )
Note: Double labeled: red - Prox1; green - Lyve1 (assay MGI:6313606).
Structure Level Pattern Image Note
TS23: limb skin Present 3D' , 3D Expression was detected in lymphatic endothelial cells.
TS23: lymphatic vessel Present 3D' , 3D Expression was detected in lymphatic endothelial cells.
Specimen 3E: embryonic day 15.5; Sema3gtm1(KOMP)Vlcg/Sema3gtm1(KOMP)Vlcg (more )
Note: Double labeled: red - Prox1; green - Lyve1 (assay MGI:6313606).
Structure Level Pattern Image Note
TS23: limb skin Present 3E' , 3E Expression was detected in lymphatic endothelial cells.
TS23: lymphatic vessel Present 3E' , 3E Expression was detected in lymphatic endothelial cells.
Specimen 3F: embryonic day 15.5; Sema3ftm1.2Ddg/Sema3ftm1.2Ddg, Sema3gtm1(KOMP)Vlcg/Sema3gtm1(KOMP)Vlcg (more )
Note: Double labeled: red - Prox1; green - Lyve1 (assay MGI:6313606).
Structure Level Pattern Image Note
TS23: limb skin Present 3F' , 3F Expression was detected in lymphatic endothelial cells.
TS23: lymphatic vessel Present 3F' , 3F Expression was detected in lymphatic endothelial cells.
Specimen 4A: embryonic day 14.5 (more )
Note: Triple labeled: red - Prox1; blue - Lyve1 (assay MGI:6313606); green - pHH3.
Structure Level Pattern Image Note
TS22: limb skin Present 4A Expression was detected in lymphatic endothelial cells.
TS22: lymphatic system Present 4A Expression was detected in lymphatic endothelial cells.
Specimen 4B: embryonic day 14.5; Nrp2tm1.2Mom/Nrp2tm1.2Mom (more )
Note: Triple labeled: red - Prox1; blue - Lyve1 (assay MGI:6313606); green - pHH3.
Structure Level Pattern Image Note
TS22: limb skin Present 4B Expression was detected in lymphatic endothelial cells.
TS22: lymphatic system Present 4B Expression was detected in lymphatic endothelial cells.
Specimen 4C: embryonic day 14.5; Sema3ftm1.2Ddg/Sema3ftm1.2Ddg (more )
Note: Triple labeled: red - Prox1; blue - Lyve1 (assay MGI:6313606); green - pHH3.
Structure Level Pattern Image Note
TS22: limb skin Present 4C Expression was detected in lymphatic endothelial cells.
TS22: lymphatic system Present 4C Expression was detected in lymphatic endothelial cells.
Specimen 4D: embryonic day 14.5; Sema3gtm1(KOMP)Vlcg/Sema3gtm1(KOMP)Vlcg (more )
Note: Triple labeled: red - Prox1; blue - Lyve1 (assay MGI:6313606); green - pHH3.
Structure Level Pattern Image Note
TS22: limb skin Present 4D Expression was detected in lymphatic endothelial cells.
TS22: lymphatic system Present 4D Expression was detected in lymphatic endothelial cells.
Specimen 5C: embryonic day 15.5 (more )
Note: Double labeled: red - Prox1; green - Lyve1 (assay MGI:6313606).
Structure Level Pattern Image Note
TS23: limb skin Present 5C' , 5C Expression was detected in lymphatic endothelial cells.
TS23: lymphatic vessel Present 5C' , 5C Expression was detected in lymphatic endothelial cells.
Specimen 5D: embryonic day 15.5; Plxna1tm1Tmj/Plxna1tm1Tmj (more )
Note: Double labeled: red - Prox1; green - Lyve1 (assay MGI:6313606).
Structure Level Pattern Image Note
TS23: limb skin Present 5D , 5D' Expression was detected in lymphatic endothelial cells.
TS23: lymphatic vessel Present 5D , 5D' Expression was detected in lymphatic endothelial cells.
Specimen 5E: embryonic day 15.5; Plxna2tm1Hfu/Plxna2tm1Hfu (more )
Note: Double labeled: red - Prox1; green - Lyve1 (assay MGI:6313606).
Structure Level Pattern Image Note
TS23: limb skin Present 5E' , 5E Expression was detected in lymphatic endothelial cells.
TS23: lymphatic vessel Present 5E' , 5E Expression was detected in lymphatic endothelial cells.
Specimen 7A: embryonic day 11.5 (more )
Note: Double labeled: red - Prox1; green - Lyve1 (assay MGI:6313606).
Structure Level Pattern Image Note
TS19: cardiovascular system endothelium Present Regionally restricted 7A Prox1-positive lymphatic endothelial cell progenitors bud from the anterior cardinal vein and migrate towards the superficial skin.
Specimen 7B: embryonic day 11.5; Nrp2tm1.2Mom/Nrp2tm1.2Mom (more )
Note: Double labeled: red - Prox1; green - Lyve1 (assay MGI:6313606).
Structure Level Pattern Image Note
TS19: cardiovascular system endothelium Present Regionally restricted 7B Prox1-positive lymphatic endothelial cell progenitors bud from the anterior cardinal vein and migrate towards the superficial skin, as in wild type.
Specimen 7C: embryonic day 11.5; Sema3ftm1.2Ddg/Sema3ftm1.2Ddg, Sema3gtm1(KOMP)Vlcg/Sema3gtm1(KOMP)Vlcg (more )
Note: Double labeled: red - Prox1; green - Lyve1 (assay MGI:6313606).
Structure Level Pattern Image Note
TS19: cardiovascular system endothelium Present Regionally restricted 7C Prox1-positive lymphatic endothelial cell progenitors bud from the anterior cardinal vein and migrate towards the superficial skin, as in wild type.
Specimen 7D: embryonic day 15.5 (more )
Note: Double labeled: red - Prox1; green - Lyve1 (assay MGI:6313606).
Structure Level Pattern Image Note
TS23: back skin Present 7D Expression was detected in lymphatic endothelial cells.
TS23: lymphatic vessel Present 7D Expression was detected in lymphatic endothelial cells.
Specimen 7E: embryonic day 15.5; Nrp2tm1.2Mom/Nrp2tm1.2Mom (more )
Note: Double labeled: red - Prox1; green - Lyve1 (assay MGI:6313606).
Structure Level Pattern Image Note
TS23: back skin Present 7E Expression was detected in lymphatic endothelial cells.
TS23: lymphatic vessel Present 7E Expression was detected in lymphatic endothelial cells.
Specimen 7F: embryonic day 15.5; Sema3ftm1.2Ddg/Sema3ftm1.2Ddg, Sema3gtm1(KOMP)Vlcg/Sema3gtm1(KOMP)Vlcg (more )
Note: Double labeled: red - Prox1; green - Lyve1 (assay MGI:6313606).
Structure Level Pattern Image Note
TS23: back skin Present 7F Expression was detected in lymphatic endothelial cells.
TS23: lymphatic vessel Present 7F Expression was detected in lymphatic endothelial cells.
Specimen 7G: embryonic day 17.5 (more )
Note: Double labeled: red - Prox1; green - Lyve1 (assay MGI:6313606).
Structure Level Pattern Image Note
TS25: back skin Present 7G Expression was detected in lymphatic endothelial cells.
TS25: lymphatic vessel endothelium Present 7G Expression was detected in lymphatic endothelial cells.
Specimen 7H: embryonic day 17.5; Nrp2tm1.2Mom/Nrp2tm1.2Mom (more )
Note: Double labeled: red - Prox1; green - Lyve1 (assay MGI:6313606).
Structure Level Pattern Image Note
TS25: back skin Present 7H Expression was detected in lymphatic endothelial cells.
TS25: lymphatic vessel endothelium Present 7H Expression was detected in lymphatic endothelial cells.
Specimen 7I: embryonic day 17.5; Sema3ftm1.2Ddg/Sema3ftm1.2Ddg, Sema3gtm1(KOMP)Vlcg/Sema3gtm1(KOMP)Vlcg (more )
Note: Double labeled: red - Prox1; green - Lyve1 (assay MGI:6313606).
Structure Level Pattern Image Note
TS25: back skin Present 7I Expression was detected in lymphatic endothelial cells.
TS25: lymphatic vessel endothelium Present 7I Expression was detected in lymphatic endothelial cells.
Specimen 7J: embryonic day 17.5 (more )
Note: Double labeled: red - Prox1; green - Lyve1 (assay MGI:6313606).
Structure Level Pattern Image Note
TS25: limb skin Present 7J Expression was detected in lymphatic endothelial cells.
TS25: lymphatic vessel endothelium Present 7J Expression was detected in lymphatic endothelial cells.
Specimen 7K: embryonic day 17.5; Nrp2tm1.2Mom/Nrp2tm1.2Mom (more )
Note: Double labeled: red - Prox1; green - Lyve1 (assay MGI:6313606).
Structure Level Pattern Image Note
TS25: limb skin Present 7K Expression was detected in lymphatic endothelial cells.
TS25: lymphatic vessel endothelium Present 7K Expression was detected in lymphatic endothelial cells.
Specimen 7L: embryonic day 17.5; Sema3ftm1.2Ddg/Sema3ftm1.2Ddg, Sema3gtm1(KOMP)Vlcg/Sema3gtm1(KOMP)Vlcg (more )
Note: Double labeled: red - Prox1; green - Lyve1 (assay MGI:6313606).
Structure Level Pattern Image Note
TS25: limb skin Present 7L Expression was detected in lymphatic endothelial cells.
TS25: lymphatic vessel endothelium Present 7L Expression was detected in lymphatic endothelial cells.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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