Lyve1 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:226501 Uchida Y, et al., Class 3 semaphorins negatively regulate dermal lymphatic network formation. Biol Open. 2015;4(9):1194-205
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:6313606
Gene symbol:	Lyve1
Gene name:	lymphatic vessel endothelial hyaluronan receptor 1

Antibody:	Anti-Lyve1 (ALY7)
Assay notes:	Two antibodies were described MGI:5476514 and MGI:6313417. The one used in this assay was not specified, either could have been used.

Results

2C ©

Specimen 2C: embryonic day 17.5; Nrp2^tm1.2Mom/Nrp2⁺ (more

)
Note: Triple labeled: blue - Lyve1; red - Prox1 (assay MGI:6313605); green - Nrp2 (assay MGI:6313608).

Structure	Level	Pattern	Image	Note
TS25: limb skin	Present		2C	Expression was detected in lymphatic endothelial cells. Co-expression was noted with Nrp2 and Prox1.
TS25: lymphatic vessel endothelium	Present		2C	Expression was detected in lymphatic endothelial cells. Co-expression was noted with Nrp2 and Prox1.

2D ©

Specimen 2D: embryonic day 17.5; Nrp2^tm1.2Mom/Nrp2⁺ (more

)
Note: Triple labeled: blue - Lyve1; red - Pecam1 (assay MGI:6313607); green - Nrp2 (assay MGI:6313608).

Structure	Level	Pattern	Image	Note
TS25: limb skin	Present		2D	Expression was detected in lymphatic endothelial cells. Co-expression was noted with Nrp2.
TS25: lymphatic vessel endothelium	Present		2D	Expression was detected in lymphatic endothelial cells. Co-expression was noted with Nrp2.

3A ©

Specimen 3A: embryonic day 15.5 (more

)
Note: Triple labeled: green - Lyve1; red - Prox1 (assay MGI:6313605); blue - Pecam1 (assay MGI:6313607).

Structure	Level	Pattern	Image	Note
TS23: limb skin	Present		3A	Expression was detected in lymphatic endothelial cells.
TS23: lymphatic vessel	Present		3A	Expression was detected in lymphatic endothelial cells.

3B ©

Specimen 3B: embryonic day 15.5 (more

)
Note: Double labeled: green - Lyve1; red - Prox1 (assay MGI:6313605).

Structure	Level	Pattern	Image	Note
TS23: limb skin	Present		3B	Expression was detected in lymphatic endothelial cells.
TS23: lymphatic vessel	Present		3B	Expression was detected in lymphatic endothelial cells.

3C ©

Specimen 3C: embryonic day 15.5; Nrp2^tm1.2Mom/Nrp2^tm1.2Mom (more

)
Note: Double labeled: green - Lyve1; red - Prox1 (assay MGI:6313605).

Structure	Level	Pattern	Image	Note
TS23: limb skin	Present		3C	Expression was detected in lymphatic endothelial cells.
TS23: lymphatic vessel	Present		3C	Expression was detected in lymphatic endothelial cells.

3D ©

Specimen 3D: embryonic day 15.5; Sema3f^tm1.2Ddg/Sema3f^tm1.2Ddg (more

)
Note: Double labeled: green - Lyve1; red - Prox1 (assay MGI:6313605).

Structure	Level	Pattern	Image	Note
TS23: limb skin	Present		3D	Expression was detected in lymphatic endothelial cells.
TS23: lymphatic vessel	Present		3D	Expression was detected in lymphatic endothelial cells.

3E ©

Specimen 3E: embryonic day 15.5; Sema3g^{tm1(KOMP)Vlcg}/Sema3g^{tm1(KOMP)Vlcg} (more

)
Note: Double labeled: green - Lyve1; red - Prox1 (assay MGI:6313605).

Structure	Level	Pattern	Image	Note
TS23: limb skin	Present		3E	Expression was detected in lymphatic endothelial cells.
TS23: lymphatic vessel	Present		3E	Expression was detected in lymphatic endothelial cells.

3F ©

Specimen 3F: embryonic day 15.5; Sema3f^tm1.2Ddg/Sema3f^tm1.2Ddg, Sema3g^{tm1(KOMP)Vlcg}/Sema3g^{tm1(KOMP)Vlcg} (more

)
Note: Double labeled: green - Lyve1; red - Prox1 (assay MGI:6313605).

Structure	Level	Pattern	Image	Note
TS23: limb skin	Present		3F	Expression was detected in lymphatic endothelial cells.
TS23: lymphatic vessel	Present		3F	Expression was detected in lymphatic endothelial cells.

4A ©

Specimen 4A: embryonic day 14.5 (more

)
Note: Triple labeled: blue - Lyve1; red - Prox1 (assay MGI:6313605); green - pHH3.

Structure	Level	Pattern	Image	Note
TS22: limb skin	Present		4A	Expression was detected in lymphatic endothelial cells.
TS22: lymphatic system	Present		4A	Expression was detected in lymphatic endothelial cells.

4B ©

Specimen 4B: embryonic day 14.5; Nrp2^tm1.2Mom/Nrp2^tm1.2Mom (more

)
Note: Triple labeled: blue - Lyve1; red - Prox1 (assay MGI:6313605); green - pHH3.

Structure	Level	Pattern	Image	Note
TS22: limb skin	Present		4B	Expression was detected in lymphatic endothelial cells.
TS22: lymphatic system	Present		4B	Expression was detected in lymphatic endothelial cells.

4C ©

Specimen 4C: embryonic day 14.5; Sema3f^tm1.2Ddg/Sema3f^tm1.2Ddg (more

)
Note: Triple labeled: blue - Lyve1; red - Prox1 (assay MGI:6313605); green - pHH3.

Structure	Level	Pattern	Image	Note
TS22: limb skin	Present		4C	Expression was detected in lymphatic endothelial cells.
TS22: lymphatic system	Present		4C	Expression was detected in lymphatic endothelial cells.

4D ©

Specimen 4D: embryonic day 14.5; Sema3g^{tm1(KOMP)Vlcg}/Sema3g^{tm1(KOMP)Vlcg} (more

)
Note: Triple labeled: blue - Lyve1; red - Prox1 (assay MGI:6313605); green - pHH3.

Structure	Level	Pattern	Image	Note
TS22: limb skin	Present		4D	Expression was detected in lymphatic endothelial cells.
TS22: lymphatic system	Present		4D	Expression was detected in lymphatic endothelial cells.

5B ©

Specimen 5B: embryonic day 16.5 (more

)
Note: Double labeled: green - Lyve1; red - Plxna1 (assay MGI:6313620).

Structure	Level	Pattern	Image	Note
TS24: limb skin	Present		5B	Expression was detected in lymphatic endothelial cells.
TS24: lymphatic vessel endothelium	Present		5B	Expression was detected in lymphatic endothelial cells.

5C ©

Specimen 5C: embryonic day 15.5 (more

)
Note: Double labeled: green - Lyve1; red - Prox1 (assay MGI:6313605).

Structure	Level	Pattern	Image	Note
TS23: limb skin	Present		5C	Expression was detected in lymphatic endothelial cells.
TS23: lymphatic vessel	Present		5C	Expression was detected in lymphatic endothelial cells.

5D ©

Specimen 5D: embryonic day 15.5; Plxna1^tm1Tmj/Plxna1^tm1Tmj (more

)
Note: Double labeled: green - Lyve1; red - Prox1 (assay MGI:6313605).

Structure	Level	Pattern	Image	Note
TS23: limb skin	Present		5D	Expression was detected in lymphatic endothelial cells.
TS23: lymphatic vessel	Present		5D	Expression was detected in lymphatic endothelial cells.

5E ©

Specimen 5E: embryonic day 15.5; Plxna2^tm1Hfu/Plxna2^tm1Hfu (more

)
Note: Double labeled: green - Lyve1; red - Prox1 (assay MGI:6313605).

Structure	Level	Pattern	Image	Note
TS23: limb skin	Present		5E	Expression was detected in lymphatic endothelial cells.
TS23: lymphatic vessel	Present		5E	Expression was detected in lymphatic endothelial cells.

7A ©

Specimen 7A: embryonic day 11.5 (more

)
Note: Double labeled: green - Lyve1; red - Prox1 (assay MGI:6313605).

Structure	Level	Pattern	Image	Note
TS19: lymph sac	Present		7A

7B ©

Specimen 7B: embryonic day 11.5; Nrp2^tm1.2Mom/Nrp2^tm1.2Mom (more

)
Note: Double labeled: green - Lyve1; red - Prox1 (assay MGI:6313605).

Structure	Level	Pattern	Image	Note
TS19: lymph sac	Present		7B

7C ©

Specimen 7C: embryonic day 11.5; Sema3f^tm1.2Ddg/Sema3f^tm1.2Ddg, Sema3g^{tm1(KOMP)Vlcg}/Sema3g^{tm1(KOMP)Vlcg} (more

)
Note: Double labeled: green - Lyve1; red - Prox1 (assay MGI:6313605).

Structure	Level	Pattern	Image	Note
TS19: lymph sac	Present		7C

7D ©

Specimen 7D: embryonic day 15.5 (more

)
Note: Double labeled: green - Lyve1; red - Prox1 (assay MGI:6313605).

Structure	Level	Pattern	Image	Note
TS23: back skin	Present		7D
TS23: lymphatic vessel	Present		7D

7E ©

Specimen 7E: embryonic day 15.5; Nrp2^tm1.2Mom/Nrp2^tm1.2Mom (more

)
Note: Double labeled: green - Lyve1; red - Prox1 (assay MGI:6313605).

Structure	Level	Pattern	Image	Note
TS23: back skin	Present		7E
TS23: lymphatic vessel	Present		7E

7F ©

Specimen 7F: embryonic day 15.5; Sema3f^tm1.2Ddg/Sema3f^tm1.2Ddg, Sema3g^{tm1(KOMP)Vlcg}/Sema3g^{tm1(KOMP)Vlcg} (more

)
Note: Double labeled: green - Lyve1; red - Prox1 (assay MGI:6313605).

Structure	Level	Pattern	Image	Note
TS23: back skin	Present		7F
TS23: lymphatic vessel	Present		7F

7G ©

Specimen 7G: embryonic day 17.5 (more

)
Note: Double labeled: green - Lyve1; red - Prox1 (assay MGI:6313605).

Structure	Level	Pattern	Image	Note
TS25: back skin	Present		7G
TS25: lymphatic vessel	Present		7G

7H ©

Specimen 7H: embryonic day 17.5; Nrp2^tm1.2Mom/Nrp2^tm1.2Mom (more

)
Note: Double labeled: green - Lyve1; red - Prox1 (assay MGI:6313605).

Structure	Level	Pattern	Image	Note
TS25: back skin	Present		7H
TS25: lymphatic vessel	Present		7H

7I ©

Specimen 7I: embryonic day 17.5; Sema3f^tm1.2Ddg/Sema3f^tm1.2Ddg, Sema3g^{tm1(KOMP)Vlcg}/Sema3g^{tm1(KOMP)Vlcg} (more

)
Note: Double labeled: green - Lyve1; red - Prox1 (assay MGI:6313605).

Structure	Level	Pattern	Image	Note
TS25: back skin	Present		7I
TS25: lymphatic vessel	Present		7I

7J ©

Specimen 7J: embryonic day 17.5 (more

)
Note: Double labeled: green - Lyve1; red - Prox1 (assay MGI:6313605).

Structure	Level	Pattern	Image	Note
TS25: limb skin	Present		7J
TS25: lymphatic vessel	Present		7J

7K ©

Specimen 7K: embryonic day 17.5; Nrp2^tm1.2Mom/Nrp2^tm1.2Mom (more

)
Note: Double labeled: green - Lyve1; red - Prox1 (assay MGI:6313605).

Structure	Level	Pattern	Image	Note
TS25: limb skin	Present		7K
TS25: lymphatic vessel	Present		7K

7L ©

Specimen 7L: embryonic day 17.5; Sema3f^tm1.2Ddg/Sema3f^tm1.2Ddg, Sema3g^{tm1(KOMP)Vlcg}/Sema3g^{tm1(KOMP)Vlcg} (more

)
Note: Double labeled: green - Lyve1; red - Prox1 (assay MGI:6313605).

Structure	Level	Pattern	Image	Note
TS25: limb skin	Present		7L
TS25: lymphatic vessel	Present		7L

7M ©

Specimen 7M: postnatal adult (more

)

Structure	Level	Pattern	Image	Note
TS28: ear skin	Present		7M
TS28: lymphatic vessel	Present		7M

7N ©

Specimen 7N: postnatal adult; Sema3f^tm1.2Ddg/Sema3f^tm1.2Ddg, Sema3g^{tm1(KOMP)Vlcg}/Sema3g^{tm1(KOMP)Vlcg} (more

)

Structure	Level	Pattern	Image	Note
TS28: ear skin	Present		7N
TS28: lymphatic vessel	Present		7N

8A ©

Specimen 8A: postnatal adult (more

)
Note: Triple labeled: green - Lyve1; blue - Pecam1 (assay MGI:6313607); red - Itga9 (assay MGI:6313709).

Structure	Level	Pattern	Image	Note
TS28: ear skin	Present		8A
TS28: lymphatic vessel	Present		8A

8B ©

Specimen 8B: postnatal adult; Sema3f^tm1.2Ddg/Sema3f^tm1.2Ddg, Sema3g^{tm1(KOMP)Vlcg}/Sema3g^{tm1(KOMP)Vlcg} (more

)
Note: Triple labeled: green - Lyve1; blue - Pecam1 (assay MGI:6313607); red - Itga9 (assay MGI:6313709).

Structure	Level	Pattern	Image	Note
TS28: ear skin	Present		8B
TS28: lymphatic vessel	Present		8B

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