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Gene Expression Data
Assay Details
Assay
Reference: J:248902 De Tomasi L, et al., Mutations in GREB1L Cause Bilateral Kidney Agenesis in Humans and Mice. Am J Hum Genet. 2017 Nov 2;101(5):803-814
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6307085
Gene symbol: Six2
Gene name: sine oculis-related homeobox 2
Antibody: anti-Six2 (11562-1-AP)
Detection system: Secondary antibody coupled to Alexa Fluor 568
Results
Specimen 4D +/+: embryonic day 13.5 (more )
Note: Double labeled: red - Six2; green - Calb1 (assay MGI:6307082).
Structure Level Pattern Image Note
TS21: metanephros Present 4D upper right , 4D upper middle
Specimen 4D +/-: embryonic day 13.5; Greb1lem1Cjea/Greb1l+ (more )
Note: Double labeled: red - Six2; green - Calb1 (assay MGI:6307082).
Structure Level Pattern Image Note
TS21: metanephros Present 4D lower middle , 4D lower right A slight difference in branching compared to wild-type, although the volume of the kidneys was not significantly decreased.
Specimen S4A +/+: embryonic day 13.5 (more )
Note: Due to the high level of Six2 external background, the specific kidney staining was isolated using the manual surface tool and pseudo-colored in green.
Structure Level Pattern Image Note
TS21: metanephros Present S4A upper
Specimen S4A -/-: embryonic day 13.5; Greb1lem1Cjea/Greb1lem1Cjea (more )
Note: Due to the high level of Six2 external background, the specific kidney staining was isolated using the manual surface tool and pseudo-colored in green.
Structure Level Pattern Image Note
TS21: metanephros Absent S4A lower Absence of kidney.
Specimen S4B +/+: embryonic day 13.5 (more )
Note: Due to the high level of Six2 external background, the specific kidney staining was isolated using the manual surface tool and pseudo-colored in green.
Structure Level Pattern Image Note
TS21: metanephros Present S4B upper
Specimen S4B -/-: embryonic day 13.5; Greb1lem1Cjea/Greb1lem1Cjea (more )
Note: Due to the high level of Six2 external background, the specific kidney staining was isolated using the manual surface tool and pseudo-colored in green.
Structure Level Pattern Image Note
TS21: metanephros Absent S4B lower Absence of kidney.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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