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Gene Expression Data
Assay Details
Assay
Reference: J:241573 Pui HP, et al., Gonocytes-to-spermatogonia transition initiates prior to birth in murine testes and it requires FGF signaling. Mech Dev. 2017 Apr;144(Pt B):125-139
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6306311
Gene symbol: Foxo1
Gene name: forkhead box O1
Antibody: Anti-Foxo1
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 3A E17.5: embryonic day 17.5 (more )
Note: Triple labeled: green - Foxo1; red - Cdh1 (assay MGI:6305359); white - Laminin.
Structure Level Pattern Image Note
TS25: germ cell of testis Present 3A E17.5 FOXO1 , 3A E17.5 FOXO1+ECAD+Laminin Expression in gonocytes was mainly nuclear at E17.5.
Specimen 3A E18.5: embryonic day 18.5 (more )
Note: Triple labeled: green - Foxo1; red - Cdh1 (assay MGI:6305359); white - Laminin.
Structure Level Pattern Image Note
TS26: germ cell of testis Present 3A E18.5 FOXO1+ECAD+Laminin , 3A E18.5 FOXO1 Expression in gonocytes was underwent nuclear to cytoplasmic translocation at E18.5 The number of cells with nuclear only expression was low, while cytoplasmic only or cytoplasmic and nuclear increased.
Specimen 3A P0.5: postnatal day 0.5 (more )
Note: Triple labeled: green - Foxo1; red - Cdh1 (assay MGI:6305359); white - Laminin.
Structure Level Pattern Image Note
TS27: germ cell of testis Present 3A P0.5 FOXO1 , 3A P0.5 FOXO1+ECAD+Laminin Expression in gonocytes was either cytoplasmic only (majority) or cytoplasmic and nuclear.
Specimen 3A P1.5: postnatal day 1.5 (more )
Note: Triple labeled: green - Foxo1; red - Cdh1 (assay MGI:6305359); white - Laminin.
Structure Level Pattern Image Note
TS27: germ cell of testis Present 3A P1.5 FOXO1+ECAD+Laminin , 3A P1.5 FOXO1
Specimen 3C E17.5: embryonic day 17.5 (more )
Note: Double labeled: green - Foxo1; red - Gfra1 (assay MGI:6305361).
Structure Level Pattern Image Note
TS25: germ cell of testis Present 3C E17.5 FOXO1 , 3C E17.5 GFRAI+FOXO1+DAPI Expression in gonocytes was mainly nuclear at E17.5.
Specimen 3C E18.5: embryonic day 18.5 (more )
Note: Double labeled: green - Foxo1; red - Gfra1 (assay MGI:6305361).
Structure Level Pattern Image Note
TS26: germ cell of testis Present 3C E18.5 GFRAI+FOXO1+DAPI , 3C E18.5 FOXO1 Expression in gonocytes was underwent nuclear to cytoplasmic translocation at E18.5 The translocation is correlated with expression of Gfra1. The majority of gonocytes with cytoplasmic expression only also expressed Gfra1.
Specimen 3C P0.5: postnatal day 0.5 (more )
Note: Double labeled: green - Foxo1; red - Gfra1 (assay MGI:6305361).
Structure Level Pattern Image Note
TS27: germ cell of testis Present 3C P0.5 FOXO1 , 3C P0.5 GFRAI+FOXO1+DAPI Expression in gonocytes was either cytoplasmic only (majority) or cytoplasmic and nuclear.
Specimen 3C P1.5: postnatal day 1.5 (more )
Note: Double labeled: green - Foxo1; red - Gfra1 (assay MGI:6305361).
Structure Level Pattern Image Note
TS27: germ cell of testis Present 3C P1.5 FOXO1 , 3C P1.5 GFRAI+FOXO1+DAPI
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory