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Gene Expression Data
Assay Details
Assay
Reference: J:149237 Perez-Balaguer A, et al., Shh dependent and independent maintenance of basal midbrain. Mech Dev. 2009 May-Jun;126(5-6):301-13
Assay type: RNA in situ
MGI Accession ID: MGI:6304479
Gene symbol: Pou4f1
Gene name: POU domain, class 4, transcription factor 1
Probe: Pou4f1 probe2
Probe preparation: Antisense RNA
Results
Specimen 7C: embryonic day 12.5 (more )
Note: Double labeled: blue - Pou4f1; red - Pax6 (assay MGI:6304435).
Structure Level Pattern Image Note
TS20: midbrain mantle layer Present 7C There was a sharp boundary between the alar midbrain Pou4f1-positive staining and the alar diencephalon Pax6-positive staining. In the basal plate, the red nucleus was clearly labeled.
Specimen 7F: embryonic day 12.5; Shhtm2Amc/Shhtm2Amc, En1tm2(cre)Wrst/En1+ (more )
Note: Conditional mutant. Double labeled: blue - Pou4f1; red - Pax6 (assay MGI:6304435).
Structure Level Pattern Image Note
TS20: midbrain lateral wall Present Regionally restricted 7F In the conditional mutant, the magnocellular portion of the red nucleus was still present; the caudal portion showed a weaker expression.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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