Antibody:

anti-Nkx6-1 (F55A10)

Structure	Level	Pattern	Image	Note
TS17: midbrain ventricular layer	Present	Regionally restricted	6C	Two immunopositive domains were noted in the ventricular region.
TS17: midbrain mantle layer	Present	Regionally restricted	6C	Some early born motorneurons of the oculomotor complex were found in the mantle layer.

Structure	Level	Pattern	Image	Note
TS17: midbrain ventricular layer	Present	Regionally restricted	6F	The two immunopositive domains in the ventricular region were detected in the conditional mutant, but they were less obvious than in wild type.
TS17: midbrain mantle layer	Present	Regionally restricted	6F	Some immunopositive cells were detected.

Structure	Level	Pattern	Image	Note
TS20: midbrain ventricular layer	Present	Regionally restricted	6I	Two immunopositive domains were noted in the ventricular region.

Structure	Level	Pattern	Image	Note
TS20: midbrain ventricular layer	Present	Regionally restricted	6L	At E12.5, the dorsal ventricular expression domain was absent, but the ventral domain was maintained.
TS20: midbrain mantle layer	Absent		6L

Structure	Level	Pattern	Image	Note
TS20: midbrain	Present	Regionally restricted	7G	Expression was detected in a ventral expression domain.

Structure	Level	Pattern	Image	Note
TS20: midbrain	Present	Regionally restricted	7J	Expression was detected in the ventral domain.