Probe:	Igfbp5 cDNA6
Probe preparation:	Antisense RNA

Structure	Level	Pattern	Image	Note
TS15: 1st branchial arch	Strong		4A
TS15: fronto-nasal process	Strong		4A
TS15: somite	Strong	Regionally restricted	4A	Expression was detected in the caudal aspect of each somite.
TS15: embryo	Present	Regionally restricted	4A	Expression was detected in anterior embryo.
TS15: midbrain-hindbrain junction	Present		4A
TS15: migrating neural crest	Present		4A
TS15: limb bud	Absent		4A

Structure	Level	Pattern	Image	Note
TS17: apical ectodermal ridge	Present		4B
TS17: forelimb bud	Present	Regionally restricted	4B	Expression was detected in the ventral aspect.
TS17: hindlimb bud	Present	Regionally restricted	4B	Expression was detected in the ventral aspect.
TS17: central nervous system roof plate	Weak		4B

Structure	Level	Pattern	Image	Note
TS19: head	Present	Regionally restricted	4C	Expression in the head region was confined to around the eye.

Structure	Level	Pattern	Image	Note
TS19: forelimb bud	Strong	Regionally restricted	4D	Strong expression was detected around the entire limb dorso-ventral margin.

Structure	Level	Pattern	Image	Note
TS19: forelimb bud	Present	Regionally restricted	4E	Expression was detected in the dorsal ventral margin.

Structure	Level	Pattern	Image	Note
TS20: embryo ectoderm	Present	Regionally restricted	4F	The ectoderm of the body showed upregulated expression. The ectoderm covering the fore, mid and hindbrain did not show expression.

Structure	Level	Pattern	Image	Note
TS20: hindlimb	Present	Regionally restricted	4G	Expression was detected around the dorso-ventral margin.

Structure	Level	Pattern	Image	Note
TS21: limb	Present	Regionally restricted	4H	Expression was maintained at the dorso-ventral margin.
TS21: limb interdigital region	Present		4H

Structure	Level	Pattern	Image	Note
TS21: hindlimb interdigital region	Present		4I
TS21: hindlimb digit	Present	Regionally restricted	4I	Expression was detected in the proximal digit region.

Structure	Level	Pattern	Image	Note
TS22: limb interdigital region	Strong		4J
TS22: limb digit	Strong	Regionally restricted	4J	Expression was detected in the proximal digit region.

Structure	Level	Pattern	Image	Note
TS17: apical ectodermal ridge	Present	Regionally restricted	5A	Expression was detected in the ventral portion.
TS17: limb ectoderm	Present	Regionally restricted	5A	Expression was detected in the ventral portion.

Structure	Level	Pattern	Image	Note
TS19: handplate apical ectodermal ridge	Present	Widespread	5B	Expression was almost throughout the apical ectodermal ridge.
TS19: forelimb bud ectoderm	Weak	Regionally restricted	5B	Expression in ventral ectoderm was downregulated. Some expression was detected in dorsal ectoderm.
TS19: forelimb bud mesenchyme	Weak	Regionally restricted	5B	Expression was faint in the mesenchymal core of the limb.

Structure	Level	Pattern	Image	Note
TS20: limb	Present	Regionally restricted	5C	Expression was detected in flattened apical ectodermal ridge.

Structure	Level	Pattern	Image	Note
TS21: hand	Present	Regionally restricted	5D	In the forelimb autopod, expression was elevated in the ectoderm over interdigital regions compared to the ectoderm over the digits. Knots of expression were detected in ventral tendon-forming regions.

Structure	Level	Pattern	Image	Note
TS22: forelimb interdigital region	Present	Regionally restricted	5E	Expression was detected in the interdigital ectoderm.
TS22: hand	Present	Regionally restricted	5E	Knots of expression were detected in ventral tendon-forming regions.

Structure	Level	Pattern	Image	Note
TS19: apical ectodermal ridge	Present	Regionally restricted	6A	Expression was found to be abnormal in the apical ectodermal ridge. Discontinuous expression was noted over variable regions of the ridge predominantly in the posterior region.

Structure	Level	Pattern	Image	Note
TS19: forelimb bud	Present	Regionally restricted	6C	The mutant forelimb bud had minor lesions in expression.

Structure	Level	Pattern	Image	Note
TS19: apical ectodermal ridge	Present	Regionally restricted	6D	Large regions of the apical ectodermal ridge were non-expressing.

Structure	Level	Pattern	Image	Note
TS20: forelimb	Present	Regionally restricted	6E	Expression was continuous at the dorso-ventral margin.
TS20: hindlimb	Present	Regionally restricted	6E	Expression was continuous at the dorso-ventral margin.
TS20: hand	Present	Regionally restricted	6E	Expression was found in the proximal aspect of the hand plate.

Structure	Level	Pattern	Image	Note
TS20: autopod	Present	Regionally restricted	6F	Anterior and posterior regions showed upregulation of expression.
TS20: hand	Present	Regionally restricted	6F	Expression was found in the proximal aspect of the hand plate.

Structure	Level	Pattern	Image	Note
TS21: limb	Present	Widespread	6G	Expression was upregulated almost throughout the limbs.

Structure	Level	Pattern	Image	Note
TS21: hand	Present	Regionally restricted	6H	Only the posterior distal autopod was devoid of expression.

Structure	Level	Pattern	Image	Note
TS21: autopod	Weak	Regionally restricted	6I	Expression was significantly reduced in the dorsal half of the apical ectodermal ridge.

Structure	Level	Pattern	Image	Note
TS22: forelimb	Present	Regionally restricted	6J	Only the distal tips no longer express Igfbp5.
TS22: hindlimb	Present	Regionally restricted	6J	Only the distal tips no longer express Igfbp5.