Mir7-2 Northern blot Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:265657 Kleaveland B, et al., A Network of Noncoding Regulatory RNAs Acts in the Mammalian Brain. Cell. 2018 Jul 12;174(2):350-362.e17
Assay type:	Northern blot
MGI Accession ID:	MGI:6296806
Gene symbol:	Mir7-2
Gene name:	microRNA 7-2

Probe:	miR-7 LNA probe
Probe preparation:	labelled with radioactivity
Assay notes:	The mature microRNA is encoded at multiple sites in the genome.

Results

Image: 6C

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified	Amount	Genetic Background	Mutant Allele(s)	Sex
WT	P adult	TS28: cerebral cortex	Present	1-10 µg; total RNA	Not Specified		Not Specified
WT	P adult	TS28: cerebral cortex	Present	1-10 µg; total RNA	Not Specified		Not Specified
Cyrano-/-	P adult	TS28: cerebral cortex	Present (a)	1-10 µg; total RNA	B6.129S6(Cg)-Oip5os1^tm1.2Dpbl	Oip5os1^tm1.2Dpbl/Oip5os1^tm1.2Dpbl	Not Specified
Cyrano-/-	P adult	TS28: cerebral cortex	Present (b)	1-10 µg; total RNA	B6.129S6(Cg)-Oip5os1^tm1.2Dpbl	Oip5os1^tm1.2Dpbl/Oip5os1^tm1.2Dpbl	Not Specified
Mir7 DKO	P adult	TS28: cerebral cortex	Ambiguous	1-10 µg; total RNA	C57BL/6J-Mir7-1^em1Dpbl Mir7b^em1Dpbl	Mir7-1^em1Dpbl/Mir7-1^em1Dpbl, Mir7b^em1Dpbl/Mir7b^em1Dpbl	Not Specified
Mir7 DKO	P adult	TS28: cerebral cortex	Ambiguous	1-10 µg; total RNA	C57BL/6J-Mir7-1^em1Dpbl Mir7b^em1Dpbl	Mir7-1^em1Dpbl/Mir7-1^em1Dpbl, Mir7b^em1Dpbl/Mir7b^em1Dpbl	Not Specified
Cyrano-/-;Mir7 DKO	P adult	TS28: cerebral cortex	Present (c)	1-10 µg; total RNA	involves: 129S6/SvEvTac * C57BL/6J	Mir7-1^em1Dpbl/Mir7-1^em1Dpbl, Mir7b^em1Dpbl/Mir7b^em1Dpbl, Oip5os1^tm1.2Dpbl/Oip5os1^tm1.2Dpbl	Not Specified
Cyrano-/-;Mir7 DKO	P adult	TS28: cerebral cortex	Present (c)	1-10 µg; total RNA	involves: 129S6/SvEvTac * C57BL/6J	Mir7-1^em1Dpbl/Mir7-1^em1Dpbl, Mir7b^em1Dpbl/Mir7b^em1Dpbl, Oip5os1^tm1.2Dpbl/Oip5os1^tm1.2Dpbl	Not Specified

Notes:
(a) Expression level was 8-fold higher in mutant compared to wild type.
(b) Expression level was 7.5-fold higher in mutant compared to wild type.
(c) Expression level did not substantially exceed that of wild type.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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