About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:163555 Huang CC, et al., Inactivation of Dicer1 in Steroidogenic factor 1-positive cells reveals tissue-specific requirement for Dicer1 in adrenal, testis, and ovary. BMC Dev Biol. 2010;10:66
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6296584
Gene symbol: Nr5a1
Gene name: nuclear receptor subfamily 5, group A, member 1
Antibody: anti-SF1
Assay notes: Controls (CT) were either Dicer1 flox/flox (homozygous) or Dicer1 flox/+ (heterozygous) littermate embryos.
Results
Specimen 1B 16.5 dpc CT: embryonic day 16.5; Dicer1tm1Bdh/Dicer1tm1Bdh (more )
Note: Triple labeled: red - SF1 (Nr5a1); green - Th (assay MGI:6296591); blue - DAPI.
Structure Level Pattern Image Note
TS24: adrenal cortex Present 1B 16.5 dpc CT
Specimen 1B 16.5 dpc KO: embryonic day 16.5; Dicer1tm1Bdh/Dicer1tm1Bdh, Tg(Nr5a1-cre)2Klp/0 (more )
Note: Conditional mutant. Triple labeled: red - SF1 (Nr5a1); green - Th (assay MGI:6296591); blue - DAPI.
Structure Level Pattern Image Note
TS24: adrenal cortex Present 1B 16.5 dpc KO Authors report that differentiation of cortex occurred properly in mutant adrenal gland compared to control.
Specimen 1B 18.5 dpc CT: embryonic day 18.5; Dicer1tm1Bdh/Dicer1tm1Bdh (more )
Note: Triple labeled: red - SF1 (Nr5a1); green - Th (assay MGI:6296591); blue - DAPI.
Structure Level Pattern Image Note
TS26: adrenal cortex Present 1B 18.5 dpc CT
Specimen 1B 18.5 dpc KO: embryonic day 18.5; Dicer1tm1Bdh/Dicer1tm1Bdh, Tg(Nr5a1-cre)2Klp/0 (more )
Note: Conditional mutant. Triple labeled: red - SF1 (Nr5a1); green - Th (assay MGI:6296591); blue - DAPI.
Structure Level Pattern Image Note
TS26: adrenal cortex Weak 1B 18.5 dpc KO Decrease in number of expressing cells as adrenal development progressed.
Specimen 1B P0 CT: postnatal day 0; Dicer1tm1Bdh/Dicer1tm1Bdh (more )
Note: Triple labeled: red - SF1 (Nr5a1); green - Th (assay MGI:6296591); blue - DAPI.
Structure Level Pattern Image Note
TS27: adrenal cortex Present 1B P0 CT
Specimen 1B P0 KO: postnatal day 0; Dicer1tm1Bdh/Dicer1tm1Bdh, Tg(Nr5a1-cre)2Klp/0 (more )
Note: Conditional mutant. Triple labeled: red - SF1 (Nr5a1); green - Th (assay MGI:6296591); blue - DAPI.
Structure Level Pattern Image Note
TS27: adrenal cortex Weak 1B P0 KO Decrease in number of expressing cells as adrenal development progressed.
Specimen 1B P5 CT: postnatal day 5; Dicer1tm1Bdh/Dicer1tm1Bdh (more )
Note: Triple labeled: red - SF1 (Nr5a1); green - Th (assay MGI:6296591); blue - DAPI.
Structure Level Pattern Image Note
TS28: adrenal cortex Present 1B P5 CT
Specimen 1B P5 KO: postnatal day 5; Dicer1tm1Bdh/Dicer1tm1Bdh, Tg(Nr5a1-cre)2Klp/0 (more )
Note: Conditional mutant. Triple labeled: red - SF1 (Nr5a1); green - Th (assay MGI:6296591); blue - DAPI.
Structure Level Pattern Image Note
TS28: adrenal cortex Trace 1B P5 KO Expression almost completely lost at P5.
Specimen 7B CT: postnatal day 5; Dicer1tm1Bdh/Dicer1tm1Bdh (more )
Note: Triple labeled: red - SF1 (Nr5a1); green - TRA98 (Gcna assay MGI:6296596); blue - DAPI.
Structure Level Pattern Image Note
TS28: ovary Present Regionally restricted 7B CT Expression in somatic cells.
Specimen 7B KO: postnatal day 5; Dicer1tm1Bdh/Dicer1tm1Bdh, Tg(Nr5a1-cre)2Klp/0 (more )
Note: Conditional mutant. Triple labeled: red - SF1 (Nr5a1); green - TRA98 (Gcna assay MGI:6296596); blue - DAPI.
Structure Level Pattern Image Note
TS28: ovary Present Regionally restricted 7B KO Authors state expression patterns were indistinguishable between mutant control embryos.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
03/25/2025
MGI 6.24 		The Jackson Laboratory