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Gene Expression Data
Assay Details
Assay
Reference: J:163555 Huang CC, et al., Inactivation of Dicer1 in Steroidogenic factor 1-positive cells reveals tissue-specific requirement for Dicer1 in adrenal, testis, and ovary. BMC Dev Biol. 2010;10:66
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6296584
Gene symbol: Nr5a1
Gene name: nuclear receptor subfamily 5, group A, member 1
Antibody: anti-SF1
Assay notes: Controls (CT) were either Dicer1 flox/flox (homozygous) or Dicer1 flox/+ (heterozygous) littermate embryos.
Results
Specimen 1B 16.5 dpc CT: embryonic day 16.5; Dicer1tm1Bdh/Dicer1tm1Bdh (more )
Note: Triple labeled: red - SF1 (Nr5a1); green - Th (assay MGI:6296591); blue - DAPI.
Structure Level Pattern Image Note
TS24: adrenal cortex Present 1B 16.5 dpc CT
Specimen 1B 16.5 dpc KO: embryonic day 16.5; Dicer1tm1Bdh/Dicer1tm1Bdh, Tg(Nr5a1-cre)2Klp/0 (more )
Note: Conditional mutant. Triple labeled: red - SF1 (Nr5a1); green - Th (assay MGI:6296591); blue - DAPI.
Structure Level Pattern Image Note
TS24: adrenal cortex Present 1B 16.5 dpc KO Authors report that differentiation of cortex occurred properly in mutant adrenal gland compared to control.
Specimen 1B 18.5 dpc CT: embryonic day 18.5; Dicer1tm1Bdh/Dicer1tm1Bdh (more )
Note: Triple labeled: red - SF1 (Nr5a1); green - Th (assay MGI:6296591); blue - DAPI.
Structure Level Pattern Image Note
TS26: adrenal cortex Present 1B 18.5 dpc CT
Specimen 1B 18.5 dpc KO: embryonic day 18.5; Dicer1tm1Bdh/Dicer1tm1Bdh, Tg(Nr5a1-cre)2Klp/0 (more )
Note: Conditional mutant. Triple labeled: red - SF1 (Nr5a1); green - Th (assay MGI:6296591); blue - DAPI.
Structure Level Pattern Image Note
TS26: adrenal cortex Weak 1B 18.5 dpc KO Decrease in number of expressing cells as adrenal development progressed.
Specimen 1B P0 CT: postnatal day 0; Dicer1tm1Bdh/Dicer1tm1Bdh (more )
Note: Triple labeled: red - SF1 (Nr5a1); green - Th (assay MGI:6296591); blue - DAPI.
Structure Level Pattern Image Note
TS27: adrenal cortex Present 1B P0 CT
Specimen 1B P0 KO: postnatal day 0; Dicer1tm1Bdh/Dicer1tm1Bdh, Tg(Nr5a1-cre)2Klp/0 (more )
Note: Conditional mutant. Triple labeled: red - SF1 (Nr5a1); green - Th (assay MGI:6296591); blue - DAPI.
Structure Level Pattern Image Note
TS27: adrenal cortex Weak 1B P0 KO Decrease in number of expressing cells as adrenal development progressed.
Specimen 1B P5 CT: postnatal day 5; Dicer1tm1Bdh/Dicer1tm1Bdh (more )
Note: Triple labeled: red - SF1 (Nr5a1); green - Th (assay MGI:6296591); blue - DAPI.
Structure Level Pattern Image Note
TS28: adrenal cortex Present 1B P5 CT
Specimen 1B P5 KO: postnatal day 5; Dicer1tm1Bdh/Dicer1tm1Bdh, Tg(Nr5a1-cre)2Klp/0 (more )
Note: Conditional mutant. Triple labeled: red - SF1 (Nr5a1); green - Th (assay MGI:6296591); blue - DAPI.
Structure Level Pattern Image Note
TS28: adrenal cortex Trace 1B P5 KO Expression almost completely lost at P5.
Specimen 7B CT: postnatal day 5; Dicer1tm1Bdh/Dicer1tm1Bdh (more )
Note: Triple labeled: red - SF1 (Nr5a1); green - TRA98 (Gcna assay MGI:6296596); blue - DAPI.
Structure Level Pattern Image Note
TS28: ovary Present Regionally restricted 7B CT Expression in somatic cells.
Specimen 7B KO: postnatal day 5; Dicer1tm1Bdh/Dicer1tm1Bdh, Tg(Nr5a1-cre)2Klp/0 (more )
Note: Conditional mutant. Triple labeled: red - SF1 (Nr5a1); green - TRA98 (Gcna assay MGI:6296596); blue - DAPI.
Structure Level Pattern Image Note
TS28: ovary Present Regionally restricted 7B KO Authors state expression patterns were indistinguishable between mutant control embryos.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/30/2024
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