Probe:	Raldh2 probe14
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS16: mammary line	Strong	Punctate	S1A , 1A	Strong expression in punctate pattern along flank between limb buds in mammary line forming region.
TS16: somite	Present	Regionally restricted	S1A , 1A	Expression in dorsal parts of cervical somites.
TS16: tail somite	Present		1A , S1A

Structure	Level	Pattern	Image	Note
TS17: mammary line	Strong	Punctate	1B , S1B	Strong expression in punctate pattern along flank between limb buds in mammary line forming region.
TS17: somite	Present	Regionally restricted	S1B , 1B	Expression in dorsal parts of cervical somites.
TS17: tail somite	Present		S1B , 1B
TS17: intraembryonic coelom	Present	Regionally restricted	1B , S1B	Expression in dorsal lining of intraembryonic coelom.

Structure	Level	Pattern	Image	Note
TS16: somite	Present	Punctate	1A'	Punctate pattern of expression in ventral hypaxial extensions of thoracic somites.

Structure	Level	Pattern	Image	Note
TS16: somite	Present	Punctate	1A''	Punctate pattern of expression in ventral hypaxial extensions of thoracic somites.
TS16: surface ectoderm	Present	Regionally restricted	1A''	Expression in localized regions of ectoderm including that associated with 3rd mammary gland.
TS16: intraembryonic coelom	Present	Regionally restricted	1A''	Expression in dorsal lining of intraembryonic coelom.

Structure	Level	Pattern	Image	Note
TS18: limb bud	Present		S1C
TS18: peritoneum	Present		S1C
TS18: mammary line	Absent		S1C	No expression in flank or in mammary gland.

Structure	Level	Pattern	Image	Note
TS19: limb bud	Present		S1D
TS19: peritoneum	Present		S1D
TS19: mammary placode	Absent		S1D	No expression in flank or in mammary gland.

Structure	Level	Pattern	Image	Note
TS20: limb interdigital region	Present		S1E

Structure	Level	Pattern	Image	Note
TS20: limb	Present		S1E'
TS20: peritoneum	Present		S1E'
TS20: mammary placode	Absent		S1E'	No expression in flank or in mammary gland.