Probe:	Ahnak cDNA2
Probe preparation:	Antisense labelled with P33 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS10: extraembryonic ectoderm	Present		3A
TS10: extraembryonic mesoderm	Present		3A
TS10: embryo	Absent		3A

Structure	Level	Pattern	Image	Note
TS11: amnion	Present		3B	highest levels
TS11: chorion	Present		3B	highest levels

Structure	Level	Pattern	Image	Note
TS11: amnion	Present		3C	highest levels
TS11: chorion	Present		3C	highest levels
TS11: extraembryonic mesoderm	Strong		3C

Structure	Level	Pattern	Image	Note
TS11: amnion	Present		3D	highest levels
TS11: chorion	Present		3D	highest levels

Structure	Level	Pattern	Image	Note
TS11: blood island	Present		3E	reduced expression
TS11: allantois mesoderm	Present	Regionally restricted	3E	Expressed in a subset of cells.
TS11: exocoelomic cavity	Present	Regionally restricted	3E	Expressed in the mesothelium lining the exocoelomic cavity.
TS11: amnion ectoderm	Present		3E
TS11: amnion mesoderm	Present		3E
TS11: chorion ectoderm	Present		3E
TS11: chorion mesoderm	Present		3E
TS11: parietal endoderm	Present	Regionally restricted	3E	Expressed in a subset of cells.

Structure	Level	Pattern	Image	Note
TS11: exocoelomic cavity	Present	Regionally restricted	3F	Expressed in the mesothelium lining the exocoelomic cavity.
TS11: blood island	Present		3F	reduced expression
TS11: parietal endoderm	Present	Regionally restricted	3F	Expressed in a subset of cells.
TS11: amnion ectoderm	Present		3F
TS11: amnion mesoderm	Present		3F
TS11: chorion ectoderm	Present		3F
TS11: chorion mesoderm	Present		3F

Structure	Level	Pattern	Image	Note
TS13: amnion mesenchyme	Present		4A
TS13: yolk sac mesenchyme	Present		4A
TS13: facial neural crest	Present		4A	cranial neural crest

Structure	Level	Pattern	Image	Note
TS13: heart mesentery	Present		4B
TS13: trunk mesenchyme	Present	Regionally restricted	4B	Expressed in the mesenchymal cells within the body wall surrounding the heart.

Structure	Level	Pattern	Image	Note
TS17: 1st branchial arch mandibular component	Present	Regionally restricted	4D	Highest expression in the mandible.
TS17: 1st branchial arch maxillary component	Present	Regionally restricted	4D	Highest expression in the upper palate.
TS17: pharynx	Present	Regionally restricted	4D	Highest expression in the tongue.

Structure	Level	Pattern	Image	Note
TS20: mandible primordium	Present		4E	Highest expression.
TS20: primary palate	Present		4E	Highest expression.
TS20: tongue	Present		4E	Highest expression.
TS20: tail	Present		4E

Structure	Level	Pattern	Image	Note
TS20: mandible primordium	Present		4F	Highest expression.
TS20: primary palate	Present		4F	Highest expression.
TS20: tongue	Present		4F	Highest expression.
TS20: head mesenchyme	Present	Regionally restricted	4F	Expressed in a subset of craniofacial mesenchyme, similar to that of Notch2, but somewhat more spatially restricted.
TS20: central nervous system	Weak	Regionally restricted	4F	Faintly expressed in a small subset of neural cells.

Structure	Level	Pattern	Image	Note
TS17: tail	Present

Structure	Level	Pattern	Image	Note
TS20: genital tubercle	Present
TS20: tail	Present
TS20: ventricle cardiac muscle	Present
TS20: skin	Present	Regionally restricted		ventral skin

Structure	Level	Pattern	Image	Note
TS28: decidua	Present