Probe:	HGF/SF cDNA2 (clone P48)
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS8: embryo	Absent			No expression before onset of gastrulation.

Structure	Level	Pattern	Image	Note
TS9: primitive streak	Strong		1A	At onset of gastrulation, strong expression in region of the developing primitive streak.

Structure	Level	Pattern	Image	Note
TS10: primitive streak	Present	Regionally restricted	1D	Expression follows the spatial progression of the streak and becomes more localized in intermediate and rostral part of the streak.
TS10: node	Present		1D	Starting at the late primitive streak, expression in region corresponding to the node.

Structure	Level	Pattern	Image	Note
TS10: embryo mesoderm	Present	Regionally restricted		Expression in the area of mesoderm from which the primordium of the heart will differentiate.
TS10: definitive endoderm	Present	Regionally restricted		Expression in foregut endoderm.

Structure	Level	Pattern	Image	Note
TS11: allantois	Absent

Structure	Level	Pattern	Image	Note
TS11: node	Present		1G
TS11: neural groove	Present	Regionally restricted	1G	Expression localized in intermediate and rostral part of neural groove, corresponding to area where notochord is forming.
TS11: notochordal plate	Present		1G
TS11: definitive endoderm	Present	Regionally restricted	1G	Expression in the prospective foregut.
TS11: future brain	Absent		1G	No expression in prospective forebrain.

Structure	Level	Pattern	Image	Note
TS12: allantois	Present			Expression becomes detectable in allantois.
TS12: common atrial chamber	Strong	Regionally restricted		High levels of expression in common atrium and ventricle. Expression overlaps with c-met in both myocardial and endocardial tissues.
TS12: primitive ventricle	Strong	Regionally restricted		High levels of expression in common atrium and ventricle. Expression overlaps with c-met in both myocardial and endocardial tissues.
TS12: somite	Present	Uniform		Expression evenly distributed over newly formed somites, overlapping with that of c-met.

Structure	Level	Pattern	Image	Note
TS16: 1st branchial arch	Present		1J
TS16: 2nd branchial arch	Present		1J
TS16: 3rd branchial arch	Present		1J
TS16: heart	Present
TS16: limb bud	Present

Structure	Level	Pattern	Image	Note
TS10: primitive streak	Present	Regionally restricted	2A	Expression along the primitive streak, confined to mesodermal cells of the streak
TS10: primitive endoderm	Present			Expression in the visceral endoderm.
TS10: embryo ectoderm	Absent			No expression in ectodermal layer.
TS10: embryo mesoderm	Present	Regionally restricted		Expression in the notochordal plate.